STUDIES ON THE ISOLATION PURIFICATION AND CHARACTERIZATION OF THE FUCOIDAN-GALACTOSAN SULFATE (FGS) FROM LAMINARIA JAPONICA

1INTRODUCTIONFGSanditsderivationpossessremarkablemedicinalvalues:antitumouseffect,antiviralactivity,immunomodulation,bloodfatregulation,andhypoglycemia.Studiesonthepolysaccharidefromlaminariajaponicastartedatthebeginningof20thcentury.Thoughalginicacidfromlaminariajaponicahasalreadybeenindustrialized[1],peopledonotknownclearlyaboutlaminarinandfucoidan[2]becauseofrestrictionintheisolationandpurificationmethods[3].Weusedenzymolysistechnique,modernchromatographymethods,andspectralanalysistos…     

Simultaneous determination of phytochemicals and low-weight organic acids in Sophora japonica bud and premium noni juice through column-switching liquid chromatography connected with ion chromatography system

In this work, a liquid chromatography connected with an ion chromatography by the column-switching technique was developed for the simultaneous determination of three phytochemicals (scopoletin, rutin, and quercetin), three anions (chloride, sulfate, and phosphoric), and seven organic acids (lactic, acetic, propionic, formic, malic, oxalic, and citric acid) in Chinese traditional herbs, S. japonica bud and premium noni juice. A concentration column was used to collect organic acids and anions, and then the concentrated analytes were eluted to an analytical column by column-switching technique in which target analytes could be separated successfully and detected simultaneously within 70?min. This method showed good linearity with the linear correlation coefficients in the range of 0.9971–1.0000. The relative standard deviations of the peak areas were all less than 5.63% (n?=?6). The limits of detection (LOD, S/N?=?3) of anions and organic acids were in the range of 0.0006–0.0857?mg/L, whereas phytochemicals were in the range of 0.0123–0.0197?mg/L. Recoveries were in the range of 86.53–117.01% for the spiked sample. This method was successfully applied to the determination of the low-weight organic acids and phytochemicals in Chinese traditional herbal medicines, S. japonica bud and premium noni juice.     

Rearranged Calamenene and Eudesmane Sesquiterpenoids from two Chinese Liverworts

Four novel rearranged calamenene sesquiterpenoids, 1 – 4 , and two eudesmane sesquiterpenoids, 5 and 6 , were isolated from the Chinese liverworts Chiloscyphus polyanthus (L.) and Bazzania japonica S. (Lac .) Lindb . Their structures and relative configurations were determined by chemical derivatization and in‐depth spectroscopic methods, especially 1D‐ and 2D‐NMR as well as HR‐MS analyses.     

Two New ent—Kaurane Diterpenoids from Isodon japonica

Two new ent-kauranoids,named maoyecrystals A (1) and B(2),were isolated from the EtOAc extract of the dried leaves of Isodon japonica (Burman f.) Hara collected in Tongbai mountains ,Henan Province,Their structures were determined on the basis of spectral data,especially by 2D NMR.     

大孔吸附树脂分离纯化金银花中黄酮类物质的研究

比较了AB-8、S-8、NKA-9和D-101 4种大孔吸附树脂对金银花提取液中黄酮类物质的吸附及解吸附性能.在静态吸附试验基础上,筛选出效果较好的D-101树脂进行动态试验研究,结果表明,D-101树脂在30℃下对金银花黄酮类物质的静态吸附-动态解吸较优的工艺参数为:上样液pH值2.46,解吸液为95%乙醇,解吸液的流速为3mL/min,pH值11,4.5BV解吸液即可完全洗脱被树脂吸附的黄酮类物质,其解吸率高达98.00%.在试验研究范围内,树脂吸附金银花黄酮是自发性放热过程,并且符合Langmuir方程,此外树脂对黄酮的吸附动力学可用Pseudo-second-order模型较好地拟合,其表观吸附速率常数为Kso℃=3.43×10-2g/(mg·min).     

Triterpenoid Glycosides of Fatsia japonica. II. Isolation and Structure of Glycosides from the Leaves

The previously known triterpenoid 3-O--L-arabinopyranosides of oleanolic and echinocystic acids and hederagenin, 3-O--D-glucopyranosyl-(12)-O--L-arabinopyranosides of oleanolic acid and hederagenin, in addition to 28-O--L-rhamnopyranosyl-(14)-O--D-glucopyranosyl-(16)-O--D-glucopyranosyl ethers of the 3-O--L-arabinopyranoside of hederagenin, and 3-O--D-glucopyranosyl-(12)-O--L-arabinopyranosides of oleanolic acid and hederagenin, respectively, are isolated from leaves ofFatsia japonica(Araliaceae). The structures of the glycosides are confirmed by chemical methods and ¹³ C NMR spectroscopy     

Simultaneous determination of phytoestrogens in different medicinal parts of Sophora japonica L. by capillary electrophoresis with electrochemical detection

A high-performance capillary electrophoresis with electrochemical detection (CE-ED) method has been developed for the determination of phytoestrogens from the pericarps and seeds of Sophora japonica L. in this work. Genistin, genistein, rutin, kaempferol and quercetin are important bioactive constituents in these plants. The effects of several factors such as the acidity and concentration of running buffer, the separation voltage, the applied potential and the injection time on the CE-ED procedure were investigated. Under the optimum conditions, the five analytes could be well separated within 18 min in a 75 cm length capillary (i.d. 25 microm) at the separation voltage of 16 kV in a 50 mmol L(-1) borax running buffer (pH 9.0). A 300 microm diameter carbon disk electrode was used as the working electrode positioned carefully opposite the outlet of the capillary in a wall-jet configuration at the potential of +950 mV (vs SCE). Detection limits (S/N = 3) ranged from 1.1 x 10(-7) to 2.8 x 10(-7) g mL(-1) for all fi ve analytes. This method was successfully used to analyse dried Flos sophorae immaturus, pericarps and seeds of dried Fructus sophorae after a relatively simple extraction procedure, and the assay results were satisfactory.     

Chemical constituents and antioxidant activity of essential oil and organic extract from the peel and kernel parts of Citrus japonica Thunb. (kumquat) from Iran

The constituents of essential oils and organic extracts from peel and kernels of Citrus japonica were analysed by GC and GC/MS. The content of essential oil in peel and kernel was 1.1 and 0.8% based on dry weight. The essential oil of C. japonica peel and kernel was characterised by a higher amount of limonene (51.0 and 47.1%) and germacrene D (12.1 and 6.3%), and the hexane extracts of its peel and kernel were characterised by a higher amount of dodecanol-1(12.9 and 20.8%) and linolenic acid (13.1 and 16.3%), respectively. The antioxidant activities of oils were evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) method. The results indicate that both oils from different parts of C. japonica possess considerable antioxidant activity. The fruit peel and kernel essential oil could thus be useful in the industries, chiefly in the food and pharmaceutical industries.     

Screening and mechanism study of components targeting DNA from the Chinese herb Lonicera japonica by liquid chromatography/mass spectrometry and fluorescence spectroscopy

A method which involves combination of centrifugal ultrafiltration sampling with high-performance liquid chromatography coupled with diode array detection and mass spectrometry (HPLC-DAD-MS) analysis was established for screening bioactive compounds binding to calf thymus deoxyribonucleic acid (ct-DNA) from the extracts of Lonicera japonica. Four compounds were screened out and identified as rutin, quercetin-3-O-glucoside, luteolin-7-O-glucoside and lonicerin, based on the comparison of retention time, UV spectra and MS data with those of standards. The DNA-binding capabilities of the latter three flavonoids were found for the first time. The binding mechanisms of rutin, quercetin-3-O-glucoside and luteolin-7-O-glucoside with ct-DNA at the molecular level were explored using acridine orange (AO) as a fluorescence probe. Groove binding is the most appropriate binding mode of these three flavonoids to DNA, according to ultraviolet absorption and fluorescence spectra, as well as melting temperature (T(m)) curves and viscosity measurements. The binding constants of rutin, quercetin-3-O-glucoside and luteolin-7-O-glucoside with DNA-AO complex were 3.81 x 10(3), 3.37 x 10(3) and 5.50 x 10(3) L/mol, respectively.     

Anthranilic acid derivatives from Inula japonica

Three new anthranilic acid derivatives, N-heneicosanoylanthranilic acid （1b）, N-tricosanoylanthranilic acid （1d）, N-tetracosanoylanthranilic acid （1e）, and two known N-arachidylanthranilic acid （1a） and N-docosanoylanthranilic acid （1c） were isolated from the aerial parts of lnula japonica Thunb. Their structures were established by spectroscopic and chemical methods.