Structural Elucidation and Antitumor Activity of Polysaccharide AMP-1 from Atractylodis macrocephalae K.

ＩｎｔｒｏｄｕｃｔｉｏｎＡｔｒａｃｔｙｌｏｄｅｓｍａｃｒｏｃｅｐｈａｌａＫｏｉｄｚｉｓａｔｒａｄｉｔｉｏｎａｌＣｈｉｎｅｓｅｍｅｄｉｃｉｎｅ.Ｉｔｗａｓｒｅｐｏｒｔｅｄｔｈａｔｉｔｈａｓｔｈｅｓｅａｃｔｉｖｉｔｉｅｓ :“(1)ｉｎｖｉｇｏｒａｔｉｎｇｔｈｅｓｐｌｅｅｎａｎｄｂｅｎｅｆｉｔｉｎｇｖｉｔａｌｅｎｅｒｇｙ ;(2 )ｄｅ ｐｒｉｖｉｎｇｄａｍｐｎｅｓｓａｎｄｐｒｏｍｏｔｉｎｇｄｉｕｒｅｓｉｓ ;(3)ｓｔｒｅｎｇｔｈｅｎｉｎｇｓｕｐｅｒｆｉｃｉｅｓａｎｄａｎｔｉｐｅｒｓｐｉｒａｔｉｏｎ” .1Ｍｏｄｅｒ…     

多糖磷酸酯化的研究进展

简述了糖类磷酸酯的研究进展及其相应衍生物的制备方法。     

Optimum conditions for transformed Panax ginseng hairy roots in flask culture

Panax ginseng hairy roots were transformed by Agrobacterium rhizogenes KTCT 2744. They showed an active branching pattern and fast growth in hormone-free medium, and good growth at 23°C, pH 5.8, 1/2 MS medium, and 3% sucrose. Sucrose provided the highest growth among seven carbon sources tested. Six complex media were also tested. In the combined sugar study, hairy roots grew better on sucrose without glucose or fructose than with glucose or fructose. In the 1/2 MS basal medium, 30 mM in nitrogen and 0.62 mM phosphate salt concentration was the optimum. The growth ratio was maximal at an inoculum size of 0.4% (w/v). Crude saponin and polysaccharide levels were also measured.     

Polysaccharides of Saponin-Bearing Plants. XIV. Structural Study of Glucoarabinogalactan from Acanthophyllum pungens Roots

The structure of the polysaccharide glucoarabinogalactan, which is a branched polysaccharide, was established by chemical and spectral methods. Its main chain consists of -16-bound galactopyranose units. Galactopyranose atoms C-2 and C-3 act as branching points. The side chains contain galactopyranose, glucopyranose, and chains of -(13)-bound arabinopyranose units. The linear structures of three galactooligosaccharides were identified and proved by partial acid hydrolysis.     

Studies on Synthesis and Antitumor Activity of Phosphorylated Achyranthes bidentata Polysaccharide (P-AbPS)

ＩｎｔｒｏｄｕｃｔｉｏｎＩｔｉｓｗｅｌｌｋｎｏｗｎｔｈａｔｔｈｅｌｉｐｏｐｏｌｙｓａｃｃｈａｒｉｄｅｓａｒｅｃａ ｐａｂｌｅｏｆｒｅｖｅａｌｉｎｇａｖａｒｉｅｔｙｏｆｂｉｏｌｏｇｉｃａｌａｃｔｉｖｉｔｉｅｓｉｎｃｌｕｄ ｉｎｇｔｕｍｏｒ ｇｒｏｗｔｈｉｎｈｉｂｉｔｏｒｙａｃｔｉｖｉｔｙ .1Ｐｈｏｓｐｈｏｒｙｌａｔｉｏｎｏｆｐｏｌｙｓａｃｃｈａｒｉｄｅｓｈａｓａｔｔｒａｃｔｅｄｗｉｄｅｓｐｒｅａｄａｔｔｅｎｔｉｏｎｂｅｃａｕｓｅｉｔｗａｓｒｅｐｏｒｔｅｄｔｈａｔｔｈｅｙｓｈｏｗａｎｔｉｔｕｍｏｒａｃｔ…     

波谱分析在沙棘果多糖Hn结构研究中的应用

以波谱分析为主要手段研究沙棘果多糖Hn的结构，GC分析表明Hn由Ara，Gal，Man和Glc组成，单糖物质的量比与利用GC分析Hn甲基化产物的结果一致．^13C—NMR分析表明Hn中存在α，β型糖苷键．利用GC—MS分析进一步确定Hn主链结构由β（1→4）Glc，β（1→6）Man和β（1→4）Gal构成，在3-O处有分支，支链由α（1→4）Glc，（1→6）Gal或末端Ara，Glc构成．     

鲍鱼多糖Hal-A的甲醇解研究

从鲍鱼中分离出一种鲍鱼多糖Hal-A，并用改进甲醇解方法研究了Hal-A的组成 ，该多糖甲醇解产物经三甲硅醚衍生后，进行GC/MS，结果表明鲍鱼多糖Hal-A主要 由葡萄糖、半乳糖、甘露糖和少量木糖、岩灌糖和半乳糖醛酸组成。     

碱提猴头发酵菌丝体水溶性多糖HPP的结构确定

从猴头发酵菌丝体水提后残渣中用碱提出了水溶性粗多糖,对其分级纯化得到纯样HPP,SepharoseCL-6B柱层析测得其相对分子质量约为6 53×104,G C 分析表明,其单糖组成为Glc;经部分酸水解、高碘酸氧化、Smith降解和甲基化分析确定此多糖为多分枝结构,其中(1→6)Glc构成主链的核心结构;分支点为O-3;支链部分由(1→3)Glc构成,并连接一端基Glc.     

玉米芯酸提水溶性多糖CCCP的分离纯化和结构研究

玉米芯用PH=3的HCl煮提得到酸提水溶性粗多糖．该粗多糖组成为Glc，Xyl，Gal，经乙醇分级和Sepharose CL-6B柱层析纯化，得到多糖CCCP．经Sephadex A-25柱层析、比旋光度测定、醋酸纤维薄膜电泳等方法鉴定CCCP为均一多糖．经唾液淀粉酶解、纤维素酶解、部分酸水解、高碘酸氧化、Smith降解、甲基化分析及IR，GC和GC／MS等方法分析表明：CCCP为少分枝结构；主链由吡喃型(1→3)Xyl构成，在O(4)处有分枝；支链主要由(1→4)Glc构成，在O(6)处有分枝，支链还存在1→3，1→6键型连接的Glc，Gal；末端基为Xyl，Glc，Gal．     

Effect of the degree of deacetylation and the substitution of carboxymethyl chitosan on its aggregation behavior

The aggregation behavior of carboxymethyl chitosan (CM‐chitosan) with various degrees of deacetylation (DD) and substitution (DS) was characterized with viscometry, gel permeation chromatography (GPC), and GPC coupled with laser light scattering (GPC‐LLS). The results indicate that CM‐chitosan has a strong tendency to form aggregates in aqueous solution and the aggregation behavior depends on DD and DS values. The apparent aggregation number (N_ap), the gyration radius (R_g), and the weight fraction of the aggregates (F_a) reached maximum at a DD value of 50%, then decreased, with the DD value deviating from 50%. A higher DS value helped to form aggregates; when the DS value increased from 0.65 to above 1.0, N_ap and R_g increased sharply. The dependence of the refractive index increment (dn/dc) on the DD and DS values was related to variation of the charge density and the hydrophobic interaction along the molecular chains. The conformations of CM‐chitosan aggregates were studied by the LLS method. The aggregates showed a spherical shape, and the chain stiffness increased with introduction of the acetyl groups. The DS value had no clear influence on the chain conformation that was observed. © 2004 Wiley Periodicals, Inc. J Polym Sci Part B: Polym Phys 43: 296–305, 2005