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41.
The aim of this research work was the evaluation of stir-bar sorptive extraction (SBSE) in combination with an in situ derivatization to determine parabens (methylparaben, isopropylparaben, n-propylparaben, butylparaben and benzylparaben), triclosan and methyltriclosan in soil samples. This is the first time that this approach has been applied to the determination of these compounds in soil samples, providing important advantages over conventional extraction techniques, such as minimization of sampling handling, complete elimination of the use of organic solvents and simplification of the analytical procedure with reduced time consumption. The enriched target analytes were desorbed thermally using a thermodesorption system coupled to a gas chromatograph and a mass spectrometer. The optimized derivatization and SBSE extraction conditions, as well as the analytical characteristics of the method were obtained using spiked soil samples. The proposed methodology proved to be easy to use and sensitive, with limits of detection between 80 ng/kg and 1.06 μg/kg, and reproducibility values below 13%. The accuracy of the method was evaluated at two concentration levels, obtaining apparent recoveries between 91% and 110%. The matrix composition significantly influenced the extraction procedure, and a need to adopt a standard additions protocol is apparent. The analytes assayed were determined successfully in different environmental soil samples. 相似文献
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Tatyana V. Vakhrusheva Grigoriy D. Moroz Liliya Yu. Basyreva Ekaterina V. Shmeleva Sergey A. Gusev Elena V. Mikhalchik Ekaterina N. Grafskaia Ivan A. Latsis Oleg M. Panasenko Vassili N. Lazarev 《Molecules (Basel, Switzerland)》2022,27(18)
Cationic antimicrobial peptides (CAMPs) are considered as next-generation antibiotics with a lower probability of developing bacterial resistance. In view of potential clinical use, studies on CAMP biocompatibility are important. This work aimed to evaluate the behavior of synthetic short CAMPs (designed using bioinformatic analysis of the medicinal leech genome and microbiome) in direct contact with blood cells and plasma. Eight CAMPs were included in the study. Hemolysis and lactate dehydrogenase assays showed that the potency to disrupt erythrocyte, neutrophil and mononuclear cell membranes descended in the order pept_1 > pept_3 ~ pept_5 > pept_2 ~ pept_4. Pept_3 caused both cell lysis and aggregation. Blood plasma and albumin inhibited the CAMP-induced hemolysis. The chemiluminescence method allowed the detection of pept_3-mediated neutrophil activation. In plasma coagulation assays, pept_3 prolonged the activated partial thromboplastin time (APTT) and prothrombin time (at 50 μM by 75% and 320%, respectively). Pept_3 was also capable of causing fibrinogen aggregation. Pept_6 prolonged APTT (at 50 μM by 115%). Pept_2 was found to combine higher bactericidal activity with lower effects on cells and coagulation. Our data emphasize the necessity of investigating CAMP interaction with plasma. 相似文献
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Emerald ash borer (EAB), Agrilus planipennis Fairmaire, is an invasive insect that has killed millions of ash trees in Canada and the USA. (3Z)-Dodecen-12-olide is a known female-produced pheromone of this insect, and a concise, three-step synthesis of a 2:1 blend of (3Z)-dodecen-12-olide and (3E)-dodecen-12-olide starting from commercially available (2-carboxyethyl)triphenylphosphonium bromide and 10-bromo-1-decene is described. The key steps in this synthesis are a lithium-salt-free Wittig reaction and an intramolecular SN2 esterification. Both of these macrocyclic lactones are behaviorally active toward EAB, and the 2:1 blend whose synthesis is described here has the potential to be a detection agent, mating disruptor, or mass trapping agent, which could be used in the control of EAB. 相似文献
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A specific recognition material for bisphenol A (BPA) was prepared by using a covalent imprinting technique. A chloroform solution containing bisphenol A dimethacrylate as a template, ethylene glycol dimethacrylate as a cross-linking agent and 2,2′-azobis(isobutyronitrile) as an initiator was polymerized by UV initiation. When BPA was removed from the resulting polymer by hydrolysis of the ester bonds with aqueous sodium hydroxide, carboxylic acid residues were generated in the polymer. After the polymer was packed into a stainless steel column, retention factors of BPA and related compounds were measured. The imprinted polymer adsorbed BPA and structurally related compounds such as 4,4′-dihydroxybenzophenone, bis(4-hydroxyphenyl)sulfone and 4,4′-dihydroxybiphenyl. A typical association constant (Ka) was calculated to be 1.72×105 M−1 by Scatchard analysis. Interestingly, 17α- and 17β-estradiol were also bound to the imprinted polymer (Ka=1.68×105 M−1), indicating that the polymer could be used as artificial receptors for screening the compounds having estrogenic action. 相似文献
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Exposure to indoor air pollution (IAP) from the combustion of biomass fuels is an important cause of morbidity and mortality in developing countries. In the work discussed in this paper we evaluated the endocrine activity of soot particles from biomass fuels by using yeast bioassay. These pollutants could have -galactosidase activity with a relative potency (RP) about 10–7–10–9 that of estradiol. Soot particles from wood and straw combustion only partially induced -galactosidase activity whereas others produced fully inductive activity in the yeast assay system. These pollutants did not have estrogen antagonist and progesterone agonist activity within the defined concentration range. However, these pollutants require 2–4 orders of magnitude higher IC50 to inhibit the activity of progesterone in a similar dose-response manner to mifepristone. We therefore propose that the endocrine activity of some environmental pollutants may be because of inhibition of the progesterone receptor (hPR). GC–MS results showed that substituted polycyclic aromatic hydrocarbon (PAH) compounds, substituted phenolic compounds and derivatives, aromatic carbonyl compounds, and phytosteroids in these soot particles may be mimicking endogenous hormones. 相似文献
48.
Matthew J. Walworth R. Maggie Connatser Michael J. Sepaniak 《Journal of separation science》2009,32(17):2985-2992
Teratogenic, carcinogenic, and pervasive endocrine disrupting compounds (EDCs) in suspect water systems present an immediate threat to both the environment and potable water supplies. The US Environmental Protection Agency mandated research regarding suspect EDCs, personal care products, and pesticide pollution requires the use of suitable methods of analysis that can perform extraordinarily well in the field and show low “cost to benefit” ratios. Such methods must increasingly address the need for enhanced sensitivity and selectivity in interrogating complex mixtures. Here, several device and method optimization strategies, specific for these classes of compounds, are discussed and include, among others, the preconcentration of field samples utilizing high performance extraction disk cartridges. Matrix interferences and undetectable concentration levels are eliminated by the way of an RP extraction technique that is vital to obtaining detectable concentrations of target analytes in the ppb range. Established capillary EKC methods are modified and implemented on a short capillary for low nanoliter discrete injections, efficient separations, and detection with LIF of analyte mixtures. Efficient separations were achieved with plate counts ranging from 103 to 104. Analytes spiked into real sample matrices at ppb levels were easily separated and detected via LIF using a He/Cd laser operating at 325 nm. The presented techniques are valuable in determining both the presence and concentration of suspect contaminates in different water systems. The methods presented here could easily be extended to microfluidic platforms with little to no optimization for on‐site testing. 相似文献
49.
Identification of m=2 competent mode of complex magneto-hydro-dynamics activities during internal soft disruption based on singular value decomposition and tomography of soft-X-ray emission on the HT-7 tokamak 下载免费PDF全文
In this paper,the singular value decomposition(SVD) method as a filter is applied before the tomographic inversion of soft-X-ray emission.Series of ’filtered’ signals including specific chronos and topos are obtained.(Here,chronos and topos are the decomposed spatial vectors and the decomposed temporal vectors,respectively).Given specific magnetic flux function with coupling m = 1 and m = 2 modes,the line-integrated soft-X-ray signals at all chords have been obtained.Then m = 1 and m = 2 modes have been identified by tomography of simulated ’filtered’ signals extracted by the SVD method.Finaly,using the experimental line-integrated soft-X-ray signals,m = 2 competent mode of complex magnetohydrodynamics(MHD) activities during internal soft disruption is observed.This result demonstrates that m = 2 mode plays an important role in internal disruption(Here,m is the poloidal mode number). 相似文献
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