Determination of Pongamol and Karanjin in Karanja Oil By Reverse Phase Hplc

ABSTRACT

Pongamol and karanjin, naturally present in karanja (Pongamia glabra) oil possess potential UV A and UV B sunscreen activities, respectively. A reverse phase high performance liquid chromatography (RP-HPLC) method has been developed for direct estimation of these constituents of karanja oil. This method of analysis requires no sample preparation of active ingredients. The analysis uses methanol, water and acetic acid in the ratio of 85: 13.5: 1.5 as mobile phase at a flow rate of 0.5 ml/ min on a Kromasil 100 C18 column, ex Tracer (250mm X46mm, particle size 5 microns) and dual wavelength detection at 350 and 300 nm. 2-ethylhexyl-p-methoxy cinnamate (Parsol MCX) was used as the internal standard. The identical UV spectrum of the chromatographic region (upslope, apex and downslope) confirmed that the analysis was free from substrate interference. The detection limits for pongamol and karanjin were found to be 0.1 μg/ml.     

Active Site Titration of Horse Liver Alcohol Dehydro-Genase by Dual Wavelength Specthophotomethy

Abstract

A simple dual wavelength spectrophotometric method for determining the active site concentration of horse liver alcohol dehydrogenase is described. The method is rapid, sensitive, accurate and requires minute amounts of enzyme. A comparison between this new application of the dual wavelength technique and the conventional methods, ordinary photometry and spectrofluorometry is furthermore discussed.     

HCl-NaCl-C3H8O3-H2O体系溶液热力学性质

在恒定丙三醇质量分数x=0．1的条件下，测定了无液接电池（A）和电池（B）的电动势根据电池（A）电动势确定了丙三醇和水混合溶剂中的Ag－AgCl电极的标准电极电势，讨论了HCl的迁移性质；由电池（B）测得的电动势计算了HCl在该体系中的活度系数γA，计算的结果表明，对于所讨论的体系，在溶液中总离子强度保持恒定，HCl的活度系数服从Harned规则．在溶液组成恒定时，IgγA是温度倒数1／T的线性函数，讨论了混合物中HCl的相对偏摩尔焓及介质效应．     

Computer based screening of compound databases: 1. Preselection of benzamidine-based thrombin inhibitors

We present a computational protocol which uses the known three-dimensional structure of a target enzyme to identify possible ligands from databases of compounds with low molecular weight. This is accomplished by first mapping the essential interactions in the binding site with the program GRID. The resulting regions of favorable interaction between target and ligand are translated into a database query, and with UNITY a flexible 3D database search is performed. The feasibility of this approach is calibrated with thrombin as the target. Our results show that the resulting hit lists are enriched with thrombin inhibitors compared to the total database.     

Liquidus Curves of NaNO3(aq) Calculatedfrom the Modified Adsorption Isotherm Modelfor Aqueous Electrolytes

Summary.  The Stokes-Robinson modification of the Brunauer-Emmett-Teller (BET) adsorption isotherm is used to calculate the liquidus curve of NaNO₃(aq) including the eutectic point and metastable phases. The method described here represents a simplified approach to predict the liquidus curves with sparse information. Received August 9, 1999. Accepted November 4, 1999     

通过快速定点突变表征人源含硒单链抗体酶的底物结合部位

为了对人源含硒单链抗体酶Se-scFv-B3的底物结合部位和催化基团进行研究, 在理论预测的基础上, 通过快速定点突变法分别在2个理论预测的底物结合部位(位点1和位点2)内选定Ala180和Ala44定点突变为丝氨酸(Ser). 2个突变体蛋白经化学修饰将Ser转变成谷胱甘肽过氧化物酶(GPX)的催化基团硒代半胱氨酸(Sec)后, 前者的GPX活力达到了Se-scFv-B3的2倍多, 而后者的GPX活力没有明显提高, 这表明位点1可能是主要的底物结合部位, 与理论预测的结果一致.     

An effective sequence characterized amplified region‐PCR method derived from restriction site‐amplified polymorphism for the identification of female Schistosoma japonicum of zoonotic significance

In the present study, restriction site‐amplified polymorphism (RSAP) markers were used to examine the genetic variability of Schistosoma japonicum isolates from different endemic provinces in mainland China. Of the 45 pairs of primers screened, 10 RSAP markers showed a clear banding pattern with good resolution; however, only six exhibited a polymorphism among different isolates. Among six RSAP markers, one pair of primers (R8+R10) was able to differentiate male and female parasites, and amplified one constant specific band for female S. japonicum isolates. The specific band was recovered, re‐amplified and sequenced, and a sequence of 162 bp was obtained. Based on this sequence, a pair of specific primers was designed and used to develop sequence characterized amplified region (SCAR)‐PCR assay for identification and differentiation of female S. japonicum isolates. The SCAR‐PCR assay allowed the specific identification of female S. japonicum, with no amplicons being amplified from male S. japonicum, Fasciola hepatica, Clonorchis sinensis, S. mansoni (male and female parasite). DNA sequencing confirmed the identity of the amplified products. The minimum amount of DNA detectable using SCAR‐PCR assay was 0.3 ng for female S. japonicum. The SCAR‐PCR was able to differentiate effectively the male and female S. japonicum worms collected from 12 geographical origins in eight endemic provinces, the gender of which was known based on the morphological and biological features. These results showed that SCAR‐PCR provides an effective tool for the sex differentiation studies of S. japonicum, identification of female S. japonicum, diagnosis and epidemiological survey of S. japonicum infections in animals and human.     

一种高活性透明液相NaY沸石导向剂的制备及性能研究

制备了一种摩尔组成为17Na2O:1Al2O3,:17SiO2:300～380H2O的透明液相导向剂.并以天然高岭土为原料,硅溶胶为补加硅源与该导向剂组成反应混合物原位水热合成高硅NaY分子筛.分析了导向剂的结构与作用机理,详细考察了导向剂老化时间、老化温度及其加入量对导向剂的活性与合成产物性能的影响.发现只有在导向剂存在时才能合成出NaY沸石.当导向剂透光率高于80%,呈现透明的溶胶状态,才是导向剂的活性期.采用XRD、IR、SEM、N2静态容量吸附法对结晶产物的晶体结构、骨架振动信息、外观形貌、比表面以及孔分布进行了表征.结果显示,该导向剂具有较好的导向活性,在老化时间为20.5 h,加入量为10%时,原位合成出了结晶度较高、无杂晶的高硅NaY分子筛.所得分子筛的硅铝比大于5.0,比表面为771 m2/g、孔径集中在0.51 nm.     

Direct Estimation of the Surface Location of Immobilized Functional Groups for Concerted Catalysis Using a Probe Molecule

The location of active sites during concerted catalysis by a metal complex and tertiary amine on a SiO₂ surface is discussed based on the interaction between the functionalized SiO₂ surface and a probe molecule, p‐formyl phenylboronic acid. The interactions of the probe molecule with the surface functionalities, diamine ligand, and tertiary amine, were analyzed by FT‐IR and solid‐state ¹³C and ¹¹B MAS NMR. For the catalyst exhibiting high 1,4‐addition activity, the diamine ligand and tertiary amine base exist in closer proximity than in the catalyst with low activity.