排序方式: 共有40条查询结果,搜索用时 0 毫秒
11.
N. Nassif A. Coiffier T. Coradin C. Roux J. Livage O. Bouvet 《Journal of Sol-Gel Science and Technology》2003,26(1-3):1141-1144
Whole E. coli bacteria have been trapped within silica gels obtained via the acidification of sodium silicate and silica nanoparticles solutions. Their -galactosidase enzymatic activity increases with time, suggesting that their membrane is partially lysed during the encapsulation process. Such a lysis can be greatly reduced when encapsulation is performed in the presence of gelatin. The biocatalytic activity of trapped bacteria remains almost constant for more than a week. Moreover bacteria trapped in such gels remain able to incorporate glucose, showing that their viability has been preserved. 相似文献
12.
13.
This study investigated the properties of immobilized β-galactosidase on polymeric beads having Schiff base. Polystyrene microspheres attached L-Alanine (FMPS-Ala) was synthesized from (4-formyl-3-methoxyphenoxymethyl)polystyrene (FMPS) and L-alanine by condensation. A coordinasyon polymer involving Ni2+(FMPS-Ala-Ni) was produced with the template method and characterized. β-galactosidase was immobilized onto the (FMPS), (FMPS-Ala) and (FMPS-Ala-Ni) complexes via covalent bonds. The Km/Vmax values were calculated as 0.343 mM/0.0259 mM min?1for free β-galactosidase and 0.104 mM/0.0126 mM min?1, 0.0617 mM/0.0417 mM min?1and 0.210 mM/0.0287 mM min?1for β-galactosidase immobilized to the (FMPS), (FMPS-Ala) and (FMPS-Ala-Ni) supports, respectively. The storage stability of (FMPS-Ala-Ni) was determined to be higher than that of the (FMPS) and (FMPS-Ala) polymers. 相似文献
14.
《Electroanalysis》2018,30(5):852-858
In this study, a novel signal‐amplified strategy for sensitive electrochemical sandwiched immunoassay of carcinoembryonic antigen (CEA) was constructed based on aminofunctionalized graphene oxide (GO‐NH2) supported AgNPs used as catalytic labels of secondary anti‐CEA and β‐galactosidase (β‐Gal), Meanwhile, sulfhydrylation single‐wall carbon nanotubes (SWCNTs‐SH) as substrate materials embellished gold electrode through Au‐SH and connected with gold nanoparticles to form anti‐CEA/AuNPs/SWCNTs‐SH/Au sensing platform through layer‐by‐layer. In the presence of analyte CEA, a sandwich‐type immunoassay format was employed for determination of CEA by using the labeled β‐Gal toward the reduction of p‐aminophenyl galactopyranoside (PAPG) and the redox reaction of AgNPs. Under optimal conditions, the increase in the current was proportional to the concentration of CEA from 0.1 pg/mL to 200 ng/mL. The detection limit (LOD) was 0.036 pg/mL CEA at 3σ. The electrochemical immunoassay displayed an acceptable precision, selectivity, stability. Clinical serum specimens were assayed with the method, and the results were in acceptable agreement with those obtained from the referenced electrochemiluminescent method. 相似文献
15.
α-Galacto-oligosaccharides (α-GOSs) have great functions as prebiotics and therapeutics. This work established the method of batch synthesis of α-GOSs by immobilized α-galactosidase for the first time, laying a foundation for industrial applications in the future. The α-galactosidase from Aspergillus niger L63 was immobilized as cross-linked enzyme aggregates (CLEAs) nano-biocatalyst through enzyme precipitating and cross-linking steps without using carriers. Among the tested agents, the ammonium sulfate showed high precipitation efficacy and induced regular structures of α-galactosidase CLEAs (Aga-CLEAs) that had been analyzed by scanning electron microscopy and Fourier-transform infrared spectroscopy. Through optimization by response surface methodology, the ammonium sulfate-induced Aga-CLEAs achieved a high activity recovery of around 90% at 0.55 U/mL of enzymes and 36.43 mM glutaraldehyde with cross-linking for 1.71 h. Aga-CLEAs showed increased thermal stability and organic solvent tolerance. The storage ability was also improved since it maintained 74.5% activity after storing at 4 °C for three months, significantly higher than that of the free enzyme (21.6%). Moreover, Aga-CLEAs exhibited excellent reusability in the α-GOSs synthesis from galactose, retaining above 66% of enzyme activity after 10 batch reactions, with product yields all above 30%. 相似文献
16.
Genetically controlled MRI contrast mechanisms and their prospects in systems neuroscience research 总被引:1,自引:0,他引:1
Application of MRI contrast agents to neural systems research is complicated by the need to deliver agents past the blood-brain barrier or into cells, and the difficulty of targeting agents to specific brain structures or cell types. In the future, these barriers may be wholly or partially overcome using genetic methods for producing and directing MRI contrast. Here we review MRI contrast mechanisms that have used gene expression to manipulate MRI signal in cultured cells or in living animals. We discuss both fully genetic systems involving endogenous biosynthesis of contrast agents, and semi-genetic systems in which expressed proteins influence the localization or activity of exogenous contrast agents. We close by considering which contrast-generating mechanisms might be most suitable for applications in neuroscience, and we ask how genetic control machinery could be productively combined with existing molecular agents to enable next-generation neuroimaging experiments. 相似文献
17.
从鹰嘴豆中分离得到了三种β-半乳糖苷酶(酶Ⅰ、酶Ⅱ和酶Ⅲ)。将酶Ⅰ和酶Ⅱ进一步纯化,其比活力分别提高了19倍和48倍.酶活力回收率分别为16%和18%,测得它们的表观分子里分别为2.4×104和5.8×104;最适pH分别5.9和5.0,最适温度分别为55℃和45℃.酶Ⅰ水解ONPG和PNPG的KM分别为33×10-2mol·dm-3和60×10-3mol·dm-3;酶Ⅱ水解ONPG和PNPG的KM分别为30×10-3mol·dm-3和60×10-4mol·dm-3.乳糖和半乳糖为该酶的竞争性可逆抑制剂,棉子糖为非竞争性可逆抑制剂.该酶受Hg2+和PCMB强烈抑制和NEM明显抑制,而Mg2+、Zn2+和Ca2+具有激活作用,推知巯基(-SH)是酶活性中心必须基团. 相似文献
18.
P. Linko 《Applied biochemistry and biotechnology》1982,7(1-2):5-9
Recent work on immobilized biocatalysts at Helsinki University of Technology, Finland, is described, with starch processing,
Β-galactosidase, glucose isomerase, invertase, and the immobilization of live cells as special examples. 相似文献
19.
Functional screening studies revealed that Aspergillus carbonarius ATCC6276 produced extracellular β-galactosidase activity potentially suited for use as a lactase digestive supplement in
the treatment of lactose intolerance. The crude preparation contained two β-galactosidase activities, β-gal 1 and β-gal 2,
which were separated by ion-exchange chromatography. Both enzymes were purified to homogeneity by a combination of gel filtration,
ion-exchange, chromatofocusing and hydrophobic interaction chromatographies. β-gal 1 and β-gal 2 displayed differences in
molecular mass (110 kDa versus 120 kDa as judged by SDS PAGE) and in a range of additional physicochemical properties. Km
values of 83 and 309 mM, respectively, were recorded using lactose as substrate while temperature optima of 55°C versus 65°C
were obtained. Unlike current commercialized supplemental lactases, both of the purified enzymes displayed significant stability
when exposed to simulated gastric conditions, with β-gal 1in particular retaining 70% residual activity after exposure to
pH 2.0 in the presence of pepsin for 2 h. Overall the results indicate that the β-galactosidases of Aspergillus carbonarius ATCC6276, either individually or in combination, may be suitable for use as a digestive supplement for the alleviation of
lactose intolerance. 相似文献
20.
Jung-Young Park Gu-Hwan Kim Sung-Su Kim Jung Min Ko Jin-Joo Lee Han-Wook Yoo 《Experimental & molecular medicine》2009,41(1):1-7
Fabry disease is an X-linked inborn error of glycosphingolipid catabolism that results from mutations in the gene encoding the α-galactosidase A (GLA) enzyme. We have identified 15 distinct mutations in the GLA gene in 13 unrelated patients with classic Fabry disease and 2 unrelated patients with atypical Fabry disease. Two of the identified mutations were novel (i.e., the D231G missense mutation and the L268delfsX1 deletion mutation). This study evaluated the effects of the chemical chaperones 1-deoxygalactonojirimycin (DGJ) on the function of GLA in vitro, in cells containing missense mutations in the GLA gene. Nine missense and a nonsense mutations, including one novel mutation were cloned into mammalian expression vectors. After transient expression in COS-7 cells, GLA enzyme activity and protein expression were analyzed using fluorescence spectrophotometry and Western blot analysis, respectively. DGJ enhanced GLA enzyme activity in the M42V, I91T, R112C and F113L mutants. Interestingly, the I91T and F113L mutations are associated with the atypical form of Fabry disease. However, DGJ treatment did not have any significant effect on the GLA enzyme activity and protein expression of other mutants, including C142W, D231G, D266N, and S297F. Of note, GLA enzyme activity was not detected in the novel mutant (i.e., D231G), although protein expression was similar to the wild type. In the absence of DGJ, the E66Q mutant had wild-type levels of GLA protein expression and approximately 40% GLA activity, indicating that E66Q is either a mild mutation or a functional single nucleotide polymorphism (SNP). Thus, the results of this study suggest that the chemical chaperone DGJ enhances GLA enzyme activity and protein expression in milder mutations associated with the atypical form of Fabry disease. 相似文献