人工选育F5代萍乡肉红鲫RAPD遗传分析

萍乡肉红鲫是分布于江西萍乡的具有地方特色的品种,经过人工选育获得了较为稳定的遗传特征。用经过筛选的23个随机引物对25个人工选育F5代萍乡肉红鲫基因组DNA进行RAPD分子标记。结果显示扩增片段大小在250 bp至2 100 bp,共扩增的条带数为229,差异标记条带大多数为弱带,多态位     

Seed Oils of Fifteen Ebenus Taxa Growing in Turkey

Oil yields and compositions from seeds of 15Ebenustaxa growing in Turkey were investigated. The yields were found between 4.0% and 13.0%. The seeds ofE. Barbigerashowed the highest whileE. Plumosavar.plumosashowed the lowest yield of oil among the 15 taxa investigated. GC/MS showed that linoleic acid (42.8-55.6%), palmitic acid (13.8-23.6%), and oleic acid (15.9-23.6%) are the main fatty acid components of all the species     

高速逆流色谱法分离纯化黄芪中的芒柄花素和毛蕊异黄酮

采用高速逆流色谱法(HSCCC),以正己烷-氯仿-甲醇-水组成二相系统作为固定相与流动相,对黄芪的乙酸乙酯粗提 物进行了分离纯化。 结果发现:以正己烷-氯仿-甲醇-水(体积比为1.5∶3∶3∶2)组成的系统可以从黄芪的乙酸乙酯粗 提物中分离出毛蕊异黄酮,纯度可达95％以上,并可以初步纯化芒柄花素;接着用正己烷-氯仿-甲醇-水(体积比为4∶4∶5 ∶4)组成的系统进一步纯化芒柄花素,其纯度达95％以上。利用该方法,可以对中药黄芪中的异黄酮进行快速的分离和纯 化。     

Secoiridoids Glycosides from Some Selected Jasminum SPP

Secoiridoid glycosides of three oleaceaous plants are described. Fractionation of the ethanolic extracts of Jasminum nitidum Skan, J. giraldii Diels and J. sambac (L.) Ait. var. trifoliatum Hort. (Oleaceace) resulted in the isolation of syringalactones A ( 1 ) and B ( 2 ), oleoside 7,11‐dimethyl ester ( 3 ), jasminoside (7), jaslanceosides B ( 8 ), sambacoside A ( 9 ) and molihuaside D ( 10 ). The structures of these secoiridoid glycosides were elucidated on the basis of spectral methods. Chemotaxonomy for plants of Jasminum is also discussed.     

The Low Polar Constituents from Bidens Pilosa L. var. Minor (Blume) Sherff

Anew and 20 known compounds were isolated from Bidens pilosa L. var. minor (Blume) Sherff. The new compound was determined as 7‐phenyl‐hepta‐4,6‐diyne‐2‐ol by various physical techniques (MS, IR, UV, ¹H‐, ¹³C‐NMR, and 2D‐NMR).     

Spiramide,a New Diterpene Amide from the Roots of Spiraea japonica var.acuta

Previous chemical investigations on SPiraea j'aponica and its varieties have led to thereport of 7 new atisane-type diterpenoids' and 30 new diterpene alkaloids of atisine- andhetisine-ace'. The chemotaxonomy of S. japonica complex was proposed based onthose chemical data3. A hypothetical pathway of the biosynthesis of atisine-typealkaloids was suggested to be formed from diterpene directly tO the correspoedngalkaloids through double Mannich reactions (unpublished data). In order to search f…     

锦灯笼中无机元素含量测定

采用ＩＣＰ－ＡＥＳ法检测了要笼果实及其干燥宿萼中无机元素含量。结果表明,锦灯笼含有丰富的对人体有益的无机元素,具较高的营养价值。     

Quantitative determination and evaluation of Paris polyphylla var. yunnanensis with different harvesting times using UPLC‐UV‐MS and FT‐IR spectroscopy in combination with partial least squares discriminant analysis

A rapid method was developed and validated by ultra‐performance liquid chromatography–triple quadrupole mass spectroscopy with ultraviolet detection (UPLC‐UV‐MS) for simultaneous determination of paris saponin I, paris saponin II, paris saponin VI and paris saponin VII. Partial least squares discriminant analysis (PLS‐DA) based on UPLC and Fourier transform infrared (FT‐IR) spectroscopy was employed to evaluate Paris polyphylla var. yunnanensis (PPY) at different harvesting times. Quantitative determination implied that the various contents of bioactive compounds with different harvesting times may lead to different pharmacological effects; the average content of total saponins for PPY harvested at 8 years was higher than that from other samples. The PLS‐DA of FT‐IR spectra had a better performance than that of UPLC for discrimination of PPY from different harvesting times.     

Biomonitoring of traffic originated PAHs in the air

This article sets out the results of air quality biomonitoring along a transect bordering on the A32 Turin–Bardonecchia motorway (Italy). The results were obtained using aeroponic sampling units, an innovation insofar as the plants acting as monitor live and grow in these units above ground, assuring that atmospheric pollutants are absorbed only through the leaves. The accumulation of 15 polynuclear aromatic hydrocarbons (PAHs) on the leaves of Brassica oleracea var. Acephala, used as biosampler, was determined on samples taken in the months of February, July and October 2002, in each case after two months' exposure. The results highlight: (i) the influence of the seasons on the accumulation of pollutants, (ii) that phenanthrene, fluoranthene, pyrene and chrysene generally account for more than 80% of total PAHs, (iii) that the data may be considered representative of both volatile and non-volatile compounds. Comparison with other sampling methods are supplied and discussed.     

Neomerane-type sesquiterpenoids from Valeriana officinalis var. latifolia

Valeneomerins A–D, two new neomerane-type sesquiterpenoids and two new nor-sesquiterpenoids, were isolated from the roots of Valeriana officinalis var. latifolia. Among them, valeneomerin A (1) was identified as a sesquiterpenoid with an unprecedented 15,3-lactone loop while valeneomerin C (3) and valeneomerin D (4) were determined to be nor-sesquiterpenoids with a new 15-reductive neomerane-type carbon skeleton. Their structures were elucidated by detailed spectroscopic analysis and Cu Kα X-ray crystallography. Compounds 1, 2, and 4 exhibited significant neuroprotective effects against H₂O₂ induced oxidative stress in SH-SY5Y cells with viability of 49.8, 40.9, and 44.6% each at 50 μM, respectively, in contrast to positive control (catechin, 50 μM) with viability of 32.6%.