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In CZE, the adsorption of the proteins on the capillary wall is a common problem. This paper describes the simple method of utilizing zwitterionic buffer additives to improve the separation of proteins in untreated fused silica capillaries at neutral pH. Three kinds of zwitterion are evaluated in the separation of acidic, neutral, and basic proteins, including their effect on protein efficiency, mobility, separation, and resolution; the difference between the effects of the different additives are also highlighted. The method has proved to be a possible means of reducing protein adsorption, especially for basic proteins. 相似文献
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Based on the Kachanov method and the alternating iteration technique, a new method is proposed to deal with the problem of the strongly interacted multiple cracks in an infinite plate. Unlike the Kachanov method which neglects the interaction of the tractions of the non-uniform components, the tractions of the non-uniform components on the surfaces of cracks are considered through the alternating technique. The accuracy and efficiency of present method are validated by comparing the results of two collinear and two parallel overlapped open the cracks obtained by the present method with those of the exact solutions, the results of the Kachanov method and the alternating iteration technique. Applications of present method in solving sliding close crack problems and evaluating the plastic zones demonstrate the versatility of present method. 相似文献
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Pongaglabol [8-hydroxy-5-phenyl-furo[2,3-h]benzo(b)pyran-7-one] was synthesized and tested for antibacterial effects againstShigella dysenteriae, Salmonella typhi, Streptococcus β-haemolyticus andStaphylococcus aureus. The synthesized compounds were characterized using UV, IR and1H NMR spectral data 相似文献
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利用电泳技术在不锈钢和铁基底上制备MgB2带材 总被引:2,自引:0,他引:2
本文首次报道了利用电泳技术在铁和不锈钢基底上制备MgB2超导带材.电阻测量表明,沉积在铁和不锈钢基底上MgB2带材的超导零电阻转变温度分别为37.5K和31K,超导转变宽度分别为0.3K和1K.磁测量表明,(5K,OT)时不锈钢基底上带材的临界电流密度为6×105(A/cm2).X射线衍射谱和扫描电子显微镜图象表明样品结晶良好,晶粒生长致密.本工艺制备MgB2带材时不受系统真空度的限制,生长迅速,成本低廉,并且可以根据不同的需要任意选择基底的形状和大小. 相似文献
28.
I. M. Lavit Nguyen Viet Trung 《Journal of Applied Mechanics and Technical Physics》2008,49(3):491-499
The thermoelastoplastic fracture mechanics problem of a thick-walled cylinder subjected to internal pressure and a nonuniform
temperature field is solved by the method of elastic solutions combined with the finite-element method. The correctness of
the solution is provided by using the Barenblatt crack model, in which the stress and strain fields are regular. The elastoplastic
problem of a cracked cylinder subjected to internal pressure and a nonuniform temperature field are solved. The calculation
results are compared with available data.
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Translated from Prikladnaya Mekhanika i Tekhnicheskaya Fizika, Vol. 49, No. 3, pp. 173–183, May–June, 2008. 相似文献
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This study demonstrates the separation of active ingredients in acne formulations (salicylic acid, cloramphenicol and resorcinol in presence of azulene) by capillary zone electrophoresis. Factors affecting their separations were the buffer pH and concentration, applied voltage, sample preparation, and presence of additives. Optimun results were obtained with a 50 mM sodium tetraborate-50 mM sodium phosphate, pH 9.0. The carrier electrolyte gave baseline separation with good resolution, short migration times (<6 min), great reproducibility and accuracy. Calibration plots were linear over at least three orders of magnitude of analyte concentrations, the lower limits of detection being within the range 0.39-1.25 μg ml−1. The procedure was fast and reliable and commercial pharmaceuticals could be analysed without prior sample clean-up procedure. 相似文献
30.
Determination of contact stresses in problems on bending of a package of transversely isotropic bars
V. I. Zubko 《Mechanics of Composite Materials》2004,40(6):495-508
A mechanomathematical model for bending of packages of transversely isotropic bars of rectangular cross section is proposed. Adhesion, slippage, and separation zones between the bars are considered. The resolving equations for deflections and tangential displacements are supplemented with a system of linear differential equations for determining the normal and tangential contact stresses, and boundary conditions are formulated. A scheme for analytical solution of two contact problems—a package under the action of a distributed load and a round stamp—is considered. For these packages, a transition is performed from the initial system of differential equations for determining the contact stresses, where the unknown functions are interrelated by recurrent relationships, to one linear differential equation of fourth order and then to a system of linear algebraic equations. This transition allows us to integrate the initial system and get expressions for the contact stresses.Translated from Mekhanika Kompozitnykh Materialov, Vol. 40, No. 6, pp. 761–778, November–December, 2004. 相似文献