型胶原在聚-L-乳酸(PLLA)表面的化学接枝和涂层及其对软骨细胞生长的促进作用

采用接枝 -涂层技术在聚 -L-乳酸 ( PLLA)膜表面接枝聚甲基丙烯酸 ( PMAA)得到稳定的 PLLA-g-PMAA胶原涂层 .利用水溶性碳化二亚胺作为缩合剂 ,使 PLLA-g-PMAA表面的羧基和胶原分子中的氨基发生缩合反应 ,从而将 型胶原接枝在 PLLA表面 ;同时保留材料表面物理涂层的胶原溶液 ,获得了稳定的胶原涂层 . XPS谱图证实了接枝反应的发生 .分别用比色法和茚三酮法测定了 PLLA-g-PMAA表面的羧基密度和 PLLA膜表面胶原的接枝量和涂层量 .细胞培养结果表明 ,改性后的 PLLA膜表面软骨细胞的铺展性能和增长速度明显改善 .     

3-(三聚氧乙烯)取代聚噻吩的合成与聚集增强发光

通过2-溴-5-碘-3-(三聚氧乙烯)取代噻吩大分子单体的合成、镍催化缩聚等,得到Mn为6000的3-(三聚氧乙烯)取代聚噻吩(P3OET).在非选择性良溶剂四氢呋喃中,P3OET溶液发射橙色荧光.在选择性极性溶剂诱导作用下,P3OET在水中自组装形成稳定的球形自组装体,荧光峰相对红移而发射橙红色光,伴随荧光强度减弱;而在甲醇中,P3OET形成丝状自组装体,荧光峰相对蓝移而发射亮黄色光,但是荧光强度增强,表现出聚集增强发光现象.类似于含有低聚氧乙烯短支链的聚烯烃,P3OET在水溶液中还具有温度响应特性.当水溶液温度升高,荧光峰逐渐蓝移,并且荧光强度增强.P3OET自组装体可进入小鼠黑色素瘤B16F10细胞,并且发光而用于成像.利用控温热台适当加热细胞,或者使用解偶联剂、抗癌药物等化学刺激使细胞活动产生热量时,发光性质随细胞温度升高而发生蓝移、强度增强等规律性变化.     

全胸腔镜手术与胸腔镜辅助小切口手术对Ⅰ期非小细胞肺癌的临床疗效比较

目的比较全胸腔镜手术与胸腔镜辅助小切口手术对Ⅰ期非小细胞肺癌的临床疗效。方法选择86例Ⅰ期非小细胞肺癌患者,按随机数字表法分为使用全胸腔镜手术治疗组（全胸腔镜组46例,采用3孔法）及使用胸腔镜辅助小切口手术治疗组（胸腔镜辅助组40例,采用2孔法）。比较两组患者一般手术情况、术后生存质量及无瘤生存情况。结果两组患者的手术时间、术中出血量、术后带胸管时间及住院时间比较差异均无统计学意义（均P＞0.05）,全胸腔镜组患者术后胸腔液引流量[（217.8±50.5）ml]和视觉模拟评分（VAS）[（3.9±0.6）分]均低于胸腔镜辅助组[（258.0±48.6）ml、（6.5±1.2）分],差异均有统计学意义（t=3.75、12.96,均P＜0.05）。全胸腔镜组患者术后生存质量评分[总分（88.7±9.6）分]高于胸腔镜辅助组[总分（71.1±8.2）分],差异有统计学意义（t=9.1,P＜0.05）。两组患者术后无瘤生存时间比较差异无统计学意义（P＞0.05）。结论全胸腔镜手术治疗Ⅰ期非小细胞肺癌的近、远期疗效均较好,值得推广应用。     

Synthesis of a flavonoid fluorescent probe for the detection of sulfur dioxide derivatives and cell imagingEI北大核心CSCD

A novel fluorescent probe HMQC was synthesized for HSOf detection by coupling flavonoid derivatives with 3-quinoline salt. In PBS buffer solution, the probe showed high selectivity, good sensitivity (58 nmol/L) and rapid response (150 s) for the detection of HSO3−. The possible sensing mechanism of the probe was discussed by nuclear magnetic hydrogen spectroscopy, mass spectrometry and theoretical calculation, indicating that the addition reaction between HSO3− and the C=C bond of the probe led to the fluorescence enhancement. The probe HMQC could be used for the detection of HSO3− in living cells, making it to be a promising tool for delecting HSO3−. © 2023, Youke Publishing Co.,Ltd. All rights reserved.     

Synthesis,Crystal Structure,and Cytotoxic Property of Dibromobis{4-bromo-2-[（2- phenylaminoethylimino）methyl]phenolato}dizinc（II）

The title phenolic oxygen bridged dinuclear Schiff base zinc（II） complex [Zn2Br2- （C15H14BrN2O）2] was synthesized and characterized by elemental analysis and single-crystal X-ray diffraction. The crystal belongs to the triclinic system, space group P1 with a = 9.2279（17）, b = 12.096（2）, c = 16.286（3） A, α = 75.402（3）, β = 87.464（3）, γ= 72.746（3）°, V = 1679.1（5） A^3, Z = 2, Dc = 1.833 g/cm^3, Mr = 926.94, λ（MoKα） = 0.71073 A, μ= 6.221 mm^-1, F（000） = 904, the final R = 0.0601 and wR = 0.1155. A total of 7453 unique reflections were collected, of which 3425 with I 〉 2σ（I） were observed. One Zn atom is six-coordinate in an octahedral geometry, and the other one is four-coordinate in a tetrahedral geometry. The Zn...Zn distance is 3.106（2） A. In the crystal structure, molecules are linked through intermolecular hydrogen bonds typed N-H...Br and C-H...Br, forming zigzag chains running along the b axis. The complex shows potent cytotoxic property to both normal and carcinoma cells.     

含氮药物分子中氮a-位碳氢键离解能的理论研究

本文利用G3B3 和CBS-Q高精度理论方法检验了一系列胺类有机化合物中α-碳氢键离解能的实验测量值,在此基础上筛选出(U)BHandH/6-311++G(2df, 2p)//(U)B3LYP/6-31G(d)方法,发现其可以准确快速的预测氮α-碳氢键离解能。运用该方法研究了若干含氮药物分子,发现氮α-碳氢键离解能随药物分子结构发生明显变化。为了阐明其变化规律,系统研究单取代和双取代基效应,并解释了不同取代基效应的来源。     

Synthesis and biological evaluation of nitric oxide-releasing sixalkoxyl biphenyl derivatives as anticancer agents

A series of novel nitric oxide-donating sixalkoxyl biphenyl derivatives （14a-1） were synthesized by coupling furoxan with alkoxyl biphenyl skeleton using amino acids as the spacers, and their cytotoxicity against HepG2 cells in vitro were evaluated by MTT method. It was found that 14c, 14d, 14f, 14i, 14j and 14k showed more potent cytotoxic activities than control 5-fluorouracil. NO release assay of target compounds indicated that the maximum amount of NO released by most active compounds 14c and 14j was about 6 × 10^-2 μmol/L, whereas 14a and 14h with very weak activity only released NO of 1 × 10^-2 μmol/L.     

Synthesis and in vitro cytotoxicity of novel 1,4-disubstituted phthalazines

Eight novel 1,4-disubstituted phthalazines （7-14） were designed and synthesized. The structures of all the synthesized compounds were confirmed by IR,1H NMR, 13C NMR, MS and elemental analysis. Their in vitro cytotoxicity against cancer cell lines （Bel-7402 and HT- 1080） were evaluated by standard MTT assay. Among them, compounds 9 and 11 exhibited more potent cytotoxicity than cisplatin. 2007 Ping Gong. Published by Elsevier B.V. on behalf of Chinese Chemical Society. All rights reserved.     

烷基酚类死亡相关凋亡诱导蛋白激酶2 (DRAK2)小分子抑制剂的设计与合成研究

死亡相关凋亡诱导蛋白激酶2 (DRAK2)为苏氨酸/丝氨酸激酶,是死亡相关蛋白激酶(DAPK)家族中的一员,在外界刺激的作用下,其过表达具有诱导细胞凋亡的能力.已知高糖、高脂和某些细胞炎性因子均可诱导DRAK2的高表达而引起胰岛β细胞凋亡,提示DRAK2可能在其中扮演着重要的介导作用,因此靶向DRAK2的小分子抑制剂可...     

一种水溶液中裸眼识别HSO3-/SO32-的长波长荧光探针及其应用

合成了一种(E)-2-(3-氰基-4-(3-甲酰基-4-羟基苯乙烯基)-5,5-二甲基呋喃-2(5H)-亚烷基)丙二腈衍生物CDM,1H NMR、13C NMR 和 HRMS 验证了其结构.CDM 在 MeCN/Tris(V:V=2:8,pH = 7.4)溶液中通过荧光信号ON-OFF识别HSO3-/SO3-,颜色...