Near-infrared Raman spectroscopy of single optically trapped biological cells

We report on the development and testing of a compact laser tweezers Raman spectroscopy (LTRS) system. The system combines optical trapping and near-infrared Raman spectroscopy for manipulation and identification of single biological cells in solution. A low-power diode laser at 785 nm was used for both trapping and excitation for Raman spectroscopy of the suspended microscopic particles. The design of the LTRS system provides high sensitivity and permits real-time spectroscopic measurements of the biological sample. The system was calibrated by use of polystyrene microbeads and tested on living blood cells and on both living and dead yeast cells. As expected, different images and Raman spectra were observed for the different cells. The LTRS system may provide a valuable tool for the study of fundamental cellular processes and the diagnosis of cellular disorders.     

矩阵方程A^HXA=B的反自反解及其最佳逼近

利用广义奇异值分解定理，得到了矩阵方程A^HXA=B的反自反解存在的一个充要条件，并获得了相应的通解表达式和最佳逼近解，最后获得了最小范数解。     

矩阵方程AHXA=B的反自反解及其最佳逼近

利用广义奇异值分解定理,得到了矩阵方程AHXA=B的反自反解存在的一个充要条件,并获得了相应的通解表达式和最佳逼近解,最后获得了最小范数解     

Inhibition of Alkaline Phosphatase from Pearl Oyster Pinctada fucata by o-Phthalaldehyde： Involvement of Lysine and Histidine Residues at the Active Site

Alkaline phosphatase from Pinctada fucata was inactivated by o-phthalaldehyde （OPA）. The inactivation followed pseudo first-order kinetics with a second rate constant of 0.167 （mmol/L）^-1·min^-1 at pH 7.5 and 25℃. A Tsou＇s plot analysis showed that inactivation occurred upon formation of one isoindole group. The OPA-modified enzyme lost the ability to bind with the specific affinity column and the presence of substrates or competitive inhibitors protected the enzyme from inactivation. The results revealed that the OPA-reaction site was at the enzyme substrate binding site. Prior modification of the enzyme by lysine or histidine specific reagent abolished formation of the isoindole derivatives, suggesting that lysine and histidine residues were involved in the OPA-induced inactivation. Taken together, OPA inactivated the alkaline phosphatase from Pinctada fucata by cross-linking lysine and histidine residues at the active site and formed an isoindole group at the substrate binding site of the enzyme.     

Study on molecular interaction between NifA and NifL of Pseudomonas stutzeri A1501 by using the yeast two-hybrid system

Pseudomonas stutzeri AI501 (formerly Alcaligenes faecalis A1501), which was isolated from rice paddies in South China in 1980, can colonize tightly on rhizoplane of the host plants or invade the roots of plants for growth and nitrogen fixation. But A1501 can fix nitrogen only under the micro-aerobic and nitrogen-free conditions. The oxygen concentration and the availability of fixed nitrogen are therefore important factors in the regulation of nitrogenase biosynthesis of associative nitrogen-fixing bacteria.     

四重马氏平稳过程的非线性预测问题

假设{X（t），t∈R^1}是由广义Wiener随机积分所定义的四重马氏平稳过程．如果该随机过程{X（t），t∈R^1}被一有界Borel可测函数f（＊）变换，则得到新的随机过程，记为Y（t）=f（X（t））．作者在本文中，首先粗略地研讨了四重马氏平稳过程{X（t），t∈R^1}及其均方导数的一些概率性质．其次，对于一些构造较简单的Borel可测函数f（＊），较详细地探讨了随机过程{Y（t）=f（X（t））}的非线性均方预测问题．     

个人所得税收入分配效应的模型分析

通过分析征税行为对征税前后的基尼系数的影响,讨论了个人所得税的收入分配效应.结论是只有当税赋基尼系数大于税前基尼系数时,征收个人所得税才能对收入分配起到调节作用.     

Materials informatics and study on its further development

NOWADAYS,WITH THE DEVELOPMENT OF MATERIALS SCI-ENCE AND TECHNOLOGY AND WITH THE CONTINUOUS EMER-GENCE OF NEW MATERIALS,TECHNOLOGIES,PROCESSES AND METHODS,PROLIFERATION AND COMPLEXITY OF MATERIAL SCI-ENCE DATA HAVE INCREASED ENORMOUSLY.THEREFORE,DIFFI-CULT…     

Experimental study to confirm the existence of hydrocarbon under high pressure and temperature of deep lithosphere

Previous studies indicated that the existence of over- pressure in sedimentary basins not only affects the as- sessment of oil and gas resources, but also closely re- lates to the accumulation and preservation of petroleumin deep reservoirs[1,2]. It is well known that there are more than 180 overpressure basins in the world. Be- cause fluid pressure is transient in sedimentary basin over geologic time, the effect of increasing pressure on organic matter metamorphism is difficult to determine, …     

电大尺寸载体天线间宽带干扰耦合快速分析

为求解电大尺寸载体天线间的宽带干扰耦合度,建立了天线间干扰耦合分析模型,提出了自适应多层快速多极子算法作为求解该耦合分析模型的核心算法.该算法将阻抗积分表达式中的基函数和权函数分别用不同空间位置上的Dirac函数展开,使阻抗积分的计算得到大大简化,所有转移过程可由快速傅里叶变换计算完成,应用波形渐进估计技术计算载体表面及天线上的宽带电流值,最后结合微波二端口网络理论求得天线间的宽带耦合度.数值实验和实测结果证明,在精度相等的前提下,所提出的快速分析方法比传统快速分析方法的计算效率和存储效率均提高了30%左右.