差压式液体流量计的设计

针对现有各流量计机械结构较复杂、低流与微流难以检测的不足,设计了一种基于差压原理的一种新型液体流量计．本流量计以特制的导管和差压传感器作为流量检测元件,用STCl2C5410AD作为微处理器．检测元件将差压信号转换为电信号,该电信号经仪表放大器放大后,用微处理器进行数据采集和处理,获得流量和流速值．     

Andrographolide Inhibits Biofilm and Virulence in Listeria monocytogenes as a Quorum-Sensing Inhibitor

Listeria monocytogenes is a major foodborne pathogen that can cause listeriosis in humans and animals. Andrographolide is known as a natural antibiotic and exhibits good antibacterial activity. We aimed to investigate the effect of andrographolide on two quorum-sensing (QS) systems, LuxS/AI-2 and Agr/AIP of L. monocytogenes, as well as QS-controlled phenotypes in this study. Our results showed that neither luxS expression nor AI-2 production was affected by andrographolide. Nevertheless, andrographolide significantly reduced the expression levels of the agr genes and the activity of the agr promoter P₂. Results from the crystal violet staining method, confocal laser scanning microscopy (CLSM), and field emission scanning electron microscopy (FE-SEM) demonstrated that andrographolide remarkably inhibited the biofilm-forming ability of L. monocytogenes 10403S. The preformed biofilms were eradicated when exposed to andrographolide, and reduced surviving cells were also observed in treated biofilms. L. monocytogenes treated with andrographolide exhibited decreased ability to secrete LLO and adhere to and invade Caco-2 cells. Therefore, andrographolide is a potential QS inhibitor by targeting the Agr QS system to reduce biofilm formation and virulence of L. monocytogenes.     

大林算法在位置随动系统中的应用

为了解决含有纯滞后惯性环节的位置随动系统采用常规的直接数字控制方法较复杂且效果不佳的问题,提出利用大林算法来处理其纯滞后的惯性环节。通过对大林控制器的详细设计过程和对位置随动系统的实例仿真可以看出,大林控制器具有良好的鲁棒性和较高的灵敏度,最后得出了大林算法在含有纯滞后惯性环节的位置随动系统中具有应用价值的结论。     

基于对称相关函数法的线谱检测

在被动声纳系统中,线谱检测是一个至关重要的问题。文中利用对称相关函数理论,分析了抵消平稳随机过程中与信号不相关的加性随机噪声的机理,并应用于线谱信号检测。仿真结果表明,该方法比传统的相关法有效,对线谱检测,优于经典功率谱检测方法。     

一种基于卷积神经网络的区域调光技术

由于光线串扰,像素补偿算法难以根据提取出的背光信息进行准确补偿,同时,单一补偿曲线难以适应具有不同亮度特点的图像内容,导致补偿图像的平均质量不高.为了提高像素补偿算法对复杂图像内容的适应性,本文引入神经网络中的编码和解码思想,通过编码网络提取图像深层特征,在解码网络中利用浅层特征的信息对深层特征进行解码,提出了一种联合...     

利用FPGA实现图像鉴别数码相机硬件设计与探讨

本文讨论了FPGA逻辑实现原理及其实现方式,FPGA应用于数码相机的图像鉴别,通过静止图像内容的任何改变,都将反映在嵌入图像中的数字水印上的方式来鉴别图像快照.提出了一种利用FPGA实现图像鉴别可靠数码相机硬件设计方案.     

The Antibacterial Activity Mode of Action of Plantaricin YKX against Staphylococcus aureus

We aimed to evaluate the inhibitory effect and mechanism of plantaricin YKX on S. aureus. The mode of action of plantaricin YKX against the cells of S. aureus indicated that plantaricin YKX was able to cause the leakage of cellular content and damage the structure of the cell membranes. Additionally, plantaricin YKX was also able to inhibit the formation of S. aureus biofilms. As the concentration of plantaricin YKX reached 3/4 MIC, the percentage of biofilm formation inhibition was over 50%. Fluorescent dye labeling combined with fluorescence microscopy confirmed the results. Finally, the effect of plantaricin YKX on the AI-2/LuxS QS system was investigated. Molecular docking predicted that the binding energy of AI-2 and plantaricin YKX was −4.7 kcal/mol and the binding energy of bacteriocin and luxS protein was −183.701 kcal/mol. The expression of the luxS gene increased significantly after being cocultured with plantaricin YKX, suggesting that plantaricin YKX can affect the QS system of S. aureus.     

组织工程多孔管状支架的低温挤出成形

聚乙二醇/聚对苯二甲酸丁二醇酯(PEG/PBT)-1,4二氧六环溶液体系在温度低于20℃时可变成凝胶态。将数字挤压/喷射技术与热致相分离技术结合,提出了一种新型多孔管状支架成形技术——低温挤出成形技术。构建了基于该技术的成形平台,研究了成形温度对支架宏观形貌、孔隙率、力学性能等的影响。该技术成形的支架孔隙率最高可达95%,微孔尺寸分布为35~48μm,支架弹性模量为61.9~106 kPa。人类颌下腺细胞种植实验表明:支架具有良好的细胞亲和性,在涎腺组织工程中具有良好的应用前景。     

显色基质法定量测定质粒DNA中的细菌内毒素

探讨使用显色基质法定量测定质粒DNA中细菌内毒素含量的可行性。结果表明,质粒DNA溶液经2倍稀释,对内毒素检测不存在干扰作用,用显色基质法可以定量测定质粒DNA中内毒素含量。显色基质法与家兔热源法比较,有敏捷、快速、定量、稳定可行的优点,其测定结果同家兔热源法结果一致。