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William H. Newsome J. Brian Shields 《International journal of environmental analytical chemistry》2013,93(3-4):295-304
A radioimmunoassay was developed capable of determining Aroclor 1260 in milk at levels of from 20 to 80 ppb and in blood from 2 to 16 ppb. The values obtained by radioimmunoassay correlate well with those determined by gas-liquid chromatography (r2 = 0.96 for milk and 0.99 for blood) but were lower. Antiserum was produced in rabbits and was specific for 2, 2′, 4, 4′, 5, 5′-hexachlorobiphenyl. It cross-reacted with congeners and isomers in Aroclor 1254 and 1260 to the extent that a 20% decrease in binding was observed with 0.1 ng of either mixture. The method requires preliminary cleanup of the extract on alumina and utilizes 25 % dimethyl sulfoxide in the assay medium to promote solubilization of the substrates. 相似文献
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D. Neumeier R. Hofstetter und B. Glück 《Fresenius' Journal of Analytical Chemistry》1978,290(2):176-177
Ohne Zusammenfassung
Radioimmunoassay for subunit B of creatine phosphokinase: Preliminary results pertaining to concentration kinetics after myocardial infarction相似文献
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M. V. Sofroniew M. Madler O. A. Müller P. C. Scriba 《Fresenius' Journal of Analytical Chemistry》1978,290(2):163
Conclusion The high success ratio of this method for production of high quality antisera against several small peptides, suggests that this method may be useful for other small antigens. Successful preliminary results with prostaglandine E and thyroxine support this assumption.Supported by the Deutsche Forschungsgemeinschaft (grant We608/3 and SFB 51) 相似文献
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《Analytical letters》2012,45(12):2587-2594
Abstract Polyspecific antiserum against total rat serum proteins was used to develop specific and sensitive radioimmunoassays for fetuin and bikunin, two minor protein components of rat plasma. The radioimmunoassays proved to be highly useful to trace bikunin and fetuin in the course of developing isolation procedures, since neither specific functional assays nor monospecific antisera were available. The two examples demonstrate that, in general, it will be possible to develop a specific and sensitive radioimmunoassay with antiserum raised against a crude antigen preparation, such as a body fluid or a tissue extract, provided that a minute amount of pure antigen is available for preparing the radioiodinated antigen. 相似文献
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P. Sandel W. Vogt B. Popp und M. Knedel 《Fresenius' Journal of Analytical Chemistry》1976,279(2):126-127
Ohne Zusammenfassung
Smoothing-spline and polygonal interpolation, logit-log-linear- and logit-log-parabolic-regression: A comparative study of mathematical methods for the calculation of radioimmunoassay-results相似文献
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Ohne Zusammenfassung
A new 125I-anti-DNA-radioimmunoassay for the diagnosis of systemic lupus erythematosus相似文献
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Günter Knapp Brigitte Maichin und Hans Spitzy 《Fresenius' Journal of Analytical Chemistry》1979,295(5):402-405
Zusammenfassung Eine radioimmunologische Methode zur simultanen Bestimmung der beiden Schilddrüsenhormone T3 und T4 im Blutserum wird beschrieben. Die Abtrennung der Hormone von den Serumproteinen, die Reaktion mit dem Antiserum und die bound-free-Trennung erfolgen auf kleinen lonenaustauschersäulen. Als Säulenfüllung dient ein Mischbettaustauscher, bestehend aus QAE- und SP-Sephadex. Die Analysenergebnisse sind mit der G-25 Säulenmethode vergleichbar. Der Materialaufwand bei Einmalsäulen kann durch die Verwendung des beschriebenen Ionenaustauschers stark gesenkt werden.Herrn a.o. Univ.-Prof. Dipl.-Ing. Dr. techn. Wladimir Limontschew zum 60. Geburtstag 相似文献