Intramammary expression and therapeutic effect of a human lysozyme-expressing vector for treating bovine mastitis

To develop a gene therapy strategy for treating bovine mastitis, a new mammary-specific vector containing human lysozyme (hLYZ) cDNA and kanamycin resistance gene was constructed for intramammary expression and clinical studies. After one time acupuncture or intracisternal infusion of healthy cows with 400 μg of the p215C3LYZ vector, over 2.0 μg/ml of rhLYZ could be detected by enzymatic assay for about 3 weeks in the milk samples. Western blotting showed that rhLYZ secreted into milk samples from the vector-injected cows had molecular weight similar to that of the natural hLYZ in human colostrums. Twenty days after the primary injection, the quarters were re-injected with the same vector by quarter acupuncture and even higher concentrations of rhLYZ could be detected. Indirect competitive ELISA of milk samples showed that the vector injection did not induce detectable humoral immune response against hLYZ. Clinical studies showed that twice acupuncture of quarters with the p215C3LYZ vector had overt therapeutic effect on clinical and subclinical mastitis previously treated with antibiotics, including disappearance of clinical symptoms and relatively high microbiological cure rates. These data provide a solid rationale for using the vector to develop gene therapy for treating bovine mastitis.     

散热器型芯烘烤窑净化系统中分离器的设计研究

为解决铸铁散热器生产环境污染、产品成品率及产品质量低下等问题,笔者研制了XWJ-1型消烟雾化净化系统。该系统是根据乡镇企业实际状况,在原烘烤窑内采用烟道重新配置、油烟预处理、水雾化冷却、分离污染物、净化排放五步骤设计。通过6年多的试验运行和改进,结果证明,使用XWJ-1型油烟雾化净化系统,可以把高温、高浓度烟气中的焦油和有害气体减少90%,并分离净化到符合国家环保排放标准要求(GB9078-1996,GB16297-1996),较好地解决了热能利用、污染物回收利用等问题,提高了制芯及散热器的成品率及其质量,使企业经济效益显著提高。     

篮球运动中运动员的机能恢复方法和措施

通过对篮球运动中运动员机能恢复方法手段进行研究,论述了科学、合理的方法、手段,对加快运动员体能恢复和提高运动能力的作用;提出了教育学、医学生物学、心理学等恢复方法的种类及应用的时机.     

定床河工模型的设计制作研究

本文以南盘江防洪工程对宜良高古马段河道治理的河工模型实验为例,分析了河工模型的设计制作要求,并详细讨论了制作技巧,有效地提高了模型实验的精度。     

超高灵敏显色反应测定痕量锑──锑（Ⅲ）─罗丹明B─碘化钾体系

本文研究结果表明，水溶性大分子化合物阿拉伯胶和非离子表面活性剂ＴｒｉｔｏｎＸ－１００共同存在下，锑碘配阴离子和罗丹明Ｂ的显色反应具有很高的灵敏度。缔合物的最大吸收在５ｇ０ｎｍ，摩尔吸光系数ε＝６．１×１０￣５Ｌ·ｍｏｌ－１·ｃｍ－１。用巯基棉富集锑，分离干扰离子。本法可应用于有色金属中痕量锑的测定。     

HL－1装置中LHCD和等离子体参数的关系

本文研究了在ＨＬ－１托卡马克的不同放电阶段的低混杂波驱动特性。给出了驱动电流及驱动效率和等离子体参数，如电子平均密度ｎｅ、等离子体电流Ｉｐ及纵向磁场的关系。也给出和分析了波驱动和入射波功率的关系。在放电平段，对正反向驱动效率进行了研究和比较。     

GDX—硅胶混合薄层色谱板的效率和定量性能

对用ＧＤＸ－１０２－硅胶Ｇ、有机粘合剂研制出的混合薄层板进行性能测试，评价了其在硝基苯胺异构体、酸性食品染料分离测试方面的应用，得到好的结果。     

喷射成形过程中雾化锥体的温度场与质量场

本文着重研究了喷射成形过程中雾化锥体的温度场与质量场的测定方法，进一步测定了纯铝及ＺＱＳｎ６－６－３青铜的温度场和质量场，并讨论了其特征。     

Crystallographic report: Bis[bis(N,N‐dibenzyldithiocarbamato)cadmium(II)]

The centrosymmetric structure of {Cd[S₂CN(CH₂Ph)₂]₂}₂ features both bridging and chelating dithiocarbamate ligands so that a square pyramidal S₅ coordination geometry results. Copyright © 2004 John Wiley & Sons, Ltd.     

一类具有三角形周期环域的Hamilton系统的Poincare分支

讨论一类具有三角形周期环域的Hamilton系统在三次多项式扰动下的Poincare分支问题，证明了其Poincare分支可以产生一个极限环。．