盐酸小檗碱经海藻酸/大豆蛋白复合微球负载后的释放行为

利用海藻酸(AL)/大豆蛋白(SPI)复合微球包裹盐酸小檗碱(Ber), 研究了盐酸小檗碱经复合微球负载后在模拟胃,小肠和结肠pH环境下的释放行为.结果显示,在模拟肠和结肠的pH 6.8和pH 7.4环境中,约5 h盐酸小檗碱就分别达到96%和99%的药物溶出量,而在模拟胃的pH 1.0条件下溶出量仅为约40%.研究发现盐酸小檗碱经复合微球负载后的释放行为与复合微球在不同pH条件下的溶胀行为直接相关,即在pH 1.0时溶胀度很小,但在pH 6.8和pH 7.4条件下溶胀度可达到约2000%.这主要归功于海藻酸组分的pH敏感性.同时,负载过程使结晶的疏水盐酸小檗碱转变为更易于在水性介质中扩散和溶解的不定型态.特别是通过pH梯度实验发现,限定载药复合微球在模拟胃的pH 1.0溶液中的时间在3 h内,可控制盐酸小檗碱的溶出量为约20%;而在此后的小肠和结肠的pH条件下,盐酸小檗碱迅速溶出,使累积溶出量达到98%.由此可见,利用自主研制的复合微球负载盐酸小檗碱可以降低盐酸小檗碱对胃部的刺激并可应用于盐酸小檗碱对溃疡性结肠炎的治疗,同时大豆分离蛋白的生理活性可望发挥一定的辅助作用.     

Synthesis and biological evaluation of a new series of histone deacetylases inhibitors

Histone deacetylases （HDACs） play an important role in tumorigenesis. Inhibition of HDACs is considered as a potent strategy for cancer therapy. Two lead compounds （ja and jb） were found to have activities against HDACs with IC50 at about 15 μmol/L. Then a new series of hydroximic acid derivatives were designed and synthesized based on them. The HDACs activity assay in vitro found that compounds J04 and ,109 are nearly as potent as the positive control drug Zolinza.     

光热偏转谱术

本文介绍一种基于光声（热）原理的光吸收测量新技术．当某调制光被物质吸收时会产生热量，因而被测样品及周围介质巾产生相应的温度场．当探测光掠过或穿过此温度场时将被偏转．用位置灵敏探测器接锁相放大器测出探测光的偏转量，从而计算出样品的微弱吸收．此法比现有常用的透射法灵敏度高３—６个数量级，是研究薄膜材料光热性能十分重要的手段．     

大剂量补充微量元素治疗“胃溃疡型腺癌伴广泛转移”一例

阐述了一例胃底溃疡型腺癌伴周边器官淋巴组织广泛转移患者，行“贲门MT术”姑息治疗后，经九个月大剂量多种微量元素制剂补充达到很好的临床效果。     

含黄酮的三价铁配合物合成及其药理性质(英文)

3,3,4,7-四羟基黄酮与1,3-二环己基碳二亚胺在4-二甲胺吡啶存在下反应,得到2,2-双(3,3,4,7-四羟基黄酮)1,3-二环己基碳二亚胺。三价金属离子与上述合成得到的配体螯合,对此四面体结构的双核Fe(Ⅲ)配合物的血液学及病理学数据进行了讨论,同时研究了合成的配体作为螯合剂清除鼠肝胰组织中超载的铁的能力。结果表明作为口服药其作为双齿配体的螯合能力与市售用于治疗地中海贫血的药物"去铁敏"相近。     

Differential Expression of Molecular Chaperones in PC12 Cells Treated with PSI

<正>Parkinson's disease(PD) is a common neurodegenerative disorder whose primary pathology features are the degeneration of dopaminergic neurons in the substantia nigra pars compacta(SNc) and the presence of eosinophilic inclusions called Lewy body in the cytoplasm of the remained neurons.Growing evidence suggests that dysfunction of the ubiquitin-proteasome system(UPS) is involved in the etiopathogenesis of PD.In order to investigate the pathogenetic mechanism of ubiquitin-proteasome dysfunction in PD,2D-differential gel electrophoresis (2D-DIGE) and MALDI-TOF Pro MS were used to determine the proteins,which were differentially expressed,in PC12 cells that had undergone a synthetic proteasomal inhibitor PSI(10μmol/L) treatment for 24 h.Forty-six protein spots were differentially expressed in response to PSI administration,of which 34 were increased and 12 decreased. Six of these were identified as molecular charperones:endoplasmin precursor(GRP94),heat shock protein 105(HSP105),HSC-70-psl,glucose ruglated protein 75(GRP75),glucose ruglated protein 58(GRP58) and heat shock 27000 protein 1(HSP27).The results suggest that the molecular chaperones play an important role in the PD model induced by proteasomal inhibitor.     

Preparation of Spherical Silica-supported Biosorbent for Copper Ions Removal in Wastewater Based on Sol-gel Reaction and Simple Treatment with Sodium Hydroxide

A new and effective strategy was proposed for preparing new organic-inorganic composite biosorbent with spherical silica as supporting core and chitosan(CS)-based hybrid layer as shell based on sol-gel reaction and simple treatment with sodium hydroxide(NaOH). The coating layer was covalently bound on the supporting silica through polysaccharide incorporated sol-gel process starting from CS and inorganic precursor γ-glycidoxypropyl-trimethoxysiloxane(GPTMS). GPTMS had epoxide groups and cross-linked amine groups of CS to avoid its acidic solubilization. The composite biosorbent had coarse surface due to the wet phase-inversion by treating in NaOH solution. The prepared biosorbent could be used in treating electric plating wastewater.     

适配体在癌症诊断与靶向治疗中的研究进展

核酸适配体是利用体外筛选技术,即指数富集的配体系统进化技术(SELEX),从核酸分子文库中得到的寡核苷酸片段。其与靶标物有很高的特异性和亲和力,将适配体作为识别单元的生物传感研究以及适配体偶联成像试剂的生物体内外成像研究在临床诊断中有很大的应用前景,此外,适配体靶向癌细胞或组织的治疗方法相比传统化学治疗副作用更小,在临床上也有极大的应用前景。本文综述了适配体目前在癌症诊断和靶向治疗两个方面的研究进展,并分析现阶段存在的问题以及面临的挑战。     

癌症、肿瘤抑制基因及纳米技术在癌症预防和治疗的应用

 一、什么是癌症
癌症（cancer）是发生于各个年龄段、多种器官和组织、导致严重后果的疾病。根据世界卫生组织的统计,2007 年全世界有790 万人死于癌症,占所有死亡人数的13%,据估计到2030 年该数字将上升至1200 万。每年花在癌症病人治疗和护理的费用超过2000 亿美元,给社会和家庭带来了巨大的经济负担。根据细胞类型,癌症可以分为四类,内外表层细胞发生癌变形成的肺癌、乳腺癌和结肠癌等（Carcinoma）;支持组织和连接组织,如骨、软骨、脂肪和肌肉等形成的肉瘤（Sarcoma）;淋巴和免疫系统形成的淋巴瘤（Lymphoma）;以及循环系统形成的白血病（Leukemia）。     

基于压电探测判定激光支持燃烧波和爆轰波的点燃阈值

为了获得激光支持燃烧波和爆轰波的点燃阈值,采用压电探测器检测波长为1 064 nm的Nd:YAG激光作用在铝靶表面所产生的应变和冲压。从实验结果观察到压电信号的变化分为3个阶段,分别为光热弹性应变阶段、等离子体增强耦合阶段和激光支持爆轰波对靶表面的压力阶段,并从理论上研究了这3个阶段的激光与靶材料相互作用的机理,从而可以从压电信号是否发生跃变判断出激光支持燃烧波和激光支持爆轰波的点燃阈值,与其它方法所得到结果基本吻合。