铁-硫氰酸盐-结晶紫体系-离心光度法测定血清中铁

本文提出了高灵敏离心光度法测定血清铁的新方法。在Fe－SCN－CV体系中，λmax625nm处的表观摩尔吸光系数ε＝1．7X106　L·mol-1·cm-1，Fe（Ⅲ）量在20～160μg／L范围内呈线性关系。将此法用于人体血清铁的临床检验，结果满意。     

铬(Ⅵ)-二溴茜素紫-OP体系显色反应的研究及其在金属材料分析中的应用

本文研究了在HCl与H_3pO_4的混合酸介质中,乳化剂OP存在下,铬(Ⅵ)与2.7-二溴茜素紫(DBV)的显色反应。配合物组成Cr(Ⅵ):DBV=1:3,表观摩尔吸光系数为2.74×10~4L·mol~(-1).cm~(-1)(λmax=416nm)。反应的选择性高,常见金属离子均不干扰铬(Ⅵ)的测定,拟定了用二溴茜素紫分光光度法测定金属材料中铬的方法。     

溴酸钾氧化茜素绿催化光度法测定痕量亚硝酸根

基于酸性介质溴酸钾氧化茜紊绿反应,提出了测定痕量亚硝酸根新的催化光度法.本法的Sandell灵敏度为9.4×10~(-5)μg/cm~2亚硝酸态氮,测定亚硝酸根量的线性范围为0.01～0.12μg/ml,可用于水样中亚硝酸根测定。     

乙基紫标记分光光度法测定脱氧核糖核酸

研究了阳离子染料乙基紫与脱氧核糖核酸的结合反应，在ｐｈ＝６．４－７．４的条件下，乙基紫在５９５ｎｍ处有最大吸收峰，当加入ＤＮＡ后，乙基紫的吸收峰显著下降，而在５０７ｎｍ处出现新的吸收峰。以乙基紫为标记物，根据其５９５ｎｍ吸收峰下降的程度，可用于ＤＮＡ的定量测定。     

基于亚硝化反应的极谱法测定亚硝酸根的研究

提出了一种基于在酸性介质中甲基紫与亚硝酸根发生亚硝化反应，极谱法测定亚硝酸根的新体系。亚硝化产物在－０．７５Ｖ（ｖｓ．ＳＣＥ）处２．５次微分波的波高与ＮＯ２－的质量浓度在２．０×１０－３～２．０×１０－２ｍｇ／Ｌ（Ｓ＝４μＡ），４．０×１０－３～１．６×１０－１ｍｇ／Ｌ（Ｓ＝０．０２ｍＡ）范围内成直线关系。该法重复性好，且大多数常见的阴、阳离子不干扰，用于污水中痕量ＮＯ２－的测定，结果满意。     

结晶紫的表面增强共振拉曼光谱和银胶浓度的关系

常用的银胶一般浓度较低且不稳定,这就限制了它在表面增强共振拉曼谱(SERRS)中的应用,本文旨在探讨一种容易制备,浓度高而且特别稳定的银胶在增强结晶紫(CV)SERRS讯号方面的应用,对一定浓度的CV,我们发现只有配合适当浓度的银胶才能获得最强的SERRS的信号,这个现象是由于稳定剂(PVP)在银颗粒表面的作用造成的。     

A surface-enhanced Raman scattering method for detection of trace glutathione on the basis of immobilized silver nanoparticles and crystal violet probe

Unsatisfactory sensitivity and stability for molecules with low polarizability is still a problem limiting the practical applications of surface-enhanced Raman scattering (SERS) technique. By preparing immobilized silver nanoparticles (Fe₃O₄/Ag) through depositing silver on the surface of magnetite particles, a highly sensitive and selective SERS method for the detection of trace glutathione (GSH) was proposed on the basis of a system of Fe₃O₄/Ag nanoparticles and crystal violet (CV), in which the target GSH competed with the CV probe for the adsorption on the Fe₃O₄/Ag nanoparticles. Raman insensitive GSH replaced the highly Raman sensitive CV adsorbed on the surface of Fe₃O₄/Ag particles. This replacement led to a strong decrease of the CV SERS signal, which was used to determine the concentration of GSH. Under optimal conditions, a linear response was established between the intensity decrease of the CV SERS signal and the GSH concentration in the range of 50–700 nmol L⁻¹ with a detection limit of 40 nmol L⁻¹. The use of a Fe₃O₄/Ag substrate provided not only a great SERS enhancement but also a good stability, which guarantees the reproducibility of the proposed method. Its use for the determination of GSH in practical blood samples and cell extract yielded satisfactory results.     

Analysis of questioned documents: A review

During the last years (2000–2014), many publications concerning the forensic analysis of questioned documents have been published, and new techniques and methodologies are nowadays employed to overcome forensic caseworks. This article reviews a comprehensive collection of the works focused on this issue, including dating studies, the analysis of inks from pens and printers, the analysis of paper, the analysis of other samples related to questioned documents and studies on intersecting lines. These sections highlight the most relevant analytical studies by a wide range of analytical techniques. Separation and spectrometric techniques are critically discussed and compared, emphasizing the advantages and disadvantages of each one. Finally, concluding remarks on the research published are included.     

铜烟灰中铼的测定

研究了在ｐＨ＝ ２．４ 的磷酸氢二钠－ 柠檬酸缓冲体系中， 用苯萃取高铼酸根与乙基紫生成蓝绿色的离子缔合物。 试剂和缔合物的最大吸收均在６１１ ｎｍ 处。 表观摩尔吸光系数 ε＝ ２ ．７６ ×１０５ Ｌ·ｍｏｌ－ １ ·ｃｍ － １ ， 铼含量在０ ～１ ．２ ｍｇ／Ｌ 范围内服从比尔定律。 方法用于电吸尘铜烟灰中铼的测定， 结果令人满意。