Label-free detection of charged macromolecules by using a field-effect-based sensor platform: Experiments and possible mechanisms of signal generation

The possibilities and limitations of a direct electrical detection of charged macromolecules using a field-effect-based sensor platform is evaluated, mainly focusing on capacitive EIS (electrolyte-insulator-semiconductor) devices. The experimentally obtained results on the detection of DNA immobilisation and hybridisation as well as the monitoring of layer-by-layer adsorbed charged polyelectrolyte (PE) multilayers have been discussed by using two basic possible mechanisms of signal generation, namely the intrinsic charge of the macromolecules and the charge redistribution within the intermolecular spaces or in the multilayer. The effects of the layer-by-layer adsorption conditions (unbuffered and pH buffer solution), and the number and polarity of charged layers on the sensor response have been systematically investigated by means of capacitance–voltage (C–V), constant–capacitance (ConCap) and impedance spectroscopy (IS) methods. PACS 82.47.Rs; 82.80.Fk; 85.30.Tv; 87.15.Kg; 87.14.Gg     

Kegelschnitte und steinersche Ovale in Fano-Ebenen

Ohne Zusammenfassung     

1,5‐Dianilinopentane‐1,3,5‐trione: a crystal structure containing two polymorphic domains

Single crystals of the title compound, C₁₇H₁₆N₂O₃, were obtained by gas diffusion. The observed diffraction pattern is compatible with a superposition of reflections from two monoclinic unit cells with the space group C2/c. The two cells share the a and b axes but not the c axis. Both structures contain layers parallel to (001), with molecules connected by intermolecular N—H...O=C hydrogen bonds. The bonding between adjacent layers is weak. Layer displacements result in a crystal structure containing two closely related polymorphic domains. The structure of one polymorph can be derived from the structure of the other if subsequent layers are displaced by (a/4, b/4, 0) for odd‐numbered layers and by (a/4, −b/4, 0) for even‐numbered layers. Three different crystals were analysed and their observed diffraction patterns were similar, showing all three crystals to contain the polymorphic domain structure.     

柞蚕蛹皮制甲壳质

通过对柞茧蛹皮的成分、结构进行物理化学及电镜分析 ,从而确定其含有的主要成分及含甲壳质的数量 ,进而认定蛹皮的可利用价值。对提取甲壳质的工艺进行了初步研究 ,并对柞蚕蛹皮制甲壳质和虾蟹制甲壳质的性能作了比较分析     

多媒体远程教育网中基于超文本结构的数据组织

分析了现有远程教学网络中Ｗｅｂ 服务器与数据库连接方式的缺陷，在此基础上提出多媒体远程教育网络中基于超文本结构的数据组织方案，并详细设计了相应的虚拟超文本数据模型．     

纱条不匀时序模型的建立

采用Pandit Wu方法对并条纱条进行建模分析 ,提高了建模过程的程序化水平 ,可迅速建立在线预测模型。研究认为 ,并条纱条可以用AR(n)模型及ARMA(m ,n)模型进行预测控制 ,且模型的阶次一般为 2～ 5。     

煤炭的精细加工利用在西部大开发中的战略地位

提出了煤炭精细加工利用概念 ,并结合煤的结构特征 ,讨论了西部大开发中煤炭精细加工利用的新技术领域及其战略地位