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11.
Summary A rapid, robust and reproducible method providing excellent separation performance and simplicity using a 0.5% MC-4000 methyl
cellulosic sieving medium in DB-1 coated capillaries has been developed. The method is suitable for qualitative comparison
of DNA restriction profiles for fragments in the size range 100–1000 base pairs (bp). Efficiencies up to 8.5 million plates/m
(1057 bp fragment) were recorded. Peak resolution of 6 bp (291/297 bp, 335/341 bp) and 4 bp (238/242 bp, 341/345 bp) was achieved.
In addition, 1 bp partial resolution of 123/124 bp and 298/297 bp was obtained. Run-to-run (n=15), day-to-day (n=4), and capillary-to-capillary
(n=3) variations of 0.1–0.2% RSD, 0.3–0.5% RSD, and 0.1–0.3% RSD, respectively, were observed. The MC-4000 sieving matrix
was found to be better than hydroxypropyl methyl cellulose and hydroxypropyl cellulose, in terms of both performance and stability
in the DB-1 coated capillaries. The efficiency and resolution in DB-WAX capillaries were inferior to those obtained in DB-1
capillaries. The commercially available DB-1 capillaries were stable for months in the sieving medium at pH 8.3 and could
be regenerated to provide high efficiency after accidental current breaks. 相似文献
12.
LI Zi-jian LIU Ning LIU Zhi-qiang LIU Shu-ying HAN Qi-de ZHANG You-yi . Institute of Vascular Medicine Peking University Third Hospital Key Laboratory of Molecular Cardiovascular Sciences Ministry of Education Beijing P. R. China . Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changchun P. R. China 《高等学校化学研究》2006,22(3):319-323
Introduction Adrenoceptors(ARs),membersofthesuperfami lyofG proteincoupledreceptors,arethemostimpor tantreceptorsinvolvedincardiacregulation[1].ARsin cardiacmuscleincludebothαARsandβARssub types,whileinmostmammalianheart,βARsarethe predominantsubtypes.… 相似文献
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In this paper, by the use of a fixed point theorem, many new necessary and sufficient conditions for the existence of positive solutions in C[0,1]∩C1[0,1]∩C2(0,1) or C[0,1]∩C2(0,1) are presented for singular superlinear and sublinear second-order boundary value problems. Singularities at t=0, t=1 will be discussed. 相似文献
15.
Zhimin He 《Journal of Mathematical Analysis and Applications》2004,296(1):97-109
In this paper, by means of a new twin fixed-point theorem in a cone, the existence of at least two positive solutions of m-point boundary value problem for second order dynamic equations on time scales is considered. 相似文献
16.
Ansgar Jüngel 《Mathematische Nachrichten》1997,185(1):85-110
A multi-dimensional transient drift-diffusion model for (at most) three charged particles, consisting of the continuity equations for the concentrations of the species and the Poisson equation for the electric potential, is considered. The diffusion terms depend on the concentrations. Such a system arises in electrophoretic modeling of three species (neutrally, positively and negatively charged) and in semiconductor theory for two species (positively charged holes and negatively charged electrons). Diffusion terms of degenerate type are also possible in semiconductor modeling. For the initial boundary value problem with mixed Dirichlet - Neumann boundary conditions and general reaction rates, a global existence result is proved. Uniqueness of solutions follows in the Dirichlet boundary case if the diffusion terms are uniformly parabolic or if the initial and boundary densities are strictly positive. Finally, we prove that solutions exist which are positive uniformly in time and globally bounded if the reaction rates satisfy appropriate growth conditions. 相似文献
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马意海 《高校应用数学学报(A辑)》1998,13(2):197-202
本文对一类重要的混合单调算子证明了不动点的存在、唯一与逼近定理,并应用于研究动态规划中泛函方程的正解问题. 相似文献
20.
考虑一类p-Laplacian算子型泛函微分方程的奇异边值问题,利用锥不动点定理,得到了其正解及多个正解存在的充分条件. 相似文献