Microfluidic chips for mass spectrometry‐based proteomics

Microfluidic devices coupled to mass spectrometers have emerged as excellent tools for solving the complex analytical challenges associated with the field of proteomics. Current proteome identification procedures are accomplished through a series of steps that require many hours of labor‐intensive work. Microfluidics can play an important role in proteomic sample preparation steps prior to mass spectral identification such as sample cleanup, digestion, and separations due to its ability to handle small sample quantities with the potential for high‐throughput parallel analysis. To utilize microfluidic devices for proteomic analysis, an efficient interface between the microchip and the mass spectrometer is required. This tutorial provides an overview of the technologies and applications of microfluidic chips coupled to mass spectrometry for proteome analysis. Various approaches for combining microfluidic devices with electrospray ionization (ESI) and matrix‐assisted laser desorption/ionization (MALDI) are summarized and applications of chip‐based separations and digestion technologies to proteomic analysis are presented. Copyright © 2009 John Wiley & Sons, Ltd.     

Multiplexed p53 Mutation Detection by Microchip Electrophoresis with Laser-Induced Fluorescence Detector

A form of single‐strand DNA‐conformation polymorphism analysis (SSCP) employing nondenaturing slab gel electrophoresis is applicable to the genetic diagnosis of mutations at exons 7, 8 and 9 of the p53 gene. Recently, microchip electrophoresis (ME) systems have been used in SSCP analysis instead of conventional slab gel electrophoresis in terms of speed, sensitivity and automation. The aim of the present study was to investigate the application of SSCP and ME analysis as a rapid and effective method to the detection of mutations for exons 7, 8 and 9 of the p53 gene. It was found that using the electric field strength 260 V/cm and the sieving matrix of 4 mg/mL poly(ethylene oxide) was very useful to achieve better resolution and fast detection of mutations at exons 7, 8 and 9 of p53 gene. Under the optimized conditions, mutations at exons 7–9 of p53 gene were analyzed within 60 s and the relative standard deviation values of the migration times were less than 5.81% (n=5). The detection limit can be as low as 1 ng·L^?1.     

激光反射镜与诱导透射滤光片工艺分析

随着光通信和光信息技术的迅速发展,介质高反射镜和窄带滤光片倍受人们关注,有机光学薄膜滤波器材料及器件将日益受到重视.采用一个低通滤波器和高通滤波器在其通带叠合处便可产生一个优良的窄带滤波器.详细介绍了几种光学薄膜器件激光反射镜、诱导透射滤光片(ITF)和微片式激光器的混合材料及制备工艺,并给出其设计方法,对批量生产将有极大的促进作用.     

Electrical field-induced extraction and separation techniques: Promising trends in analytical chemistry – A review

Sample preparation is an important issue in analytical chemistry, and is often a bottleneck in chemical analysis. So, the major incentive for the recent research has been to attain faster, simpler, less expensive, and more environmentally friendly sample preparation methods. The use of auxiliary energies, such as heat, ultrasound, and microwave, is one of the strategies that have been employed in sample preparation to reach the above purposes. Application of electrical driving force is the current state-of-the-art, which presents new possibilities for simplifying and shortening the sample preparation process as well as enhancing its selectivity. The electrical driving force has scarcely been utilized in comparison with other auxiliary energies. In this review, the different roles of electrical driving force (as a powerful auxiliary energy) in various extraction techniques, including liquid-, solid-, and membrane-based methods, have been taken into consideration. Also, the references have been made available, relevant to the developments in separation techniques and Lab-on-a-Chip (LOC) systems. All aspects of electrical driving force in extraction and separation methods are too specific to be treated in this contribution. However, the main aim of this review is to provide a brief knowledge about the different fields of analytical chemistry, with an emphasis on the latest efforts put into the electrically assisted membrane-based sample preparation systems. The advantages and disadvantages of these approaches as well as the new achievements in these areas have been discussed, which might be helpful for further progress in the future.     

二极管泵浦双掺杂YAG微片自调Q激光器

 利用激光二极管泵浦双掺杂YAG微片晶体，在连续泵浦时获得了重复频率为2.78kHz，脉冲宽度为6ns的自调Q激光输出。并通过预泵浦加脉冲调制电流的方法，实现可控重复率的自调Q脉冲输出。与外腔式自调Q微片激光器相比，这种一体化微片激光器具有更高的转换效率、更窄的输出脉冲宽度和更高的重复频率，同时结构更为简单紧凑，易于调整。     

A simple and compact blue diode laser powered excitation source for fluorescence detection in capillary electrochromatographic microchip separation

A simple and compact fluorescence excitation source was prepared using a 405 nm blue laser diode module and characterized in capillary electrochromatographic or capillary electrophoretic microchip separation. An inexpensive blue laser diode module with a tiny focusing lens was simply mounted at the center of an aluminum block on a miniature linear motion guide for heat dissipation and position control. A slit unit has a series of fifteen laser-machined slits with 1 mm space along the direction of the separation channel of the microchip above this unit. The laser beam was focused through a slit with 50 μm width to the separation channel at the position of a desired length. Although the excitation source unit was connected to a simple current controlled power supply, it was stable with 0.1% drift per hour and 1.3% (1σ) fluctuation in intensity. This simple excitation source can be prepared easily with inexpensive minimum optical components and mounted with a microchip on the stage of an ordinary fluorescence microscope for daily separation studies using a CE or CEC microchip. The applicability of the excitation source was evaluated with FITC-amino acid derivative mixtures using a polymer based CEC microchip packed fully with submicron silica beads in its microchannel.     

Performance Analysis of a New Water-based Microcooling System

In the electronic industry, dissipating the heat load becomes a critical factor for highly developed designs. These require higher power transfer in a more compact size. In the current study, a new microcooling system was developed and tested. It utilizes the enhancement in heat transfer characteristics associated with implementing a vortex promoter in the evaporator segment of a water-based heat pipe. The test evaporator was a cavity of 4-mm diameter and 23-mm length in an electrically heated aluminum block. A helical coil (of various diameters, namely 500, 300, and 250 μm) was introduced to the evaporator segment to act as a vortex promoter. Configurations of a new microcooling system based on a modified heat pipe technology were built and tested. The presented system proves to work efficiently in situations where a closed-loop thermosyphon encounters film boiling limitation. The most efficient configuration has a flow modifier diameter about one-tenth of the evaporator chamber gap, while the diameter of the return line was three-quarters of the evaporator gap. This configuration shows a stable operation characteristic and possesses high thermal efficiency. The maximum heat flux obtained by such a configuration was 305 W/cm² when it runs at 103°C saturated temperature and 0.01°C/W thermal resistance. A uniform temperature distribution along the system was noticed.     

全固态被动调Q皮秒激光技术研究进展

全固态被动调Q激光技术在产生皮秒脉冲方面有较快发展。与锁模激光器相比,被动调Q皮秒激光器成本低、结构简单、易于校准光路,避免了锁模激光器结构复杂、机械敏感度高、光路校准困难等缺点,并且同样能够输出单脉冲能量可观、重频合适的皮秒量级短脉冲,因此拥有较高的实用价值。本文讨论了全固态被动调Q皮秒激光技术领域的两种典型技术路线以及对调Q皮秒脉冲输出的后续处理技术,包括非线性技术和激光放大技术等,并介绍了国内外相关研究团队在该领域所做的工作及其突破性进展。     

消息驱动思想在单片机定时体系中的应用

提出了用消息驱动思想来构造单片机控制系统中的定时体系，阐述了消息驱动思想的基本原理及其在单片机系统中的具体实现．该思想方便地解决了用较少资源实现复杂多变的定时要求的问题．     

微流控进样阿达玛变换显微荧光成像单细胞分析

将微流控芯片用于单细胞进样,以自制阿达玛变换显微荧光成像分析系统进行成像检测,讨论了影响单细胞进样和荧光成像的主要因素,并对花粉细胞DNA含量进行定量分析。结果表明：微流控进样技术与HT显微成像技术相结合,可有效可靠地应用于单细胞分析中,所测定的三个玉帘花粉的DNA含量分别为124．4、123．9和62．9pg。