分离检测生物活性物质的荧光标记试剂与分子探针及其应用

作者结合自己的研究工作主要评述了用于分离检测氨基化合物（氨基酸、肽、蛋白质和生物胺等）、巯基化合物（谷胱甘肽、半胱氨酸和高半胱氨酸等）及NO等生物活性物质的荧光标记试剂和荧光分子探针的近期进展和应用。除了传统的的OPA、NDA、DNS、FMOC、FITC、NBD-F、AQC、Cy5等在HPLC和CE分离荧光检测应用新进展,还介绍了许多新的荧光分子探针和标记试剂的性能和应用。它们是N－羟基琥珀酰亚胺活性酯荧光标记试剂,6-氧-（N-琥珀酰亚胺乙酸酯）-9-（2'-甲氧羰基）荧光素（SAMF）,1,3,5,7-四甲基-8-苯基-（4'-O-（N-琥珀酰亚胺乙酸酯））-二氟化硼-二吡咯甲烷（TMPAB-Osu,）等和3-碘乙酰胺苯嵌蒽酮荧光探针及MCY5、DSTCY、DCDSTCY和DCTCY等花菁类近红外荧光探针等。     

Paternò-Büchi反应的区域选择性

Paternò-Büchi(P-B)反应,即羰基-烯的[2+2]光环化加成反应,利用其特殊的区域及立体选择性可以合成一些结构精巧的取代氧杂环丁烷。随着P-B反应在有机合成中越来越广泛的应用,人们对P-B反应的区域选择性的研究也越来越深入。在P-B反应发现之初,人们一直用“最稳定的双自由基规则”解释其区域选择性,这一规则对有些体系却不适用,近年来有人开始运用“自旋化学”理论来解释区域选择性。本论文结合作者的研究工作,评述了近年来P-B反应的区域选择性的研究进展,重点阐述了反应温度、反应物的取代基对P-B反应区域选择性的影响及其规律。     

Ion trap mass spectrometry on a comet nucleus: the Ptolemy instrument and the Rosetta space mission

In May 2014, the Rosetta spacecraft is scheduled to rendezvous with the comet Churyumov-Gerasimenko ('67P'). One of the instruments on board the 'Lander' which will descend on to the surface of the comet is a miniaturised GC/MS system that incorporates an ion trap mass spectrometer, specially developed for isotope ratio analysis. This article describes the development and optimisation of the ion trap for this unique application, and presents a summary of the range of pre-programmed experiments that will contribute to the characterisation of the solid and volatile cometary materials.     

Synthesis of novel 1,4,7,10-tetraazacyclodecane-1,4,7,10-tetraacetic acid (DOTA) derivatives for chemoselective attachment to unprotected polyfunctionalized compounds

A convenient synthesis of novel bifunctional poly(amino carboxylate) chelating agents allowing chemoselective attachment to highly functionalized biomolecules is described. Based on the well known chelator 1,4,7,10-tetraazacyclodecane-1,4,7,10-tetraacetic acid (DOTA), we synthesized novel bifunctional chelating agents bearing additional functional groups by alkylating 1,4,7,10-tetraazacyclododecane (cyclen) with one equivalent of para-functionalized alkyl 2-bromophenyl-acetate and three equivalents of tert-butyl 2-bromoacetate. The resulting compounds, which contain an additional carbonyl or alkyne functionality, allow site specific labeling of appropriately functionalized unprotected biomolecules in a rapid manner via click reactions. This was demonstrated by the attachment of our new DOTA derivatives to the somatostatin analogue Tyr3-octreotate by chemoselective oxime ligation and CuI-catalyzed azide-alkyne cycloaddition. Initial biodistribution studies in mice with the radiometalated compound demonstrated the applicability of the described DOTA conjugation.     

Improved protein-adsorption resistance of polyethersulfone membranes via surface segregation of ultrahigh molecular weight poly(styrene-alt-maleic anhydride)

A styrene-maleic anhydride (SMA) alternating copolymer with ultrahigh molecular weight (M_w > 10⁶) synthesized in super critical carbon dioxide (SC CO₂) medium was used as hydrophilic polymeric additive in the preparation of polyethersulfone (PES) membranes. The PES/SMA blend membranes were prepared by immersion precipitation process. X-ray photoelectronic spectroscopy (XPS) measurements confirmed that the hydrolyzed SMA preferentially segregated to membrane–coagulant interface during membrane formation. For the PES/SMA blend membranes, no big change was observed in the cross-sectional structure and the mechanical properties were well maintained after SMA addition except that a thicker top layer was formed. The surface morphology analysis by atomic force microscopy (AFM) showed that the membrane surface roughness increased with the added SMA amount. The results of water contact angle, water absorbance measurements and static protein adsorption experiments revealed that the surface enrichment of SMA endowed PES/SMA blend membranes with significantly improved surface hydrophilicity and protein-adsorption resistance.     

Minimal polynomials and radii of elements in finite-dimensional power-associative algebras

In the first section of this paper we revisit the definition and some of the properties of the minimal polynomial of an element of a finite-dimensional power-associative algebra over an arbitrary field . Our main observation is that , the minimal polynomial of , may depend not only on , but also on the underlying algebra. More precisely, if is a subalgebra of , and if is the minimal polynomial of in , then may differ from , in which case we have .

In the second section we restrict attention to the case where is either the real or the complex numbers, and define , the radius of an element in , to be the largest root in absolute value of the minimal polynomial of . We show that possesses some of the familiar properties of the classical spectral radius. In particular, we prove that is a continuous function on .

In the third and last section, we deal with stability of subnorms acting on subsets of finite-dimensional power-associative algebras. Following a brief survey, we enhance our understanding of the subject with the help of our findings of the previous section. Our main new result states that if , a subset of an algebra , satisfies certain assumptions, and is a continuous subnorm on , then is stable on if and only if majorizes the radius defined above.

     

On the Convergence of Products γ^-sh1hn in the Adams Spectral Sequence

Abstract Let A be the mod p Steenrod algebra and S the sphere spectrum localized at p, where p is an odd prime. In 2001 Lin detected a new family in the stable homotopy of spheres which is represented by （b0hn-h1bn-1）∈ ExtA^3,（p^n＋p）q（Zp,Zp） in the Adams spectral sequence. At the same time, he proved that i.（hlhn） ∈ExtA^2,（p^n＋P）q（H^＊M, Zp） is a permanent cycle in the Adams spectral sequence and converges to a nontrivial element ξn∈π（p^n＋p）q-2M. In this paper, with Lin＇s results, we make use of the Adams spectral sequence and the May spectral sequence to detect a new nontrivial family of homotopy elements jj′j^-γsi^-i′ξn in the stable homotopy groups of spheres. The new one is of degree p^nq ＋ sp^2q ＋ spq ＋ （s - 2）q ＋ s - 6 and is represented up to a nonzero scalar by hlhnγ-s in the E2^s＋2,＊-term of the Adams spectral sequence, where p ≥ 7, q = 2（p - 1）, n ≥ 4 and 3 ≤ s 〈 p.     

稳态问题混合边界积分方程的高精度求积法与分裂外推

提出了求积法解稳态问题的混合边界积分方程,它拥有高精度,低复杂度.通过并行地解粗网格上的离散方程,根据误差的多参数渐近展开,应用分裂外推算法得到高精度的近似解,同时获得后验误差估计.     

TOPOLOGICAL STRUCTURE OF THE SINGULAR POINTS OF THE THIRD ORDER PHASE LOCKED LOOP EQUATIONS WITH THE CHARACTER OF DETECTED PHASE BEING g(φ)=(1+k)sinφ/(1+kcosφ)

In this paper, we study the topological structure of the singular points of the third order phase locked loop equations with the character of detected phase being g(φ)=(1+k)sinφ/1+kcosφ.