红毛五加多糖的分离纯化和表观分子质量测定

红毛五加经热水浸提，用Sevag法脱蛋白，经DEAE—cellulose 32离子交换层析和Sephacryl S-200HR凝胶层析纯化得到3个主要多糖组分：AHP-Ⅰ、AHP-Ⅱ、AHP-Ⅲ，经HPLC和Sephacryl S-300HR凝胶层析鉴定，3种多糖均为均一组分，HPLC测定AHP-Ⅰ、AHP-Ⅱ、AHP-Ⅲ的表观分子质量分别为：7．0kD、59．5kD和12．9kD，Sephacryl S-300HR凝胶层析进行验证，得到相似结果．     

Effects of hyaluronic acid－chitosan－gelatin complex on the apoptosis and cell cycle of L929 cells

With the development in the field of tissue engineering, the interaction between biomaterials and cells has been deeply studied. Viewing the cells seeded on the surface of materials as an organic whole, cell cycle and apoptosis are analyzed to deepen the study of cell compatibility on biomaterials, while cell proliferation and differentiation are studied at the same time. In this paper, hyaluronic acid is incorporated into the chitosan-gelatin system. Propidium iodide (PI) was used in cell cycle analysis and the double-staining of cells with annexin-V and PI was applied in cell apoptosis analysis. The results show that incorporated hyaluronic acid shortens the adaptation period of cells on the material surface, and then cells enter the normal cell cycle quickly. In addition, added hyaluronic acid inhibits cell apoptosis triggered by the membranes. Therefore, hyaluronic acid improves the cell compatibility of chitosan-gelatin system and benefits the design of biomimetic materials.     

Building of hFⅨ transgenic mice by spermatogenic cells

Human FⅨ expression vector pCMVⅨ was packaged by effectene^TM reagent and injected into mice seminiferous tubules with glass pipettes.The expressional frame of pCMVⅨ was examined by PCR and Southern blotting among 41 progenies.There were 2(4%) mice being integrated with hFⅨ gene into chromosomes.4.6ng/mL of hFⅨ protein was expressed in plasma of one mouse,which was tested by ELISA.We demonstrated that building of transgenic animals by spermatogonial stem cells is an efficient method.Meanwhile,it has also been proved to be an alternative choice for mammary gland bioreactor.     

新型电液激振装置的性能研究

结合电气与液压传动的性能优势，开发出电液激振装置，推导出该装置相关参数的相互关系式。经试验得出，激振装置的振动频率、振幅、压强、流量可以根据需要进行调节。可成为煤矿、冶金、建筑等行业振动设备的一种新型激振装置。     

桩端压力注浆桩在卵石层中的应用

灌注桩采用桩端压力灌浆新技术后，能大幅度地提高基桩承裁能力，减小其沉降，在工程中已运用多年，但大量工程实践表明其对不同地层的适应性是不同的。根据某工程中钻孔灌注桩现场试验，对桩端压力灌浆技术在卵石层中灌注桩的运用效果其及经济、社会效益进行了分析。     

传统·现代性·后现代

现代性是多元、多样的,因而传统和现代性可以会通,中国的现代性也自有特色;当代西方哲学对西方现代性的批判,围绕现代性与后现代的论争,对研究与实现中国的现代性有参考价值;要加强中国现代性的哲学研究。     

滑动轴承无摩擦接触参数的边界元分析

应用边界单元法（ＢＥＭ），对平面应变情况下滑动轴承无摩擦接触时的接触参数进行了分析计算，并与常规Ｈｅｒｔｚ公式计算结果进行了对比。结果表明，用边界单元法可以提高接触参数的计算精度，并妥善解决了Ｈｅｒｔｚ公式计算值偏低的问题。另外在分析了轴承系统尺寸、材料参数及载荷变化因素而进行相应计算的基础上，给出了滑动轴承接触参数计算的经验公式。     

塑料注射模具冷却水道CAD

介绍了塑料注射模具冷却水道的计算机辅助设计。本软件为Ｐｃ级，主要应用传热学、化工原理的基本原理，开发设计了模具冷却水道计算模块，并对模具的冷却系统进行设计。     

用影响椭圆法确定建筑物保护煤柱

把引起地表点移动的地下开采范围视为椭圆形区域，提出用影响椭圆法留设建筑物保护煤柱的基本原理及其具体步骤。通过实例表明，该法适应于各类受护边界形状及基岩和煤层标高变化较大情况下的保护煤柱留设，是确定矿区建筑物保护煤柱的新方法。     

红外测温仪在纺织上使用的条件及方法

介绍了红外测温仪在纺织测温方面的应用情况．就预置试样的发射率不同、测量位置不同及试样受到红外辐射的照度不同等３种情况对红外测温仪测量结果的影响，探讨了准确测温的最佳使用条件和方法．