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目前中药制剂生产过程中缺乏全过程参数检测和质量控制技术手段,不同生产批次药品化学成分差异较大、质量不够稳定、临床使用疗效和安全性不理想,因此,建立其完善的质量评价体系及其准确快速的质量评价方法,成为中药质量控制的重中之重.通过对刺五加注射液近红外(near infrared,NIR)含量预测模型的谱段选择规律和消除溶剂干扰方法的探讨,发现采用表征混合物结构差异的结构相关谱段结合含量相关谱段作为NIR组分预测模型谱段,用基于水为参比光谱的样本光谱建立含量预测模型,并利用水作为参比光谱识别和提取待分析组分的光谱信息,可以得到较理想的NIR含量预测结果.通过对刺五加注射液中绿原酸、紫丁香苷和刺五加苷E组分的近红外光谱结构相关谱段和含量相关谱段的归属,分别建立了绿原酸、紫丁香苷和刺五加苷E组分的含量预测模型,可用于快速分析刺五加注射液中不同组分的含量. 相似文献
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Simultaneous determination and pharmacokinetics of four triterpenoids by ultra high performance liquid chromatography with tandem mass spectrometry after the oral administration of Acanthopanax sessiliflorus (Rupr. et Maxim) Seem extract
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Yang Song Zhibin Wang Xuesong Feng Xiaolan Deng Juan Zhu 《Journal of separation science》2016,39(12):2229-2237
A specific, simple, and sensitive ultra high performance liquid chromatography with tandem mass spectrometry method utilizing the Triple Quad system has been developed and validated for the simultaneous determination and pharmacokinetic study of four triterpenoid components of Acanthopanax sessiliflorus in rat plasma. The components are 22‐α‐hydroxychiisanogenin, chiisanogenin, (1R,11α)1,4‐epoxy‐11‐hrdroxy‐3,4‐secolupane‐20(30)‐ene‐3,28‐dioic acid, and 22‐α‐hydroxychiisanoside. Sample preparation involved a liquid–liquid extraction of the analytes with ethyl acetate. Chromatographic separation was accomplished using an Agilent SB‐C18 column (1.8 μm, 2.1 mm × 50 mm) with 2.0 min isocratic elution. The compounds were detected with a triple quadrupole tandem mass spectrometer in multiple reaction monitoring mode and an ESI source in negative mode. The method was linear for all analytes over the investigated range, with all determined correlation coefficients exceeding 0.9906. The limit of quantification of each analyte was lower than 1 ng/mL. The intraday and interday precisions were less than 14.9%, and the accuracy ranged from –10.2 to 11.8%. The mean recoveries of the analytes were higher than 80.0%, and the matrix effects were between 100.4 and 107.1%. These results may contribute to determining the mechanism of action and guiding the clinical application of Acanthopanax sessiliflorus. 相似文献
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Qian Wang Yixiang Wang Ran Liu Xu Yan Yujiao Li Hui Fu Kaishun Bi Qing Li 《Biomedical chromatography : BMC》2013,27(2):208-215
A simple and sensitive method for the simultaneous determination of plasma concentrations of five polyamines in normal and Hepatoma‐22 mice, and mice treated with Mylabris and Acanthopanax senticosus was developed by HPLC‐ESI‐MS. Male Kunming mice were divided into nine groups, a control group (inoculation without treatment), a positive group (Cyclophosphamide), treatment groups [Mylabris (4, 8, 16 mg/kg), Acanthopanax senticosus (6, 12, 24 g/kg)] and a normal group (without inoculation). Twenty‐four hours after the last administration, plasma samples were collected. The derived polyamines were separated on a C18 column by a gradient elution using methanol–water with excellent linearity within the range from 2.5 to 1000 ng/mL. Polyamines were confirmed as useful biochemical markers of hepatoma. The differences in anti‐cancer therapeutic efficacy between Mylabris and Acanthopanax senticosus might contribute to the variability of polyamine levels in vivo. This HPLC‐ESI‐MS method was successfully applied to investigate the relationship between polyamines and cancer in mice and might be a useful method to test the activity of potential anti‐tumor drugs. Copyright © 2012 John Wiley & Sons, Ltd. 相似文献
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We elucidated the structure and metabolite profile of eleutheroside B, a component derived from the extract of Acanthopanax senticosus Harms, after oral administration of the extract in rats. Samples of rat plasma were collected and analyzed by selective high‐resolution liquid chromatography/quadrupole time‐of‐flight mass spectrometry (UPLC/Q‐TOF MS) automated data analysis method. A total of 11 metabolites were detected: four were identified, and three of those four are reported for the first time here. The three new plasma metabolites were identified on the basis of mass fragmentation patterns and literature reports. The major in vivo metabolic processes associated with eleutheroside B in A. senticosus include demethylation, acetylation, oxidation and glucuronidation after deglycosylation. A fairly comprehensive metabolic pathway was proposed for eleutheroside B. Our results provide a meaningful basis for drug discovery, design and clinical applications related to A. senticosus in traditional Chinese medicine. Copyright © 2012 John Wiley & Sons, Ltd. 相似文献
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Wanying Yu Hua Zhang Weidong Huang Jiping Chen Xinmiao Liang 《Frontiers of Chemistry in China》2006,1(2):193-198
Gas chromatography/quadrupole mass spectrometry (GC/qMS), gas chromatography/Fourier transform infrared spectrometry (GC/FTIR)
and gas chromatography/orthogonal acceleration time-of-flight mass spectrometry (GC/oaTOFMS) were applied to analyze the volatile
oil from the stem of Acanthopanax Senticosus (Rupr. et Maxim.) Harms. Based on the mass spectra search function of GC/qMS with the aid of the discriminability of the
geometrical isomer by GC/FTIR and the ability to determine the accurate mass charge ratio (m/z) by GC/oaTOFMS, 68 GC eluants were identified successfully. Compared with the results obtained by GC/qMS only, the analytical
results obtained by these hyphenated methods of gas chromatography are more credible.
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Translated from Chinese Journal of Chromatography, 2005, 23(2)(in Chinese) 相似文献
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The redox reaction is a normal process of biological metabolism in the body that leads to the production of free radicals. Under conditions such as pathogenic infection, stress, and drug exposure, free radicals can exceed normal levels, causing protein denaturation, DNA damage, and the oxidation of the cell membrane, which, in turn, causes inflammation. Acanthopanax senticosus (A. senticosus) flavonoids are the main bioactive ingredients with antioxidant function. H2O2-treated RAW 264.7 cells and DSS-induced colitis in mice were used to evaluate the antioxidant properties of A. senticosus flavonoids. The results show that A. senticosus flavonoids can significantly downregulate the levels of ROS and MDA in H2O2-treated RAW 264.7 cells and increase the levels of CAT, SOD, and GPx. A. senticosus flavonoids can also increase the body weights of DSS-induced colitis mice, increase the DAI index, and ameliorate the shortening of the colon. ELISA experiments confirmed that A. senticosus flavonoids could reduce the level of MDA in the mouse serum and increase the levels of SOD, CAT, and GPx. Histopathology showed that the tissue pathological changes in the A. senticosus flavonoid group were significantly lower than those in the DSS group. The Western blot experiments showed that the antioxidant capacity of A. senticosus flavonoids was accomplished through the Nrf2 pathway. In conclusion, A. senticosus flavonoids can relieve oxidative stress in vivo and in vitro and protect cells or tissues from oxidative damage. 相似文献