基于柔性碳纳米管压阻膜的无线加速度检测系统

通过对加速度检测的重大意义进行分析,得出了加速度检测仪的价值和意义,介绍了本系统的设计思路和应用前景。传感器采用了基于纳米技术的碳纳米管薄膜感应前端,拓宽了传感器设备的研发领域,同时应用无线传感技术,使系统进一步实用化。最后介绍了本系统的实验情况,以及具体应用的效果。     

光学频率梳非线性传输及其在相位噪声探测中的应用

光纤传递链路中相位噪声探测是基于光纤的精确射频标准传递系统中重要组成部分。为解决光纤链路相位噪声探测中节点反射干扰的问题, 本文基于广义非线性薛定谔方程, 理论上研究了窄带光学频率梳在色散位移光纤中的非线性光学传播特性。在理论分析的基础上, 结合密集波分复用架构, 提出了一种基于锁模光学频率梳进行光纤传递射频信号的相位噪声探测新技术, 克服了节点反射干扰, 给出了初步实验验证。     

Research on improving the accuracy of near infrared non-invasive hemoglobin detection

To optimize the accuracy of near-infrared non-invasive hemoglobin (Hb) clinical detection, high-performance instrument and prepossessing algorithm have been investigated. A near-infrared spectrophotometric system was constructed adopting InGaAs detector array with 16 pixels and plane grating spectrometer to obtain high signal noise ratio (SNR) spectral data. In our experiment, we applied the device independently to collect spectra data from 91 volunteers’ fingertips non-invasively. Two prediction tests were conducted to verify the effects of preprocessing algorithms improving the accuracy of near-infrared Hb detection and exclude the occasionality of satisfactory results in a single trial. Our non-invasive Hb detection methods were based on partial least squares (PLS). In each test, PLS, MSC coupled with PLS, DOSC coupled with PLS, three methods for non-invasive Hb detection, were analyzed respectively. The results of two trials showed that only DOSC & PLS performed excellently in both predictive ability and stability, obviously better than other two methods. Relative RMSEP was 6.16% in predicting test 1, 6.08% in predicting test 2, almost reaching the requirements of clinical application. It indicates that our independent-developed high-performance instrument and the method DOSC coupled with PLS are promising in non-invasive Hb detection clinical application.     

Comparison of Two Rhodamine-Polyamine Polystyrene Solid-phase Fluorescence Sensors for Hg(II) Detection Based on Theoretical Calculation

Two novel rhodamine-based polystyrene solid-phase fluorescence sensors PS-PA-I and PS-PA-II with different lengths of polyamines were synthesized for Hg(II) determination. The detection mechanism involving the Hg(II) chelation-induced spirocycle open of rhodamine was proposed with the aid of theoretical calculation. The stronger N-Hg bond and the longer polyamine chain in PS-PA-II led to a better selectivity, much higher and more quickly fluorescence response to Hg(II).     

Voltammetric Label‐free Immunosensors for the Diagnosis of Cystic Echinococcosis

Cystic echinococcosis (CE) or hydatid disease is a parasitic infection caused by Echinococcus granulosus. Early serodiagnosis and continuous monitoring of the disease is very important for medical treatment. Here, we report the detecting of both echinococcus antigen and antibody for the diagnosis of hydatid disease using square wave voltammetry (SWV)‐based immunosensors. The gold electrodes were functionalized using cysteamine/phenylene diisothiocyanate linkers and used for the immunosensors fabrication. The hydatid antigen and antibody immunosensors were constructed by the immobilization of either purified rabbit polyclonal antibody or recombinant antigen B (AgB), respectively on the functionalized gold electrodes surfaces. The detection in both cases was achieved by following the change in the SWV reduction peak current of the ferro/ferricyanide redox couple upon antibody or antigen binding. These immunosensors enabled the detection of echinococcus antigen and antibody within a concentration range of 1 pg.mL^?1 to 1 μg.mL^?1 with detection limits of 0.4 pg.mL^?1 and 0.3 pg.mL^?1, respectively. A preliminary application of the developed immunosensor was performed in spiked serum sample showing good recovery percentages ranging from 102 to 110 % for both hydatid antibody and antigen detection. This easy‐to‐use, sensitive, and low cost quantitative method holds great promise for the early diagnosis of hydatid disease and thus, better managements and treatment outcomes.     

Adjusting the selectivity of inorganic anion separation by capillary electrophoresis

The separation of the principal inorganic anions (bromide, carbonate, chlorate, chloride, fluoride, nitrate, nitrite, sulfate, phosphate) has been achieved using a capillary electrophoresis system with indirect UV detection at 260 nm. Several types of cationic surfactants (quaternary ammonium, phosphonium or methonium) were tested as electroosmotic flow modifiers and added to a chromatebased buffer prepared from potassium dichromate. The influence of many physicochemical parameters such as nature and concentration of cationic surfactant, buffer pH, dichromate concentration buffer, voltage and temperature upon the migration time of an analyte anion, peak efficiency, asymmetry factor, and finally resolution has been investigated. A linear relationship between the corrected area and the anion concentration in the 2.5–50 ppm range was obtained, thus allowing the quantitative analysis of anions in mineral water. Finally, by increasing the hydrodynamic injection time, the separation of inorganic anions at a low concentration level of 50 ng/ml was achieved without any loss of resolution.     

Capillary zone electrophoresis with UV-scanning detection for the analysis of metal species and their organic binding partners in biological systems

A capillary zone electrophoretic method with UV-scanning detection for the separation and identification of both free ligands and metal species is presented. The electrophoretic behavior of naturally occurring binding partners such as organic acids and amino acids was studied and compared with their metal-complexes. Copper(II) complexes of citrate and amino acids showed decreased electrophoretic mobilities and altered UV spectra. The optimized method was validated on ultrafiltered cow's milk and human milk samples. In cow's milk six low molecular weight substances, including citrate, orotate, and hippurate, could be separated. Metal supplementation with Cu(II), Ni(II), or Zn(II) decreased not only the citrate peak but also the orotate signal and in the case of copper(II) supplementation a new signal for the copper(II)-citrate complex appeared. In human milk samples various amino acids such as glutamate, phenylalanine, tyrosine, and tryptophan could be identified besides citrate. The electrophoretic mobilites and peak areas of the amino acids were also influenced by the metal supplementation.     

上转换发光免疫试纸条扫描检测系统研究

研制了一种以上转换发光材料作为标记物的扫描检测系统,有别于传统的测量方法,可做出定量和多重分析.扫描检测系统以扫描方式逐点测量经生物反应而结合上去的UCP颗粒的含量,记录整个免疫试纸条的信息,计算出被测样品中特定生物分子的浓度.实验结果表明,该系统对兔抗鼠疫免疫球蛋白（IgG）标准样品的检测灵敏度达到百ng/mL量级,且具有变异系数小于6%的重复性,符合生物免疫测定的要求.     

Canny算法的改进及其硬件的实现

传统的Canny边缘检测算法是先对图像进行平滑,然后利用二维滤波器计算图像的梯度值,从而得到梯度直方图。通过基于梯度直方图来选取双阈值,最后进行基于双阈值的非极大值抑制,得到边缘检测的图像。为了能很方便地在硬件中实现这种算法,对传统的Canny算法进行了适当的改进,利用模板来代替原算法中的卷积,从而使得该算法在FPGA(Field Programmable Gate Array)中可以完全利用原理图来实现。