Free and Sulfated Sterols of Two Far-East Leptasterias Starfish

NMR spectroscopy, capillary GLC, and GLC-MS are used to study the composition of free and sulfated sterols from the far-east starfishLeptasterias alaskensis asiatica(Fischer) andL. Fisheri(Djakonov). The total free sterols of both species are shown to have similar qualitative and quantitative compositions and contain mainly⁷ -sterols. Sterol sulfate fractions contain cholesterol sulfate as the main component but differ in the ratios of⁵ :⁰ :⁷ -sterol derivatives. Possible reasons for these differences are discussed. A new steroid, 3-hydroxycholest-5-en-7-one sulfate, was isolated.     

Novel Synthesis of 2β,3β,20β-Triacetoxy-5 -pregnan-6-one

Pregnenolone (2) is used in a novel synthesis of 2,3,20-triacetoxy-6-ketosteroid (6), a key intermediate in the preparation of 20-hydroxyecdysone (1)     

Chemical Composition of Certain Sedum Species of Kazakhstan

The chemical composition of sevenSedumspecies growing in Kazakhstan was studied by chromatography. The contents of seven groups of natural compounds were determined. Comparison with markers and literature data identified 24 compounds including 9 that have not been described previously inSedumspecies     

Partial Molar Volumes of Transfer of Some Amino Acids from Water to Aqueous Glycerol Solutions at 25°C

Apparent molar volumes of glycine, DL--alanine, L-valine, L-leucine, and L-phenylalanine in 0.5, 1.0, 2.0, 3.5, and 5.0 m _B (mol-kg^–1) aqueous solutions of glycerol have been obtained from solution densities at 25°C using precise vibrating-tube digital densimeter. The estimated partial molar volumes at infinite dilution V ^o ₂ have been used to obtain the corresponding transfer volumes _tr V ₂ ^o from water to different glycerol–water mixtures. The transfer volumes are positive for glycine and DL--alanine, and both positive and negative for the other amino acids over the concentration range studied. Interaction coefficients have been obtained from McMillan–Mayer approach and the data have been interpreted in terms of solute–cosolute interactions.     

On the commutator of the Marcinkiewicz integral

boundedness is considered for the commutator of higher-dimensional Marcinkiewicz integral. Some conditions implying the and the boundedness for the commutator of the Marcinkiewicz integral are obtained.     

Pseudo-Anosov Flows and Incompressible Tori

We study incompressible tori in 3-manifolds supporting pseudo-Anosov flows and more generally ZZ subgroups of the fundamental group of such a manifold. If no element in this subgroup can be represented by a closed orbit of the pseudo-Anosov flow, we prove that the flow is topologically conjugate to a suspension of an Anosov diffeomorphism of the torus. In particular it is non singular and is an Anosov flow. It follows that either a pseudo-Anosov flow is topologically conjugate to a suspension Anosov flow, or any immersed incompressible torus can be realized as a free homotopy from a closed orbit of the flow to itself. The key tool is an analysis of group actions on non-Hausdorff trees, also known as R-order trees – we produce an invariant axis in the free action case. An application of these results is the following: suppose the manifold has an R-covered foliation transverse to a pseudo-Anosov flow. If the flow is not an R-covered Anosov flow, then it follows that the manifold is atoroidal.     

Function Spaces of Posets with Projections

This paper investigates function spaces of structures consisting of a partially ordered set together with some directed family of projections.More precisely, given a fixed directed index set (I,), we consider triples (D,,(p _i ) _iI ) with (D,) a poset and (p _i ) _iI a monotone net of projections of D. We call them (I,)-pop's (posets with projections). Our main purpose is to study structure preserving maps between (I,)-pop's. Such homomorphisms respect both order and projections.Any (I,)-pop is known to induce a uniformity and thus a topology. The set of all homomorphisms between two (I,)-pop's turns out to form an (I,)-pop itself. We show that its uniformity is the uniformity of uniform convergence. This enables us to prove that properties such as completeness and compactness transfer to function pop's.Concerning categorical properties of (I,)-pop's, we will see that we are in a lucky situation from a computer scientist's point of view: we obtain Cartesian closed categories. Moreover, by a D -construction we get (I,)-pop's that are isomorphic to their own exponent. This yields new models for the untyped -calculus.     

Crystal structures of 4,7-phenanthrolino-5,6:5′,6′-pyrazine tetrahydrate and 1,4,5,8,9,12-hexaazatriphenylene dihydrate

The crystal structures of 4,7-phenanthrolino-5,6:5,6-pyrazine tetrahydrate (I) and 1,4,5,8,9,12-hexaazatriphenylene dihydrate (II) have been determined from low temperature (173 K) x-ray single crystal diffraction data. I crystallizes in the triclinic system, space group P with a = 11.2687(6), b = 12.4766(6), c = 12.7068(7) Å, = 113.4740(10), = 91.605(2), = 114.587(2)°, V = 1449.33(13) ų, and Z = 4. II crystallizes in the orthorhombic system, space group Pca2₁ with a = 18.187(2), b = 9.2576(11), c = 6.9672(8) Å, V = 1173.0(2) ų, and Z = 4. In both structures the planar heterocyclic molecules stack with interplanar distances down to 3.3 Å, which is consistent with self--complexation. The crystal water molecules provide links between the columns of stacked molecules through hydrogen bonds. In each compound the N-C bond lengths fall into two distinct groups (mean values 1.330(3) vs 1.361(1) Å in I and 1.320(3) vs 1.357(5) Å in II), as do the C-C bonds of the central phenyl rings (1.411(4) vs 1.467(1) Å in I and 1.405(5) vs 1.458(4) Å in II).     

重离子辐射提高腺病毒介导小鼠黑色素瘤细胞转染率的研究

探讨12C6+ 离子束辐射对用带有绿色荧光蛋白基因的缺陷性腺病毒（AdCMV GFP）转染小鼠黑色素瘤细胞(B16细胞系)的影响。 采用不同剂量的12C6+ 重离子束辐射经AdCMV GFP 转染的B16细胞， 利用流式细胞仪检测腺病毒的转染率。 结果表明， 12C6＋重离子束辐射能提高腺病毒对B16细胞的转染率， 且具有量效关系。 此外， 先转染后辐射法比起先辐射后转染法能更显著地提高转染率。The effect of 12C6+ beam irradiation on AdCMV GFP (a replication deficient recombinant adenoviral vector containing CMV promoter and green fluorescent protein) gene transfection efficiency for murine melanoma cell B16 has been investigated. B16 cells infected with AdCMV GFP were irradiated by different doses of 12C6+ beam. The transfection efficiency was assessed by flow cytometry (FCM). Results show that 12C6+ beam irradiation can improve tansfection efficiency of AdCMV GFP on murine melanoma cell B16 in a dose dependent manner. In addition， the tansfection efficiency in pre tranfection plus irradiation group is higher than that in pre irradiation plus tranfection group at the same dose irradiation dose.     

多晶LaB6阴极的脉冲热发射特性

 采用多晶LaB₆材料制成平板二极管阴极，阳极采用钼材料，阴极采用热传导与热辐射加热，加热体为石墨。实验研究了不同阴极温度、不同真空度下的脉冲发射特性，并对热发射稳定性进行了分析。结果表明： 在动态真空系统中， 阴极发射面积为0.012 1 cm²， 工作真空度为2×10^-4 Pa， 阴极温度分别为1 600， 1 650和1 700 ℃，在脉冲宽度为40 ms、重复频率为107 Hz的条件下，最大脉冲发射电流密度分别为34.0，44.0和53.8 A/cm²; 2×10^-4，5×10^-4和2×10^-3 Pa压强下的发射能力没有明显的差异;脉冲宽度的变化不影响发射电流密度的变化。