排序方式: 共有132条查询结果,搜索用时 15 毫秒
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Dr. Haibin Xiao Dr. Wen Zhang Prof. Ping Li Prof. Wei Zhang Xin Wang Prof. Bo Tang 《Angewandte Chemie (Weinheim an der Bergstrasse, Germany)》2020,132(11):4244-4258
The superoxide anion (O2.−) is widely engaged in the regulation of cell functions and is thereby intimately associated with the onset and progression of many diseases. To ascertain the pathological roles of O2.− in related diseases, developing effective methods for monitoring O2.− in biological systems is essential. Fluorescence imaging is a powerful tool for monitoring bioactive molecules in cells and in vivo owing to its high sensitivity and high temporal-spatial resolution. Therefore, increasing numbers of fluorescent imaging probes have been constructed to monitor O2.− inside live cells and small animals. In this minireview, we summarize the methods for design and application of O2.−-responsive fluorescent probes. Moreover, we present the challenges for detecting O2.− and suggestions for constructing new fluorescent probes that can indicate the production sites and concentration changes in O2.− as well as O2.−-associated active molecules in living cells and in vivo. 相似文献
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Dr. Koji Numasawa Prof. Kenjiro Hanaoka Naoko Saito Prof. Yoshifumi Yamaguchi Takayuki Ikeno Honami Echizen Dr. Masahiro Yasunaga Dr. Toru Komatsu Dr. Tasuku Ueno Prof. Masayuki Miura Prof. Tetsuo Nagano Prof. Yasuteru Urano 《Angewandte Chemie (Weinheim an der Bergstrasse, Germany)》2020,132(15):6071-6076
Folate receptors (FRs) are membrane proteins involved in folic acid uptake, and the alpha isoform (FR-α) is overexpressed in ovarian and endometrial cancer cells. For fluorescence imaging of FRs in vivo, the near-infrared (NIR) region (650–900 nm), in which tissue penetration is high and autofluorescence is low, is optimal, but existing NIR fluorescent probes targeting FR-α show high non-specific tissue adsorption, and require prolonged washout to visualize tumors. We have designed and synthesized a new NIR fluorescent probe, FolateSiR-1 , utilizing a Si-rhodamine fluorophore having a carboxy group at the benzene moiety, coupled to a folate ligand moiety through a negatively charged tripeptide linker. This probe exhibits very low background fluorescence and afforded a tumor-to-background ratio (TBR) of up to 83 in FR-expressing tumor-bearing mice within 30 min. Thus, FolateSiR-1 has the potential to contribute to the research in the field of biology and the clinical medicine. 相似文献
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Haw‐Young Kwon Xiao Liu Eun Gyeong Choi Jung Yeol Lee So‐Young Choi Jun‐Young Kim Lu Wang Sung‐Jin Park Beomsue Kim Yong‐An Lee Jong‐Jin Kim Nam Young Kang Young‐Tae Chang 《Angewandte Chemie (Weinheim an der Bergstrasse, Germany)》2019,131(25):8514-8519
The rapid and sensitive classification of bacteria is the first step of bacterial community research and the treatment of infection. Herein, a fluorescent probe BacGO is presented, which shows the best universal selectivity for Gram‐positive bacteria among known probes with a minimum staining procedure for sample detection and enrichment of the live bacteria. BacGO could also be used to assess of the Gram status in the bacterial community from wastewater sludge. Furthermore, BacGO could sensitively and selectively detect a Gram‐positive bacterial infection, not only in vitro but also using an in vivo keratitis mouse model. BacGO provides an unprecedented research tool for the study of dynamic bacterial communities and for clinical application. 相似文献
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