新精神活性物质N-乙基氟胺酮盐酸盐的结构确定

近期我国部分地区相继出现尚未报道的一个新精神活性物质(New Psychoactive Substance, NPS)作为氯胺酮，氟胺酮替代品滥用情况，其化学结构及理化性质尚不明确，在毒品检验部门检验鉴定过程中易造成漏检，对公共健康和社会安全构成了严重威胁。为了明确此NPS的化学结构，本研究综合运用气相色谱-质谱联用(GC-MS)、高分辨质谱(HR-MS)、紫外光谱(UV)、红外光谱(IR)以及核磁共振(¹H NMR、¹³C NMR和2D-NMR)等技术并结合无机化学手段对在广西玉林缴获的该NPS进行了结构鉴定。最终，该NPS被鉴定为N-乙基氟胺酮盐酸盐，为一新化合物。相关研究结果可为该NPS的检验和鉴定提供数据参考，推动其列管，以减少对社会的危害。     

高效液相色谱手性固定相法拆分和测定乳酸左氧氟沙星中乳酸对映体

建立了高效液相色谱手性固定相法拆分和测定乳酸左氧氟沙星中乳酸对映体的方法。考察了CuSO₄溶液浓度和异丙醇比例对乳酸对映体和左氧氟沙星分离情况的影响。采用Phenomenex 3126(D)-Penicillamine手性色谱柱，优化流动相为2.5 mmol/L CuSO₄溶液-异丙醇(体积比为93∶7),乳酸对映体达到基线分离。以L-乳酸锂和水解后的D-乳酸为标准品，解决了直接采用乳酸为标准品导致测定结果有偏差的问题。L-乳酸和D-乳酸在20～400μg/mL范围内线性良好，相关系数R²分别为09999和0.9998。重复性实验得到的相对标准偏差(RSD)为0.7%～0.8%,回收率为98.77%～100.1%(RSD≤1.0%),准确度和精密度良好。该方法简便，适用于乳酸左氧氟沙星中乳酸对映体含量的测定。     

4D-proteomics-based breast cancer progressionEI北大核心CSCD

：The occurrence and development of breast cancer go through multiple processes，and the protein molecular information in its evolution is intricate and complex. The 4D-proteomics technique was used to investigate the tumor progression of MMTV-PyMT transgenic mice. The results of protein identification and differential expression analysis showed that a total of 5819 proteins were identified in 4D-proteomics analysis and 5667 proteins were quantifiable. Compared with 3-week-old tumors，a total of 270 differentially expressed proteins were found in the 5-week-old tumors，while 255 proteins in the 7-week-old tumors. We also detected 23 of proteins （such as，Srpk11 and Tinagl1）with gradual up-regulation and 35 of proteins （such as，Pdgfrb， Col1a2 and Col1a1）with gradual down-regulation during the tumor progression，including 3-，5-and 7-weeks. We also performed bioinformatics analysis，including subcellular localization，GO function analysis and pathway enrichment analysis. The results showed that the biological processes（immune regulation and lipid metabolism） and enrichment pathways （metabolic pathway）involved in differentially expressed proteins remained the same during the tumor progression，but some cellular components and molecular functions changed，such as，protein locations were extracellular region and membrane in 5-weeks-old tumors as well as cytoplasm in 7-weeks-old tumors. And important protein functions were protein binding functions in 5-weeks-old tumors as well as nucleotide binding in 7-weeks-old tumors. The acquired protein biomarkers and pathways could help with the accurate exploration of molecular mechanisms of tumor progression，screening of molecular targets of breast cancer，and evaluation of personalized treatment programs. © 2023, Youke Publishing Co.,Ltd. All rights reserved.     

铺砌(33,42)中直线上的D-点数

本文研究了阿基米德双铺砌(33,42)中的D-点问题.利用格点的性质,得到任一给定直线上的D-点数,推广了参考文献[2,3]中的结果.