Multiplasmid DNA in agarose gel electrophoresis: Suitable dye and temperature is essential for prominent profiles

Nucleic acids dye Goldview is widely used in agarose gel electrophoresis (AGE). However, in this study, a sample of multiplasmid DNA (multi-pDNA) stained with Goldview analyzed by AGE showed its instability at low temperature. Three types of DNA samples were analyzed, including linear DNA (ladder), single-plasmid DNA (single-pDNA), and multi-pDNA, electrophoretic conditions were optimized by adjusting the dye, the buffer, and the temperature (1–50°C). The results showed that the light intensity of Gelred is 2.2-times higher than that of Goldview in staining multi-pDNA. Compared with the single-pDNA and the linear DNA, the multi-pDNA stained with Goldview was greatly affected by temperature. This short communication indicated that Gelred is a highly applicable dye for analyzing multiplasmid samples. The degree and the way of binding of Goldview to multi-pDNA are greatly affected by temperature.     

Ultra-high Tg and ultra-low coefficient of thermal expansion polyimide films based on hydrogen bond interaction

To tolerate high processing temperature during the fabrication of low-temperature polycrystalline silicon thin-film transistors (LTPS–TFT) in flexible OLED devices, the polyimide (PI) films, which are used as substrate, should have ultra-high glass transition temperature (T_g > 450°C) and ultra-low coefficient of thermal expansion (CTE at 0–5 ppm K⁻¹). In this paper, two novel heterocyclic monomers, namely, N,N'-(xanthone-2,7-diyl)bis(4-aminobenzamide) (p-DAXBA) and N,N'-(xanthone-2,7-diyl)bis(3-aminobenzamide) (m-DAXBA), which contain a xanthone moiety, are prepared and polycondensed with pyromellitic dianhydride (PMDA), respectively. PI films (PIa and PIb) with intrinsic high T_g and low CTE are designed from the perspective of rigid conjugate xanthone structure and hydrogen bonding interaction. It is found that the PIa films prepared by p-DAXBA have better linear structure of molecular chains and show relatively higher T_g and lower CTE. The T_g of PIa-40 is greater than 450°C, and CTE can reach as low as 2.7 ppm K⁻¹, tensile strength of 179 MPa, modulus of 5.67 GPa, indicating potential application prospect as a flexible OLED substrate.     

美国极轨气象卫星的转型探索

气象卫星是重要的国家基础性、战略性空间系统.美国极轨气象卫星体系通过融合美国国防部、美国海洋和大气管理局及欧洲气象卫星组织各自的极轨气象卫星系统,实现了全球全天时的持续覆盖能力.为获取高时效性全球范围气象卫星资料,近年来美国一直在探索气象卫星的转型发展.在梳理美国极轨气象卫星发展历程的基础上,分析了其多种转型探索,包括对美国国家海洋和大气管理局开展的新一代卫星架构研究项目,试点采购商业气象卫星数据,开展气象小卫星关键技术及载荷研究等.     

Selective annulation of benzamides with internal alkynes catalyzed by an electron-deficient rhodium catalyst

An electron-deficient [Cp^ERhCl₂]₂ catalyzed annulation of N-pentafluorophenylbenzamides with internal alkynes was successfully established under mild reaction conditions, with the assistance of Lewis acid silver salt. Particularly, electron-deficient benzamide substrates were smoothly transformed into the desired products in this catalytic system. The catalytic system showed a broad tolerance for different substituents on the aromatic rings or aryl, alkyl-substituted alkynes.     

由正负磁单极对相互作用诱导的孤立狄拉克弦

基于三维旋量Gross-Pitaevskii(GP)方程研究在含时周期性外磁场作用下玻色-爱因斯坦凝聚体的动力学行为.结果显示,在含时周期外磁场的作用下,铁磁态自旋为1的玻色-爱因斯坦凝聚体将发生拓扑形变.当磁场的两个零点进入凝聚体后,自旋向上态的密度布居图在z轴上分别形成向上和向下的凸起.随着磁场的两个零点在凝聚体内...     

Nonlinear spectroscopy of three-photon excitation of cesium Rydberg atoms in vapor cell

We present nonlinear spectra of four-level ladder cesium atoms employing 6 S1/2→6 P3/2→7 S1/2→30 P3/2 scheme of a room temperature vapor cell.A coupling laser drives Rydberg transition,a dressing laser couples two intermediate levels,and a probe laser optically probes the nonlinear spectra via electromagnetically induced transparency(EIT).Nonlinear spectra are detected as a function of coupling laser frequency.The observed spectra exhibit an enhanced absorption(EA) signal at coupling laser resonance to Rydberg transition and enhanced transmission(ET) signals at detunings to the transition.We define the enhanced absorption(transmission) strength,HEA(HET),and distance between two ET peaks,γET,to describe the spectral feature of the four-level atoms.The enhanced absorption signal HEA is found to have a maximum value when we vary the dressing laser Rabi frequency Ωd,corresponding Rabi frequency is defined as a separatrix point,ΩdSe.The values of ΩdSe and further η=ΩdSe/Ωc are found to depend on the probe and coupling Rabi frequency but not the atomic density.Based on ΩdSe,the spectra can be separated into two regimes,weak and strong dressing ranges,Ωd≤ΩdSe and Ωd≥QdSe,respectively.The spectroscopies display different features at these two regimes.A four-level theoretical model is developed that agrees well with the experimental results in terms of the probe-beam absorption behavior of Rabi frequency-dependent dressed states.     

Unveiling the Layer‐Dependent Catalytic Activity of PtSe2 Atomic Crystals for the Hydrogen Evolution Reaction

Two‐dimensional (2D) PtSe₂ shows the most prominent layer‐dependent electrical properties among various 2D materials and high catalytic activity for hydrogen evolution reaction (HER), and therefore, it is an ideal material for exploring the structure–activity correlations in 2D systems. Here, starting with the synthesis of single‐crystalline 2D PtSe₂ with a controlled number of layers and probing the HER catalytic activity of individual flakes in micro electrochemical cells, we investigated the layer‐dependent HER catalytic activity of 2D PtSe₂ from both theoretical and experimental perspectives. We clearly demonstrated how the number of layers affects the number of active sites, the electronic structures, and electrical properties of 2D PtSe₂ flakes and thus alters their catalytic performance for HER. Our results also highlight the importance of efficient electron transfer in achieving optimum activity for ultrathin electrocatalysts. Our studies greatly enrich our understanding of the structure–activity correlations for 2D catalysts and provide new insight for the design and synthesis of ultrathin catalysts with high activity.     

Antidark solitons and soliton molecules in a (3 + 1)-dimensional nonlinear evolution equation

Nonlinear Dynamics - We investigate a (3 + 1)-dimensional nonlinear evolution equation which is a higher-dimensional generalization of the Korteweg–de Vries equation. On the...