Hetisane - Type Diterpenoid Alkaloids

Data in the literature up to 1998 on hetisane-type diterpenoid alkaloids isolated from plants of the generaAconitum, Consolida, Delphinium, Thalictrum,andSpireaare reviewed and systematized. Data on the chemistry and physicochemical methods of studying the structures and pharmacology of hetisane alkaloids are generalized. An alphabetical list of 107 hetisane alkaloids with an indication of the plant source, structure, characteristic derivatives, physicochemical data, and references to the original literature is presented     

Diterpenoid Alkaloids from Aconitum Nagarum var. Lasiandrum

Aconitum species have been used as a traditional chinese medicine having analgesic activity. Several groups 1-3 have studied the components of the roots of Aconitum nagarum var. lasiandrum W.T. Wang. Further chemical investigation of this plant led to isolate two new diterpenoid alkaloids, nagadine 1 and 14-benzoylsachaconitine 2. This type of norditerpenoid alkaloid has not been reported before.Nagadine 1, +43.0 (c, 0.00273, CHCl3), was isolated as white amorphous powder. The HREIMS of …     

Four New Nor‐Diterpenoid Alkaloids from Aconitum brachypodum

Four new C₁₉‐nor‐diterpenoid alkaloids, named brachyaconitines A–D ( 1 – 4 ), were isolated from the roots of Aconitum brachypodum Diels. Their structures were elucidated as 3‐O‐acetyl‐20‐deethyl‐20‐formylaconitine ( 1 ), 3‐O‐acetyl‐19,20‐didehydro‐20‐deethylaconitine ( 2 ), 3‐O‐acetyl‐8‐de(acetyloxy)‐7,8,17,20‐tetradehydro‐20‐deethyl‐7,17‐secoaconitine ( 3 ), and 1‐O‐methylflavaconitine ( 4 ) by means of MS, IR, 1D‐ and 2D‐NMR analyses. The structure of compound 1 was confirmed by an X‐ray diffraction experiment.     

A new C<Subscript>19</Subscript>-diterpenoid alkaloid,habaenine C,from <Emphasis Type="Italic">Aconitum habaense</Emphasis>

A new C₁₉-diterpenoid alkaloid, habaenine C (1), together with the two known compounds vilmorrianine C and crassicauline A, were isolated from Aconitum habaense. The structure of the new compound was elucidated on the basis of spectral analysis, including 2D NMR spectroscopy. Published in Khimiya Prirodnykh Soedinenii, No. 3, pp. 265–266, May–June, 2008.     

松潘乌头和展毛多根乌头挥发油的GC／MS分析

松潘乌头(Aconitum sungpanense Hand.-Mazz.)为毛茛科乌头属植物,根有剧毒,供药用,可治疗跌打损伤、风湿性关节痛等症.主产于甘肃南部、四川北部、青海东部等地.我们曾对其进行了二萜生物碱的分离鉴定.展毛多根乌头(A.Karakolicum var.patentipilumW.T.Wang)是新疆民间常用草药之一,民间称为草乌,主治神经痛、牙痛等症.我们曾对     

DSC and NMR Study on the Inclusion Complex of Lappaconitine with β—Cyclodextrin

The inclusion complex of lappaconitine(Lap) with β-cyclodextrin (β-CD) has been studied by the differential scanning calorimetry (DSC) and ^1H-NMR,2D-NMR spectroscopy. The structure of the complex has been deduced.     

Habaenines A and B,Two New Norditerpenoid Alkaloids from Aconitum habaense

Two new norditerpenoid alkaloids, habaenine A and B ( 1 and 2 ), together with two known compounds, were isolated from Aconitum habaense. The structures of the new compounds were elucidated on the basis of spectral analyses as (1α,6α,16β)‐8‐(acetyloxy)‐20‐ethyl‐13‐hydroxy‐1,6,16‐trimethoxy‐4‐(methoxymethyl)‐19‐oxoaconitan‐14‐yl 4‐methoxybenzoate ( 1 ) and (1α,6α,16β)‐8‐(acetyloxy)‐13‐hydroxy‐1,6,16‐trimethoxy‐4‐(methoxymethyl)‐aconitan‐14‐yl 4‐methoxybenzoate ( 2 ).     

人参与附子、黄连配伍的HPLC-ESI-MS研究及抗氧化活性测定

采用高效液相色谱与电喷雾质谱联用技术(HPLC-ESI-MS)对不同比例的人参与附子或黄连的水煎液、药渣和沉淀进行研究, 共鉴定了8种人参皂苷, 发现加入附子或黄连后, 溶液中人参皂苷含量明显减少, 进一步研究证明附子或黄连中的某些成分阻止了皂苷的溶出; 同时以抗坏血酸作对照, 应用铁离子还原/抗氧化能力测定法(FRAP)测定了人参单煎液及人参与附子、黄连共煎液中正丁醇提取物和水提取物的抗氧化活性. 结果证明, 共煎液中正丁醇提取物的抗氧化活性高于人参单煎液, 同时也高于人参与附子或黄连单煎混合液的抗氧化活性, 但水提取物抗氧化活性的变化规律有所不同, 这可能与煎煮过程中物质之间的相互作用有关.     

LC‐MS analysis for the components captured by ECV304 cell from extract of Aconitum szechenyianum Gay.

A novel method of cell affinity screening (CAS), cell affinity capture coupled with LC‐MS analysis, was developed for screening the bioactive compounds related to cardiovascular diseases from the natural product libraries. One of the major characteristics lies in its function in affinity‐capturing and separating the bioactive components from the natural product libraries in vitro. Another characteristic is its use in analyzing and identifying the target compounds, by employing high‐performance liquid chromatography and mass spectrometry. CAS was used for screening the bioactive components from the alkaloid extract derived from Aconitum szechenyianum Gay. Of the five components found to be bound to the oxidative‐damaged endothelial cells, the two compounds identified, mesaconitine and aconitine, were recognized in the literature as being related to cardiovascular diseases. Copyright © 2008 John Wiley & Sons, Ltd.     

草乌中二萜类生物碱的电喷雾串联质谱研究

利用电喷雾质谱技术对传统中药草乌中的二萜类生物碱进行直接分析鉴定.通过实验数据并对比文献发现,草乌中含有单酯型、双酯型、三酯型和脂类等共4种类型生物碱,其中三酯型和脂类生物碱在草乌中为首次发现.由于这些生物碱的结构相似,在电喷雾串联质谱中碎裂方式相同,因此根据电喷雾串联质谱结果确定了这些生物碱的结构.