人X染色体单拷贝序列的分离及鉴定的研究

本研究以人/鼠(CHO-K1)细胞总DNA为探针对人X染色体DNA文库进行了3轮筛选,单拷贝顺序的检出率为1.45％。其中DXFD52,71,73,75分别与一组含人X染色体及不含人X染色体的人/鼠杂种细胞DNA进行Southern杂交分析,确定DXFD52,71,73,75含人X染色体专性DNA顺序。采用染色体原位杂交的方法,将DXFD52精确定位于Xq12-q13。并对其进行了部分DNA顺序分析,结果证实DXFD52是人基因组中至今未被分离到的一个单拷贝DNA片段。继而对DXFD52进行限制性酶切图谱分析,并以其为探针在中国重庆地区正常人群中(随机个体38例、3个正常家系成员11例)进行了RFLP研究,发现该探针具有识别Hind Ⅲ,Bgl Ⅱ,Hinf Ⅰ的RFLP。     

XPS研究电抛光铜表面粘液膜的组成

金属的电抛光应用越来越广泛.但电抛光机理至今仍不很清楚.我们曾用 XPS 和AES 研究了不同槽电压下电抛光铜表面的组成和物种,确认其表面均为纯铜,不存在含磷固体膜.本文用 XPS 研究电抛光液中1-羟基乙叉-1,1-膦酸(HEDP)浓度、抛光液的 pH 及其接受体(H_3PO_4,HEDP 和 HEDP H_3PO_4)变化时铜的表面组成,并进一步证明,电抛光时形成的粘液膜是水溶性膜,铜表面在不同抛光条件下并不形成难溶的含磷固体膜.通过电子能谱对固化后粘液膜组成和深度分布的研究确定,粘液膜可能是铜的多核聚合混合配体配合物,其组成可近似地表示为[Cu_4(PO_4)(HEDP)]_n.     

生物系统中活泼中间体与脂质过氧化

述评了生物系统中引起脂质过氧化的活性物质之形成、脂质过氧化的反应中有关活泼中间体的问题。     

PVC膜电极上的示波电位滴定法及其在药物分析中的应用

本文研究了用于示波电位滴定的PVC膜电极,成功地用于中和、络合及沉淀滴定,并探讨了它们在药物分析中的应用。所研究电极见表1。实验部分一仪器与试剂 SR-071型双踪示波器,213型铂片电极     

中药川白芷化学成分的研究

中药川白芷为伞形科当归属杭白芷 (Ａｎｇｅｌｉｃａｄａｈｕｒｉｃａ (Ｆｉｓｃｈ .ｅｘＨｏｆｆｍ .)Ｂｅｎｔｈ .ｅｔＨｏｏｋ .ｃｖｈａｎｇ ｂａｉｚｈｉＨｏｒｔ)的干燥根。川白芷为我国传统中药 ,主治头疼、鼻炎、癣症等疾病。现代药理实验表明白芷中所含的呋南香豆素具有平喘、降压、抗菌、解痉、光敏、活化交感系激素等多种药理作用[1] 。已有文献对白芷的化学成分进行了研究 ,发现白芷中主要含香豆素成分[2～ 4] 。四川遂宁产川白芷为道地白芷 ,其药性好 ,为进一步研究其活性成分 ,本文对遂宁产川白芷进行化学成分研究。共分离、…     

A New Sesquiterpene Lactone from Ainsliaea bonatii

A new sesquiterpene lactone, Ainsliaolide A, was isolated from Ainsliaea bonatii. The structure was determined on the basis of spectral data.     

穿过液膜的自发电势振荡

An artificial membrane consisting of pricramic acid in nitrobenzene was used as an oil phase imposed between two aqueous phases. The right one contained trimethyl-slearylammonium chloride and aicohol; and the left contained sucrose solution (or H_2O). We found that this system exhibited rhythmic oscillation of electrical potential. The amplitude of the oscillations was between 20—150 mV. The oscillations were observed even when ethyl alcohol was absent in the aqueous of trimethylslearylammonium Chloride, and the increase in amplitude of oscillation with time was found to be less in the presence of sucrose. Some of the expremental results have been explained.     

手性配体立体选择性萃取分离扁桃酸对映体

Based on chiral ligand exchange, the distribution behavior of mandelic acid enantiomers in the two-phase system containing copper ion(Ⅱ)(Cu²⁺) and N-n-dedecane-L-hydroxyproline (L), was studied. At different pH, the partition of Cu²⁺ in the two-phase system containing N-n-dedecane-L-hydroxyproline, was investigated. The formation constant of binary cpmplex(Li₂Cu) was obtained by regression. The influence of pH, concentrations of Cu²⁺ and chiral ligand, and solvents on partition coefficients(K) and separation factor(α), was discussed. The experimental results show that Li forms more stable ternary complex with D-mandelic acid than with L- mandelic acid. There is a large influence of pH on K and α. At low pH values (<3.5), formation of binary complexes is thermodynamically unfavourable. K and α are best when pH values are above 3.5 and the molar ratio of chiral ligand to Cu²⁺ is 2∶1. At the same time, solvents influence K and α very much.     

聚醚型非离子表面活性剂的混合表面膜III, 环氧乙烷一环氧丙烷嵌段共聚物, 聚丙二醇与十六醇, 硬脂酸的二元混合物

本文进一步研究了环氧乙烷-环氧丙烷嵌段共聚物(L61和F68)、聚丙二醇(PPG)分别与十六醇(CA)、硬脂酸(SA)所形成的二元混合膜。根据对π-α实验结果的分析, 表明PPG-CA、PPG-SA、F68-SA和L61-SA的混合单分子膜是不相混溶的, 而L61-CA和F68-CA的混合单分子膜则是混溶的。