催化动力学光度法测定痕量硫氰酸根的研究

基于硫氰酸根对碘氧化叠氮化钠的催化作用,建立了催化动力学光度法测定硫氰酸根的新方法.于352nm处分别测定催化体系和非催化体系的吸光度A和A_V,以△A=A_c-A定量,线性范围为1.0～700μg·L~-1,检出限1.0μg·L~-1.用于生物样品中痕量硫氰酸根的测定,结果满意.     

Zur Bestimmung der Beteiligung radioaktiv markierter Schmierstoffbestandteile an der Schichtbildung auf Reibkörpern)

Bei Untersuchungen zum Gleitverschleiß von Stahl mit einer Stift-Scheibe-Apparatur wurde als Modellschmierstoff Hexadecan mit Additiven, insbesondere Zn-Dicyclohexyldithiophosphat (ZnDTP), verwendet. Zur Bestimmung der Bildung festhaftender Schichten von Schmierstoffbestandteilen mit ⁶⁵ZnDTP, ZnDTP(¹⁴C) und Hexadecan (¹⁴C) in der Reibspur und außerhalb erwies sich bei Markierung mit ¹⁴C die Messung mit und ohne Abdcckung der Reibspur, mit dem γ, ß-Strahler ⁶³Zn die photometrische Auswertung der Autoradiographien als am besten geeignet. Die visuelle Beurteilung der Autoradiographien ergibt vor allem Aussagcn zur Gleichmäßigkeit der Schichten. ZnDTP (1% in Hexadecan) bildet z. B. ußerhalb der Reibspur etwa cine Monoschicht aus, in der Gleitspur dickere Schichten.     

Direct Photometry of Self-Reversed Lines Using a Double Exit Slit

In direct-current-arc spectrochemical analysis using a direct reading spectrometer, an alkali metal line which has a suitable sensitivity and is free from interference must often be rejected because of self-reversal in the required concentration range. The simplicity of alkali metal spectra greatly restricts the choice of analysis line, so that a means of utilizing self-reversed lines in direct photometry, where line-width methods such as those of Gerlach and Rollwagen¹ and Coheura² are not applicable, would be valuable. The analytical use of the ground state (UI) K 7664.9 Å line, which readily develops a strongly reversed profile, as shown in Fig. 1, is described in this note.     

褪色光度法测定羟自由基及常见食物的抗氧化活性

在用稀硫酸调节至pH 4的溶液中,生物染色剂玫瑰桃红R能被由Fenton反应产生的羟自由基(·OH)氧化而褪色.根据在520 nm波长处所测得的吸光度的消褪的程度(△A=A0-A1),实现了羟自由基存在量的光度测定,应用此方法还测定了自由基消除率,作为选择清除剂的判据;还测定了几种常见食品的抗氧化活性.从方法的操作和所测得的结果证明该方法具有操作简单、方便且稳定而经济.     

Quantifying Protein–Protein Interactions by Molecular Counting with Mass Photometry

Interactions between biomolecules control the processes of life in health and their malfunction in disease, making their characterization and quantification essential. Immobilization‐ and label‐free analytical techniques are desirable because of their simplicity and minimal invasiveness, but they struggle with quantifying tight interactions. Here, we show that mass photometry can accurately count, distinguish by molecular mass, and thereby reveal the relative abundances of different unlabelled biomolecules and their complexes in mixtures at the single‐molecule level. These measurements determine binding affinities over four orders of magnitude at equilibrium for both simple and complex stoichiometries within minutes, as well as the associated kinetics. These results introduce mass photometry as a rapid, simple and label‐free method for studying sub‐micromolar binding affinities, with potential for extension towards a universal approach for characterizing complex biomolecular interactions.     

4-(2-QUINOLYLAZO)-1, 3-DIHYDROXY-NAPHTHALENE——A NEW SENSITIVE ANALYTICAL REAGENT FOR TRACE COPPER DETERMINATION

The azo compound 4-(2-quinolylazo)-1,3-dihydroxynaphthalene (2-QADNm) has been synthesized and used as a new sensitive spectrophotometric and electroanalytical reagent for determination of trace amounts of copper. In dilute alkaline solutions, 2-QADNm shows a single-sweep polarographic wave at -0.95 V (vs. SCE). In the presence of copper(Ⅱ) two new waves appear at -0.52 and -0.79 V. Using the wave at-0.79 V, a trace amount of copper down to 10~(-8) mol/L can be determined. The electroanalytical characteristics of 2-QADNm and the electrochemical reaction mechanism of the copper(Ⅱ)-2-QADNm complex have been studied in detail by the use of various electrochemical techniques. In alkaline solutions, 2-QADNm forms red complex with copper(Ⅱ) which has an absorption peak at 526 nm, the molar absorptivity being 6.4×10~4 1·mol~(-1)·cm~(-1). In the presence of a non-ionic surfactant the molar absorptivity of this complex is 1.6×10~5 1·mol~(-1)·cm~(-1).     

三轴稳定GEO卫星漫反射光变特性

利用天文测光方法研究了地球同步轨道卫星可见光波段的反射光变特性.以三轴稳定地球同步轨道通讯卫星为研究样本,进行了可见光波段的光学非高分辨成像观测,并使用天文标准星进行定标.基于相位角序列的测光观测数据表明:在大相位角区域卫星光变曲线分布与漫反射光照数学模型计算结果有很好的一致性,以卫星主体和太阳能电池板的漫反射效应为主;而在小相位角区域与漫反射模型计算结果有明显的差异,为卫星的太阳帆板镜面反射效应影响所致.     

DDTC-光度法测定饮料中的微量铜

采用DDTC (铜试剂) -光度法测定饮料中的微量铜,研究了DDTC与铜显色反应的条件。结果表明,在pH 9. 5～1 1 . 0条件下,铜试剂与Cu(Ⅱ)生成棕黄色的络合物,其最大吸收波长442nm ,铜含量在0 .0 2 8～4 .70 0 μg/mL范围内服从比尔定律,表观摩尔吸光系数为1 . 3 2×1 0 3。     

氯酸钾-甲基红体系动力学光度法测定甲醛

在H2 SO4 介质中 ,利用甲醛催化氯酸钾氧化甲基红的褪色反应 ,建立了催化光度法测定痕量甲醛的新方法。方法的线性范围 0 0 4～ 0 80 μg·mL- 1,检出限 1 74× 1 0 - 9g·mL- 1。本法应用于化妆品中甲醛的测定 ,结果满意     

催化动力学光度法测定痕量苯酚

研究了在硫酸介质中苯酚对Ｉ＾－１催化Ｃｅ＾４＋－Ａｓ（Ⅲ）反应的抑制作用及其动力学条件。建立了用催化动力这光度法测定痕量苯酚的新方法，结果表明，在０．００１ｍｏｌ／ＬＣｅ（ＳＯ４）２，０．００１２５ｍｏｌ／Ｌ，Ａｓ２Ｏ３，０．０１ｍｇ／Ｌ，ＫＩ，０．０１２５ｍｏｌ／Ｌ，Ｈ２ＳＯ４和０．１３％（ｗ／ｖ）ＮａＣｌ溶液中测定苯酚，其表面摩尔吸光系数为２．１×１０＾５Ｌ．ｍｏｌ＾－１．ｃｍ＾－１，线性范围