毛细管电泳迁移时间重现性影响因素的探讨

以电泳介质为研究对象,在毛细管区带电泳(CZE)和胶束电动毛细管色谱(MECC或MEKC)两种电泳模式下,探讨了电泳 介质中各组分浓度、冲洗程序、电泳介质在实验中发生的变化对溶质迁移时间重现性的影响。根据描述缓冲液中各组分 浓度与电渗流迁移时间和溶质迁移时间的关系式,证明利用电导值可准确地表征电泳缓冲液的浓度;通过控制缓冲液的电 导值可提高迁移时间的重现性。运行缓冲液pH值的变化影响毛细管壁上的硅羟基的电离,因此需选择合理的冲洗程序使硅 羟基的电离达到平衡,以提高迁移时间的重现性。毛细管入口端缓冲液是影响溶质迁移行为的主要因素,其在电泳中发生 的变化是影响电泳重现性的重要原因。目前在实验中可通过提高运行缓冲液的更换频率来保证迁移时间的重现性。     

Electrochemical Cleanup of Solutions for UV-Visible Assays Involving Low Absorbance or Dilute Samples

Abstract

In low-absorbance assays, the necessity for eliminating ultraviolet and visible absorbing impurities in buffering agents and detergents is discussed. The method proposed here involves electrochemical reduction of the interfering material. An example of the elimination of an artifact in an enzyme assay is presented.     

长寿命斯特林制冷机膨胀机无摩擦运行技术

论述了长寿命斯特林制冷机膨胀机的结构,重点对排出器活塞与冷指气缸无接触运行控制方法进行论述.制冷机正在进行加速寿命跑和,运行很好.     

Empirical predictor of conditions that support ideal-filter capillary electrophoresis

Ideal-filter CE (IFCE) is a method for the selection of affinity binders for protein targets from oligonucleotide libraries, for example, random-sequence oligonucleotide libraries and DNA-encoded libraries, in a single step of partitioning. In IFCE, protein–oligonucleotide complexes and unbound oligonucleotides move in the opposite directions, facilitating very high efficiency of their partitioning. For any given protein target and oligonucleotide library, protein–oligonucleotide complexes and unbound oligonucleotides move in the opposite directions only for a limited range of EOF mobilities, which, in turn, corresponds to a limited range of pH and ionic strength values of the running buffer. Rational design of IFCE-based partitioning requires a priori knowledge of this range of pH and ionic strength values, and here we introduce an approach to predict this range for a given type of the running buffer. The approach involves measuring EOF mobilities for a relatively wide range of pH and ionic strength (I) values and finding an empirical predictor function that related the EOF mobility with pH and ionic strength. In this work, we developed a predictor function for a running buffer (Tris-HCl) that is commonly used in CE-based partitioning of affinity binders for protein targets. This predictor function can be immediately used for the rational design of IFCE-based partitioning in this running buffer, while the described approach will be used to develop predictor functions for other types of running buffer if needed.     

Determination of Bromate and Bromide in Seawater by Ion Chromatography, with an Ammonium Salt Solution as Mobile Phase, and Inductively Coupled Plasma Mass Spectrometry

A method has been developed for determination of bromate and bromide in water containing high concentrations of chloride (e.g. seawater). Separation of bromate and bromide on an anion-exchange column was followed by ICP–MS detection. To reduce interference of chloride with determination of bromate and bromide, and to avoid clogging, ammonium salts, for example NH₄H₂PO₄, (NH₄)₂HPO₄, (NH₄)₂CO₃, and NH₄NO₃ were examined as mobile-phase components. It was found that mobile phase containing 20 mM NH₄NO₃ at pH 5.80 was compatible with the anion-exchange column and enabled reasonable resolution and separation of bromate and bromide within 7 min. Detection limits for bromate and bromide ranged from 2.0 to 3.0 μg L⁻¹ for direct injection of 50 μL sample without matrix elimination. The proposed method was used for analysis of bromate and bromide in seawater.     

Isoelectric buffers, part 3: determination of pKa and pI values of diamino sulfate carrier ampholytes by indirect UV-detection capillary electrophoresis

Ampholytes with close pK(a) values (i.e., good carrier ampholytes (CAs)) are needed as buffers in pH-biased isoelectric trapping (IET) separations. The syntheses of two families of such good CAs were reported recently. Members of the family of diamino sulfate ampholytes (first series) had pI values in the 5.7 < pI < 9.0 range. Members of the family of quaternary ammonium dicarboxylic acid ampholytes (second series) had pI values in the pI < 4.3 range. To further characterize the diamino sulfate ampholytes, their effective mobilities were measured by indirect UV-absorbance detection capillary electrophoresis in a series of background electrolytes (BGEs) with different pH values. The pK(a) and limiting ionic mobility values of the CAs were obtained by fitting these mobility values, as a function of the pH and the ionic strength of the BGEs, to the theoretical mobility expression. These diamino sulfates complete the list of CAs suitable for IET separations.     

A family of high-buffering capacity diamino sulfate isoelectric buffers for pH-biased isoelectric trapping separations

pH-biased isoelectric trapping separations are hindered by the lack of suitable isoelectric buffers with pI values in the 5.8 < pI range. Two generic approaches are described here for the cost-effective synthesis of a family of diamino sulfate buffers that have high buffering capacities in their isoelectric state: the first approach relies on the sulfation of existing, commercially available diamino alcohol intermediates, the second approach calls for the synthesis of diamino alcohols from epichlorohydrin and widely available secondary amines, and subsequent sulfation of the new diamino alcohol. The diamino sulfate buffers are recovered in isoelectric state, in high purity. Four members of the family having pI values in the 5.8 < pI < 8.9 range have been synthesized, analytically characterized by capillary electrophoresis (CE), electrospray ionization-time of flight-mass spectrometry (ESI-TOF-MS), 1-D and 2-D nuclear magnetic resonance (NMR) spectroscopy, and X-ray crystallography. All four diamino sulfates have been successfully used as pH biasers in the receiving stream in preparative-scale pH-biased isoelectric trapping protein separations.     

Splitting methods for Hamilton‐Jacobi equations

We explain how the exploitation of several kinds of operator splitting methods, both local and global in time, lead to simple numerical schemes approximating the solution of nonlinear Hamilton‐Jacobi equations. We review the existing local methods which have been used since the early 80's and we introduce a new method which is global in time. We show some numerical experiments. © 2005 Wiley Periodicals, Inc. Numer Methods Partial Differential Eq, 2006     

A QoS-aware wavelength assignment scheme for optical networks

Dispersion in fiber optic is wavelength dependent and it degrades the quality of service (QoS) in an optical network. Although use of dispersion compensating fiber reduces the effects of dispersion but it is very costly. In this paper, we propose a QoS-aware wavelength assignment (QWA) scheme to improve the quality of service in an optical network by reducing the overall dispersion in the network. In this scheme, the connection requests with longer lightpath are assigned the wavelengths having lesser dispersion and the wavelengths having higher dispersion are assigned to the lightpaths with shorter distance. The lightpaths are computed using alternate path routing to achieve the lower blocking probability. The performance analysis of QWA scheme is done in terms of total dispersion using step-index fiber (SIF). Results of our experiments show that QWA scheme outperforms conventional wavelength assignment scheme based on First-Fit method (WAFF).     

Performance improvement for N × 80-Gb/s WDM transmission link with optimized alternate polarization

In this paper, it is shown that at a high bit rate of 80-Gb/s alternate polarization of adjacent bits in a Wavelength Division Multiplexed (WDM) transmission link improves the system performance in terms of improved Q factor and minimum bit error rate (BER). Alternate Polarization Return to Zero (al-PRZ) further suppresses the non-linear effects at higher power levels of 25 dBm per channel and also improves the transmission length to 640 km for a N × 80-Gb/s WDM system and hence results in an improvement of BER to 10⁻²⁰.