Capillary Zone Electrophoresis of Eleven Priority Phenols with Amperometric Detection

A scheme for separation and detection of eleven priority phenols using capillary zone electrophoresis (CZE) coupled with amperometric detection is described. With a capillary of I.D. 50 μm and length 62.5 cm at 9 kV and an electrophoretic buffer of 20 mM CHES (pH 10.1), complete separation of the eleven compounds was achieved in less than 17 min. Amperometric detection was carried out using a carbon fiber microelectrode of diameter 9 μm inserted into the end of the detection capillary. Linearity over two orders of magnitude was generally obtained for the eleven priority phenols. With an electrode potential+1.10 V (vs. Ag/AgCl reference), the concentration limits of detection were in the sub-ppm (10^?6 M) level. This method was successfully applied to analysis of priority phenols in industrial waste water.     

毛细管电泳在手性分离中的应用及进展

本文评述了近年来毛细管电泳在手性分离中的应用及进展，介绍了毛细管电泳分离手性对映射的数学模型、五种不同的分离模式及机理、七种常用的手性选择性类型及其在药学、环境和生命科学中的应用、研究中需优化的操作参数及其发展方向。     

毛细管电泳-激光干涉检测法测定饮料中蔗糖含量

本文将毛细管电泳-激光干涉检测法用于饮料中蔗糖的定量测定.结果表明,在样品浓度低于10mg/mL时,检测信号与样品浓度成正比.样品不需衍生化处理直接进行测定,分析结果较为满意.     

毛细管电泳安培检测扑热息痛及其水解物

研究了各种电化学预处理条件下碳纤维电极对扑热息痛及水解物的电化学行为。确定该体系最佳预处理条件为０．２Ｖ电位下阳极化１ｍｉｎ，再于－２．０Ｖ下阴极化１０ｓ。预处理后的碳纤维伏安响应得到明显提高。运用最佳条件并在支持电解中加入添加剂后，扑热息痛及其水解物在毛细管电泳上获得很好的分离和检测。其中扑热息痛的检测下限为２．７８ｐｇ；对氨基酚为１．８４ｐｇ。     

聚丙烯酰胺双向凝胶电泳法分析变色人发角蛋白组成

报道了一种用双向聚丙烯酰胺凝胶电泳分析经Ｓ－羧甲基化修饰的变色人发角蛋白样品的方法，以含８ｍｏ１／Ｌ脲变性凝胶电泳做第一向，ＳＤＳ－ＰＡＧＥ做第二向。不同处理的变色人发得到有差别的双向电泳图谱，即不同处理方法得到的人发蛋白质组成有差异。以此可做为人发变色处理的一个定性方法。     

Capillary Electrophoretic Identification of Commercial Gelling Agents

Gelling agents based on polysaccharides have become extremely important in the food industry. As more and more products like soups and sauces have become available in lyophilized form, thickeners have to be added to give a controlled consistency to the finished meal. A capillary electrophoretic method has been developed to investigate the monosaccharide composition of hydrolyzed thickeners. 2-Aminoanthracene was used as a derivatization reagent to allow sensitive fluorescence detection. This system was applied to a set of standard thickeners as well as to food samples.     

毛细管电泳质谱联用技术及其应用

本文介绍了用于毛细管电泳质谱联用仪器的多种接口技术，描述了ＣＺＥ，ＣＩＥＦ，ＣＧＥ，ＭＥＫＣ和ＣＩＴＰ等毛细管电泳技术和四极质谱，离子阱质谱，傅 叶变换离子回旋共振质谱，飞行时间质谱，磁质谱，解吸质谱等联用的现状及发展前景，对近年来ＣＥ－ＭＳ在酶解产物。蛋白质和肽，核苷酸，药物及代谢产物等领域中的应用作了详细述评。     

不同形态阴离子的高效毛细管电泳的间接紫外检测

本文研究了用毛细管电泳紫外间接检测法对几种阴离子不同形态进行分析。     

ICP-MS for elemental speciation studies

The use of inductively coupled plasma mass spectrometry (ICP-MS) coupled with separation techniques for the purpose of elemental speciation has recently gained a lot of attention. Much of this is due to ever improving separation capabilities of Chromatographic techniques, the high sensitivity of ICP-MS, and the continuing development of better interface techniques. Additionally, there is a growing awareness of the need to monitor various species of an analyte, rather than just total analyte concentrations, due to their often varying natures. For the sake of learning from different elemental speciation approaches, this review brings together some selected types of elemental speciation which have been recently seen in literature. These include separations using various forms of liquid chromatography, such as reversed phase, reversed phase ion pairing, micelle, ion exchange, and size exclusion. Elemental speciation employing gas Chromatographie separations and supercritical fluid separations are discussed as well as elemental speciation using capillary electrophoresis.