青海省唐古特铁线莲中齐墩果酸含量的测定

建立了唐古特铁线莲中齐墩果酸含量测定的HPLC方法。色谱条件:采用Phenomenex Luna C18(250 mm×4.6 mm,5μm),流动相:乙腈-0.2%H3PO4(35:65),流速:1 mL/min,柱温:室温(25℃),检测波长:205 nm。齐墩果酸在0.0233～0.7000 mg范围内呈良好的线性关系,线性回归方程为y=2E+06x+360803,R2=0.9917,回收率RSD为1.7%,并对青海省野生和栽培唐古特铁线莲中齐墩果酸进行了定量分析,结果显示前者平均值为0.1184%,后者平均值为0.0651%。方法可用以测定唐古特铁线莲中齐墩果酸的含量。     

A new triterpenoid saponin from Clematis ganpiniana

A new triterpenoid saponin,named clematiganoside A(1),was isolated from the whole plant of Clematis ganpiniana.Its structure was elucidated on the basis of 1D,2D NMR,TOF-MS and ESI-MS techniques,and physicochemical properties.     

Two New Steroidal Glycosides from Caryopteris terniflora

Two new steroidal glycosides were isolated from the Chinese medicinal plant Caryopteris terniflora. The spectroscopic and chemical evidences revealed that their structures tobe 6-(β-sitosteroyl-3-O-β-glucopyranosidyl) hexacosanate 1 and 6‘-(stigmasteroyl-3-O-β-glucopy-ranosidyl) linolenate 2, respectively.     

青海不同产地唐古特铁线莲中的5种元素分析比较

为了研究青海不同产地唐古特铁线莲5种元素的分布情况,本研究应用火焰原子吸收光谱法,石墨炉原子吸收光谱法以及分光光度法测定了青海产唐古特铁线莲中5种元素P,K,Mg,Pb,Cd的含量.结果表明,在青海省18个不同产地中,产于阿柔乡1的唐古特铁线莲P和K含量均最高,分别达到4813.19mg/kg和33304.34mg/kg;产于西宁市的唐古特铁线莲中Mg含量最高,为5377.71mg/kg.产于都兰的唐古特铁线莲中Pb含量最高,为10.79mg/kg.产于平安青砂山的唐古特铁线莲中Cd含量最高,为11.04mg/kg.本方法准确可靠,为唐古特铁线莲的进一步研究奠定理论基础.     

青海不同地区唐古特铁线莲中的微量元素分析

采用空气-乙炔火焰原子吸收光谱法对唐吉特铁线莲中的Ca、Zn、Mn、Cu、Fe、Mg、Co7种微量元素进行了含量测定.该方法的加标回收率在98.73％-102.27％之间,相对标准偏差在1.12％-2.10％之间,具有较高的准确度和精确度.唐古特铁线莲中微量元素的含量顺序为Mg＞Ca＞Fe＞Mn＞Zn＞Cu＞Co,且这7种元素与海拔没有线性关系.Mg、Ca、Fe、Mn和Zn含量较为丰富,其平均值分别为273.64、242.85、96.29、4.51、2.68mg/100g.这些研究结果为进一步探讨唐古特铁线莲的药理毒理和药效提供了科学依据.     

青海省唐古特铁线莲质量控制标准的研究

目的对青海省唐古特铁线莲质量控制标准进行研究。方法采用薄层色谱法对唐古特铁线莲进行定性鉴定,展开剂为氯仿与丙酮（95：5）,显色剂为10％硫酸甲醇;采用HPLC（Lc-2010HT）法测定唐古特铁线莲中齐墩果酸的含量,色谱柱为PhenomenexLunaCt8（250mm×4．6mm,5Ixm）,流动相为y（乙腈）＋V（0．2％磷酸水溶液）=35＋65,流量为1mL／min,柱温为室温（25℃）,检测波长为205nm。结果薄层色谱鉴定中,供试品色谱与对照品色谱相应的位置上,显示出相同颜色的荧光斑点;齐墩果酸在0．0233—0,7000mg／mL范围内呈良好的线性关系,线性回归方程为Y=2×10-6＋360803,r。=0．9917,RSD为1．61％,并对青海省野生和栽培唐古特铁线莲中齐墩果酸进行了定量分析,结果显示前者平均值为0．1184％、RSD为2．5113％,后者平均值为0．0651％、RSD为2．92％。结论该法操作简单、快速、准确、灵敏、重现性好,可有效控制青海省唐古特铁线莲的质量。     

Screening of bioconstituents and in vitro cytotoxicity of Clematis gouriana leaves

Clematis gouriana (Ranunculaceae), a perennial herb, is used by the local inhabitants of the western Himalayan region for its medicinal properties. Major bioconstituents of C. gouriana leaves using different solvent extracts were obtained and analysed. The results revealed promising contents of phenolics (from 18.19 ± 0.10 to 22.17 ± 0.10 mg g^? 1) as gallic acid and flavonoids (from 2.83 ± 0.01 to 6.52 ± 0.08 mg g^? 1) as quercetin equivalent in different extracts. Aqueous acetone extract showed higher antioxidant activity with IC₅₀ value of 129.11 and 25.35 μg mL^? 1 against DPPH and ABTS free radicals, respectively. Antioxidant yield ranged from 16.87 ± 0.27 to 24.48 ± 0.13 mg g^? 1 of Trolox equivalent in different extracts as measured by the FRAP assay. Furthermore, ethylacetate extract exhibited strong in vitro cytotoxicity against Chinese hamster ovary and glioma cell lines. Proximate composition (proteins, fats, ash and minerals) of C. gouriana leaves was also assessed. Results demonstrated the potential of C. gouriana bioconstituents as nutraceuticals.     

Macrocyclic Glycosides from Clematis hexapetala

Two new macrocyclic glycosides, clemahexapetoside A ( 1 ) and B ( 2 ), along with the known compound clemochinenoside A ( 3 ), were isolated from the roots and rhizomes of Clematis hexapetala. Their structures were elucidated on the basis of chemical, physicochemical, and spectroscopic evidence.