An effective column‐switching counter‐current chromatography (CCC) protocol combining stepwise elution mode was successfully developed for simultaneous and preparative separation of anti‐oxidative components from ethyl acetate extract of traditional Chinese herbal medicine Rubia cordifolia. The column‐switching CCC system was interfaced by a commercial low‐pressure six‐port switching valve equipped with a sample loop, allowing large volume introduction from the first dimension (1st‐D) to the second dimension (2nd‐D). Moreover, to extend the polarity window, three biphasic liquid systems composed of n‐hexane/ethyl acetate/methanol/water (1:2:1:2, 2:3:2:3, 5:6:5:6 v/v) were employed using stepwise elution mode in the 1st‐D. By valve switching technique the whole interested region of 1st‐D could be introduced to second dimension for further separation with the solvent system 5:5:4:6 v/v. Using the present column‐switching CCC protocol, 500 mg of crude R. cordifolia extract were separated, producing milligram‐amounts of four anti‐oxidative components over 90% pure. Structures of purified compounds were identified by 1H and 13C NMR. 相似文献
In this paper, we study the random dynamical system generated by a stochastic reaction‐diffusion equation with multiplicative noise and prove the existence of an ‐random attractor for such a random dynamical system. The nonlinearity f is supposed to satisfy some growth of arbitrary order . 相似文献
A fluorescent probe for the sensitive and selective determination of sulfide ions is presented. It is based on the use of graphene quantum dots (GQDs) which emit strong and stable blue fluorescence even at high ionic strength. Copper(II) ions cause aggregation of the GQDs and thereby quench fluorescence. The GQDs-Cu(II) aggregates can be dissociated by adding sulfide ions, and this results in fluorescence turn on. The change of fluorescence intensity is proportional to the concentration of sulfide ions. Under optimal conditions, the increase in fluorescence intensity on addition of sulfide ions is linearly related (r2 = 0.9943) to the concentration of sulfide ions in the range from 0.20 to 20 μM, and the limit of detection is 0.10 μM (at 3 σ/s). The fluorescent probe is highly selective for sulfide ions over some potentially interfering ions. The method was successfully applied to the determination of sulfide ions in real water samples and gave recoveries between 103.0 and 113.0 %.
Graphene quantum dots (GQDs) emit strong blue fluorescence which, however, is quenched by copper(II) ions due to the formation of GQDs-Cu(II) aggregates. Fluorescence is recovered by sulfide ions due to the dissociation of GQDs-Cu(II) aggregates.
A C2‐symmetric Schiff‐base ligand, derived from tridentate‐Schiff‐base, was developed and successfully applied to the asymmetric Michael addition of nitroalkanes to 2‐enoyl‐pyridine N‐oxides. With this newly catalytic system, an unprecedented diastereoselectivity was obtained in the asymmetric Michael addition of nitroalkanes to 2‐enoyl‐pyridine N‐oxides. In addition, a switch in enantioselectivity was achieved by using this newly catalytic system and our previous catalyst. After a facile reduction, the optically active adduct was converted to a biologically active dihydro‐2H‐pyrrol 4 a . Furthermore, a connection of two tridentate‐Schiff‐base subunits proved to be an effective strategy in ligand design. 相似文献
The forced convection heat transfer with water vapor condensation is studied both theoretically and experimentally when wet
flue gas passes downwards through a bank of horizontal tubes. Extraordinarily, discussions are concentrated on the effect
of water vapor condensation on forced convection heat transfer. In the experiments, the air–steam mixture is used to simulate
the flue gas of a natural gas fired boiler, and the vapor mass fraction ranges from 3.2 to 12.8%. By theoretical analysis,
a new dimensionless number defined as augmentation factor is derived to account for the effect of condensation of relatively
small amount of water vapor on convection heat transfer, and a consequent correlation is proposed based on the experimental
data to describe the combined convection–condensation heat transfer. Good agreement can be found between the values of the
Nusselt number obtained from the experiments and calculated by the correlation. The maximum deviation is within ±6%. The experimental
results also shows that the convection–condensation heat transfer coefficient increases with Reynolds number and bulk vapor
mass fraction, and is 1∼3.5 times that of the forced convection without condensation. 相似文献