驱虫斑鸠菊化学成分的研究

从驱虫斑鸠菊(Vernonia anthelmintica Willd)的种子中分离到十个化合物, 经理化性质及波谱分析鉴定为斑鸠菊黄烷苷-对羟苯甲酯(p-Hydroxy benzoyl-Vemovan)(1)、斑鸠菊黄烷苷(Vernovan)(2), 为未见文献报道新化合物, 并发现斑鸠菊大苦素(Vernadalin)(3)、斑鸠菊醇(Vernodalol)(4), 对P388白血病细胞有抑制作用,5,6,7,8及10为该属植物中首次获得。     

硒化壳聚糖对NB4细胞作用的实验研究

应用MTT法、AO/EB荧光染色法和NBT还原法观察硒化壳聚糖对NB4肿瘤细胞株生长的影响。结果发现,硒化壳聚糖可有效地抑制NB4细胞生长,并呈量效、时效关系。经100 mg/L及以上剂量硒化壳聚糖作用后的细胞可明显出现核固缩、碎裂等凋亡形态改变。而经100 mg/L以下剂量硒化壳聚糖作用后,细胞可出现诱导分化成熟的表现。     

色酮吡唑啉酮类衍生物的合成及抗白血病细胞活性

以色酮-吡唑啉酮作为C1合成子, 在5 mol% 的DBU催化下,与α,β-不饱和酮发生Michael加成反应, 获得了10个色酮吡唑啉酮类衍生物3a~3j,产率76%~90%, dr值为4/1~9/1, 其结构经¹H NMR, ¹³C NMR和HR-MS（ESI-TOF）表征,通过单晶进一步进行了确定化合物3b的相对构型。该类化合物包含有连续两个立体中心,包括一个季碳中心,可以为生物活性筛选提供物质基础。采用MTT法研究了3a~3f对人白血病细胞(K562)的体外抗增殖活性。结果表明：化合物3a, 3c, 3d和3i对K562增殖具有一定的抑制活性。     

CD4+T细胞亚群在MDS与AML中的变化比较和临床意义

目的通过比较骨髓增生异常综合征和急性髓系白血病患者Thl、Th2、Thl7和Treg细胞的数量变化,以观察两种疾病的细胞免疫状态,分析其临床意义。方法采用流式细胞仪检测难治性贫血组(RA)20例,难治性贫血伴原始细胞增多组(RAEB)25例;AML组15例患者外周血中Thl、Th2、Thl7和Treg细胞的水平,采用SPSS 13.0统计学软件进行数据分析处理。结果相较于正常对照组,Th1、Th17细胞和Th1/Th2在MDS-RA组均升高(P<0.05),MDS-RAEB组和AML组均减低(P<0.05);Th2细胞比例MDS-RA组减低(P<0.05),MDS-RAEB组和AML组升高(P<0.05);而Treg细胞MDS-RA组比例无统计学意义(P>0.05),MDS-RAEB组和AML组升高(P<0.05)。相较于MDS-RA组,MDS-RAEB组Th1、Th17细胞比例和Th1/Th2减低(P<0.05),而Th2和Treg细胞比例升高(P<0.05)。与MDS-RAEB组相比,AML组Th1细胞比例减低(P<0.05),Th2和Treg细胞升高(P<0.05),而Th17细胞和Th1/Th2无统计学意义(P>0.05)。结论 Th细胞向Th1型细胞极化,Th17细胞显著性升高为主要表现的Th1/Th2细胞因子网络的破坏使在MDS的早期阶段产生过多的造血抑制因子可能是造血功能衰竭的主要原因;Th细胞向Th2型细胞极化,Th17细胞减低,Treg细胞比例增高在MDS晚期阶段及AML中引起异常克隆细胞的积聚可能是造血功能衰竭的主要原因。     

1-取代哌啶-4-酮芳甲酰腙的合成及其抗白血病活性

以取代苄胺或伯胺为原料,依次经过Michael加成、Dieckmann缩合、水解脱羧、酰腙化反应合成了10个未见文献报道的1-取代哌啶-4-酮芳甲酰腙9a～9j,化合物结构经1H NMR,IR,MS和元素分析确证.初步的生物活性测试表明,部分化合物能有效抑制白血病K562细胞的增殖,具有潜在的抗白血病活性.     

Synthesis and Anti-leukemia Evaluation of Tetrahydro-4H-pyrano[3,2-c]pyridines and Corresponding Anti-CD14 Monoclonal Antibody Conjugates

A series of novel tetrahydro-4H-pyrano[3,2-c]pyridines（3a-3p） were synthesized and found to possess potent antiproliferative activity against leukemia K562 cells in vitro. Preliminary bioassay indicates that compounds 3a and 3e afford the best activity, the IC50 values of them were 6.93 and 7.51μg/mL, respectively, which were lower than that of the anticancer drug 5-FU（1C50=8.56μg/mL）. To reduce the toxicity of compounds 3a-3p to the prolife- ration of normal bematopoietic cells, a tumor targeted CD14 monoclonal antibody（McAb） was used in conjugation with compounds 3a--3p to get conjugates 4a--4p, respectively. The inhibitory activities of conjugates 4a--4p toward K562 cells were discovered to approach those of compounds 3a--3p. In the presence of CD14 McAb, tumor cells were found to be much more susceptible to conjugates 3a--3p than normal hematopoietic cells. Therefore, the toxicity of conjugates 4a--4p to normal hematopoietic cells declined obviously. For example, as for the toxicity of compound 3a compared with that of compound 4a, the value of ICs0 increased from 35.90 μmol/L to 39.52 μmol/L.     

Electrochemical study of the effect of functionalized nickel nanoparticles on cellular uptake of leukemia cancer cells in vitro

In this study, we have fabricated the functionalized nickel nanoparticles and investigated their effects on cellular uptake of quercetin in leukemia K562 cancer cells by using electrochemical assay. The results indicate that nickel nanoparticles could efficiently enhance the quercetin uptake and increase the intracellular accumulation in cancer cells, implying the great potential of functionalized nickel nanoparticles in target cancer therapy.     

cDNA Cloning, Prokaryotic and Eukaryotic Expression and Characterization of Porcine Leukemia Inhibitory Factor

Introduction Theleukemiainhibitoryfactor(LIF)isamulti functionalcytokinebelongingtotheinterleukin6(IL6)family[1].Itwasfirstfoundasamyeloidleukemic cellproliferationsuppressoranddifferentiationinducer cytokine[2].ThenaturalformofLIFisa38to67kDa glycosylate…     

硒代二半胱氨酸对白血病细胞系的诱导分化研究

硒抑制体外白血病细胞的生长和增殖．并能诱导部分白血病细胞分化成熟。但其药理作用因硒制剂的不同而异．硒代二半胱氨酸抑制V937和K562血病细胞的生长和增殖，半数抑制浓度为3．0μmol／L，经30μmol／L硒代二半胱氨酸作用3天后，U937胞吞噬率以5％上升至14％，K562细胞内血红蛋白含量由0．20增至0．40μg／10^6细胞，说明硒代二半胱氨酸能诱导部分白血病细胞分化成熟。     

高效液相色谱法测定超滤后血清中的假尿苷

用高效液相色谱法（ＨＰＬＣ）直接测定了超滤后血清中的假尿苷（ＰＳＥ）,平均回收率为９７．７８％,在０．０９３～６μｍｏｌ范围内呈线性关系（γ＝０．９９５６）,急性白血病患者血中ＰＳＥ水平显著高于正常对照组（Ｐ＜０．０１）。该方法简单、方便、迅速,可用于恶性病的病情及疗效监测。