无花果多糖的分离、纯化和鉴定

经脱脂、沸水抽提、乙醇沉淀、酶-Sevag法脱蛋白得到粗多糖,再经DEAE-Sepharose fast flow阴离子交换层析和Sephadex G-200葡聚糖凝胶柱层析得到纯化的无花果多糖(FCPS),用红外光谱和紫外光谱分析鉴定该多糖.红外光谱分析具有典型的多糖特征吸收峰,紫外光谱分析未见蛋白质(280 nm)与核酸(260 nm)的特征吸收峰.结果表明,所提取的无花果多糖为单一组分的多糖.     

Survey of Phenolic Acids,Flavonoids and In Vitro Antioxidant Potency Between Fig Peels and Pulps: Chemical and Chemometric Approach

In the present study, chromatic coordinates, phenolic acids, flavonoids and antioxidant capacity assessed by 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonate (ABTS) and lipid peroxidation inhibition capacity (LPIC) essays and their relative IC50 were investigated in 25 fig cultivars growing in Morocco. The aims of this study were to determine (i) the variation in these compounds among light and dark-colored cultivars, (ii) their partitioning between fruit peel and pulp and (iii) to display network connections among these variables. Twelve phenolic compounds (PCs) were isolated in peel extract versus eight in pulp samples. Anthocyanins, mainly cyanidin-3,5-diglucoside and cyanidin-3-O-rutinoside, were the predominant compounds in peels, where the mean concentrations were 75.90 ± 18.76 and 77.97 ± 18.95 µg/g dw, respectively. On the other hand, (−)-epicatechin and cyanidin-3-O-rutinoside were the major compounds in the pulp extracts, where the mean values were 5.23 ± 4.03 and 9.01 ± 5.67 µg/g dw, respectively. A two-dimensional hierarchically clustered heatmap was applied to the dataset to explore correlations in the dataset and similarities between cultivars, without dimensionality reduction. Results showed that anthocyanins, particularly pelargonidin-3-O-rutinoside, cyanidin-3,5-diglucoside and cyanidin-3-O-rutinoside, were the main contributors to the peels’ free radical scavenging capacity. This capacity was particularly higher in the peel of dark-colored figs compared to the fruit pulp. The local cultivar “INRA 1301” showed the most promising phenolic profile due to its very high levels of almost all detected PCs, especially (−)-epicatechin, quercetin-3-O-rutinoside, quercetin-3-O-glucoside, cyanidine-3,5-diglucoside, cyanidine-3-O-rutinoside and pelargonidin-3-O-rutinoside (54.66, 141.08, 35.48, 494.08, 478.66, 12.56 µg/g dw, respectively). Having the darkest figs in the collection (L* = 25.72, c* = 22.09 and h° = 20.99), this cultivar has also combined promising IC50 values, which were of 19.85, 40.58 and 124.78 µg/mL for DPPH, ABTS and LPIC essays, respectively.     

Non‐Alkaloidal Constituents from the Stem of Ficus Septica

Seven triterpenes, two lignans, and twelve other known compounds were isolated from the non‐alkaloidal fractions of the stem of Ficus septica Among these triterpenoids, a rare triterpene skeleton derivative, 13,27‐cycloursan‐3β‐ylacetate was isolated from natural sources for the first time. All the structures of these compounds were elucidated by spectroscopic methods.     

Alkaloid extracts of Ficus species and palm oil-derived tocotrienols synergistically inhibit proliferation of human cancer cells

Tocotrienols have been reported to possess anticancer effects other than anti-inflammatory and antioxidant activities. This study explored the potential synergism of antiproliferative effects induced by individual alkaloid extracts of Ficus fistulosa, Ficus hispida and Ficus schwarzii combined with δ- and γ-tocotrienols against human brain glioblastoma (U87MG), lung adenocarcinoma (A549) and colorectal adenocarcinoma (HT-29) cells. Cell viability and morphological results demonstrated that extracts containing a mixture of alkaloids from the leaves and bark of F. schwarzii inhibited the proliferation of HT-29 cells, whereas the alkaloid extracts of F. fistulosa inhibited the proliferation of both U87MG and HT-29 cells and showed synergism in combined treatments with either δ- or γ-tocotrienol resulting in 2.2–34.7 fold of reduction in IC₅₀ values of tocotrienols. The observed apoptotic cell characteristics in conjunction with the synergistic antiproliferative effects of Ficus species-derived alkaloids and tocotrienols assuredly warrant future investigations towards the development of a value-added chemotherapeutic regimen against cancers.     

Caprine herpesvirus 1 (CpHV-1) vaginal infection of goats: clinical efficacy of fig latex

Clinical score (A) and viral shedding (B) in goats vaginally infected with CpHV-1 and treated with fig latex (goats A, B, C). Goat D was left untreated.     

New Prenylated Flavones from the Roots of Ficus Beecheyana

Two new prenylated flavanones, ficubee A and ficubee B, respectively, as 7,8‐(2,2‐dimethylpyrano)‐6‐prenyl‐5,3′,4′‐trihydroxyflavone and 6,7‐(2,2‐dimethylpyrano)‐8‐prenyl‐5,3′,4′‐trihydroxyflavone were isolated from the roots of Ficus beecheyana together with twelve known compounds: β‐sitosterol, 5‐stigmasten‐3β,7α‐diol, 5‐stigmasten‐3β,7β‐diol, 3β‐hydroxystigmast‐5‐en‐7‐one, 4‐hydroxybenzaldehyde, 4‐hydroxy‐3‐methoxybenzaldehyde, 1‐(4‐hydroxyphenyl)‐ethanone, 4‐hydroxy‐3‐methoxybenzoic acid, 4‐hydroxy‐cinnamic acid, seseline, xanthyletin, and psoralene. The structures of these secondary metabolites were determined by spectroscopic means and in comparison with published data.     

Investigation of antiplasmodial efficacy of lupeol and ursolic acid isolated from Ficus benjamina leaves extract

Abstract

This study emphasizes on the investigation of antiplasmodial activity of triterpenoids isolated from Ficus benjamina leaves. An unsaponified fraction of petroleum ether extract of plant leaves was subjected to silica gel column chromatography which led to the isolation of two known triterpenoids; namely ursolic acid and lupeol. These compounds were evaluated for antiplasmodial activity by schizont maturation inhibition assay using 3D7 Plasmodium strains. Both, ursolic acid and lupeol were found to exhibit significant antiplasmodial effect with an IC₅₀ value of 18?µg/ml and 3.8?µg/ml, respectively. This study further confirms the traditional role of Ficus benjamina plant in the treatment of malaria which may be attributed to ursolic acid and lupeol.     

琴叶榕根中微量元素分析

为了深入探讨琴叶榕根提取液治疗细菌性前列肠炎与其微量元素之间的关系，全面了解琴叶榕根药理机制，采用火焰原子吸收分光光度法测定了琴叶榕根中Zn、Fe、Mn、Cu、Cr五种微量元素的含量。测定结果表明，在琴叶榕根中微量元素的含量顺序为Zn＞Mn＞Fe＞Cu＞Cr。琴叶榕根提取液治疗细菌性前列肠炎不仅与其有机成分有关，还与所含微量元素有关。     

A new benzofuran derivative from the leaves of Ficus pumila L.

A new benzofuran derivative, pumiloside (1), together with seven known flavonoid glycosides, afzelin (2), astragalin (3), quercitrin (4), isoquercitrin (5), kaempferol 3-O-rutinoside (6), rutin (7) and kaempferol 3-O-sophoroside (8) were isolated from the leaves of Ficus pumila. Their structures were established by spectroscopic data and comparison with the literature values.     

A new helvolic acid derivative from an endophytic Fusarium sp. of Ficus carica

A new helvolic acid derivative named helvolic acid methyl ester (1), together with two known helvolic acid compounds, helvolic acid (2) and hydrohelvolic acid (3), were isolated from the fermentation of endophytic fungus Fusarium sp. in Ficus carica leaves. Their structures were elucidated and identified by spectroscopic methods. Compounds 1–3 showed potent antifungal and antibacterial activities.