固态碳糊电极电位法测定利多卡因注射液

本文报道了一种以利多卡因与单质碘形成的缔合物为电活性物的全固态碳糊利多卡因电极,电极的线性响应范围5.0×10-2～4.0×10-5mol.L-1,级差电位为30mV/pC,检测下限为3.0×10-5mol.L-1。该电极响应迅速,重现性好,用该电极测定了盐酸利多卡因注射液中利多卡因的含量,结果与药典法相符。     

分光光度法测定替米沙坦

用分光光度法研究了替米沙坦与茜素的荷移反应,提出了测定替米沙坦的荷移分光光度法。在丙酮溶剂中,替米沙坦和茜素形成的络合物的最大吸收波长为548nm,两者的组成比为1比2。替米沙坦质量浓度在16～240mg·L-1范围内服从比耳定律,表观摩尔吸光率为2.34×103L.mol-1.cm-1。采用此法分别测定了替米沙坦胶囊和片剂中替米沙坦的含量,所得测定值与文献报道值相符。用标准加入法做方法的精密度和回收试验,测得回收率为99.5%(胶囊)和99.8%(片剂)。     

Sonocatalytic damage of solute bovine serum albumin by disperse ZnO/porcine dens composite under ultrasonic irradiation

ZnO/Dens composite were prepared with nano-sized ZnO and porcine dens powders by mechanical mixing, liquid boiling, ultrasonic dispersion and heat-treated at 500°C for 50 min. The samples were characterized by X-ray diffraction (XRD) and its sonocatalytic activity was evaluated by the damage of bovine serum albumin (BSA). Furthermore, the effects of ultrasonic irradiation time, ZnO/Dens composite amount, solution acidity and ionic strength on the sonocatalytic damage of BSA were evaluated. The results show that the damage degree of BSA aggravated with the increase of ultrasonic irradiation time, ZnO/Dens composite amount and ionic strength, but weakened with the increase of solution acidity. In addition, the damage site to BSA molecules was analyzed by synchronous fluorescence spectra and the results exhibited that the damage site was mainly at tryptophan (Trp) residue. This study should be helpful to drive sonocatalytic method to treat tumors in clinic application.     

二次合成Y型杂原子分子筛的吸附脱硫研究

采用液-固相同晶取代反应制备骨架含Ga的Y型分子筛(AlY),研究其吸附脱除硫质量分数为500×10^-6模拟燃料中的硫化物。AlY处理含噻吩、四氢噻吩(THT)、4,6-二甲基二苯并噻吩(4,6-DMDBT)、二苯并噻吩(DBT)、苯并噻吩(BT)和4-甲基二苯并噻吩(4-MDBT)的模拟燃料时的吸附容量分别为7.0、17.4、14.5、16.9、6.9 和5.8mg(S)/g吸附剂。采用密度泛函理论(DFT)中的广义梯度近似方法(GGA)计算各分子中硫原子上的电荷数,噻吩、四氢噻吩、4,6-二甲基二苯并噻吩(4,6-DMDBT)、二苯并噻吩(DBT)、苯并噻吩(BT)和4-甲基二苯并噻吩(4-MDBT)中硫原子上电荷数分别为-0.159、-0.298、-0.214、-0.211、-0.193、-0.188。四氢噻吩和4,6-DMDBT中硫原子上的电子密度大于噻吩中硫原子上的电子密度,这就使得四氢噻吩和4,6-DMDBT中的硫原子与吸附位间的作用会明显大于噻吩中的硫原子与吸附位间的作用。采用AlY处理催化裂化汽油时的脱硫率可达68%。     

氯冉酸可见分光光度法快速测定酮康唑

建立了快速测定酮康唑含量的可见分光光度法。实验结果表明:酮康唑与氯冉酸在甲醇-丙酮混合溶剂中发生电荷转移反应,产物的最大吸收波长是524 nm,表观摩尔吸光系数为1.04×103L·mol-1·cm-1。酮康唑质量浓度在20～400 mg·L-1范围内服从比耳定律,相关系数为0.9995。当酮康唑浓度为200 mg·L-1时,五次测定结果的相对标准偏差为1.03%。测得荷移络合物的组成比和稳定常数分别为1∶1和3.9×103。回收率在98.0%以上。本方法简便、快速、准确,可作为制剂的质量控制方法。     

Design,synthesis and biological activity against estrogen receptor-dependent breast cancer of furo[1]benzofuran derivatives

The docking study on a series of furo[1]benzofuran derivatives with ERα has been demonstrated. The synthesis and characterization of a series of furo[1]benzofuran derivatives were described. All the target compounds were conducted to in vitro for the inhibitory activities against human breast cancer strains T-47D, MCF-7 and toxicity against human liver normal cell strains HL7702 via MTT assay. Most of the target compounds possessed anti-estrogen receptor-dependent breast cancer activities with weak toxicity to healthy cell strains. The preliminary structure–activity relationships were discussed.     

爆炸载荷作用下焊缝区附近埋地X70钢管的动力响应分析

爆破地震效应对埋地管线的影响已成为工程爆破领域研究的热点。基于有限元软件ANSYS/LS-DYNA,以两种含Y型焊缝(坡口有2 mm余高焊缝和坡口无余高焊缝)的埋地X70钢管为例,数值模拟研究了TNT炸药量相同(4.473 kg)而炸高(60.0、85.0和110.0 cm)不同时,焊缝区附近埋地X70钢管的动力响应规律。研究表明:当炸高为60.0 cm时,焊缝有余高的管道受应力集中的影响较大,且先于焊缝无余高的管道进入屈服阶段;当炸高为60.0和85.0 cm时,焊缝有余高的管道整体抵抗变形的能力明显弱于焊缝无余高的管道。管土间的相互作用对X70管道背爆面有支撑作用,可有效地减小管道背爆面的位移。在相同条件下,焊缝有余高的X70管道抵抗振动的性能弱于焊缝无余高的管道,且与焊缝形式相比,炸高对含焊缝区的X70管道的最大振速起主要影响作用。     

An ESR study of the oxygen transfer from excited heteroaromatic N-oxides to triethylamine to form diethyl nitroxide

The photoreaction of systems containing N-oxides and amines has been studied by ESR. The lowest triplet state of the heteroaromatic N-oxide combines with triethylamine to form an exciplex. Oxygen transfer and α-cleavage of the N-C bond in the amine part of the exciplex gave diethyl nitroxide.     

N-methyl Benzimidazole Tethered Cholic Acid Amphiphiles Can Eradicate S. aureus-Mediated Biofilms and Wound Infections

Infections associated with Gram-positive bacteria like S. aureus pose a major threat as these bacteria can develop resistance and thereby limit the applications of antibiotics. Therefore, there is a need for new antibacterials to mitigate these infections. Bacterial membranes present an attractive therapeutic target as these membranes are anionic in nature and have a low chance of developing modifications in their physicochemical features. Antimicrobial peptides (AMPs) can disrupt the microbial membranes via electrostatic interactions, but the poor stability of AMPs halts their clinical translation. Here, we present the synthesis of eight N-methyl benzimidazole substituted cholic acid amphiphiles as antibacterial agents. We screened these novel heterocyclic cholic acid amphiphiles against different pathogens. Among the series, CABI-6 outperformed the other amphiphiles in terms of bactericidal activity against S. aureus. The membrane disruptive property of CABI-6 using a fluorescence-based assay has also been investigated, and it was inferred that CABI-6 can enhance the production of reactive oxygen species. We further demonstrated that CABI-6 can clear the pre-formed biofilms and can mitigate wound infection in murine models.