Fluorescent Aptamer-Polyethylene Glycol Functionalized Graphene Oxide Biosensor for Profenofos Detection in Food

A biosensor based on self-assembled ssDNA(aptamer) and polyethylene glycol functionalized graphene oxide(GO-PEG) has been designed for sensing profenofos in food. The sensor has employed the fluorescence on/off switching strategy in a single step in homogeneous solution. Compared to traditional detection methods, the strategy proposed here is simple, convenient, fast and sensitive. Furthermore, compared with the general aptamer-GO structure, this aptamer-GO-PEG structure is in possession of a better detection performance, which is largely attributed to the improvement of the biocompatibility and the adjustment of the adsorption capacity of GO by grafting the blocking agent PEG onto the surface of GO. Additionally, the improved biocompatibility of GO shows better stability in salt solutions and physiological solutions, which is more conducive to its practical application in foods. In this project, profenofos had been detected with the proposed strategy, and the limit of detection has been controlled to be 0.21 ng/mL. This aptasensing assay has been applied to determining profenofos in (spiked)tap water, cabbage and milk with the recovery values ranging from 93.1% to 108.5%, from 90.8% to 113.2% and from 105.9% to 114.2%, respectively.     

直驱式电液伺服阀用线性力马达耐高温优化设计

线性力马达研究在高温下永磁铁存在退磁、线圈散热能力下降导致许用电流降低等问题,对材料选择和结构优化设计提出了挑战.文中从线圈参数、气隙结构、永磁材料等方面对6通径DDV阀用线性力马达进行优化设计.利用Ansoft Maxwell软件建立线性力马达有限元仿真模型,针对线圈结构参数,重点研究安匝数的优化选择;针对气隙结构参数,研究气隙宽度、高度等因素对马达力性能的影响;对耐高温永磁体的材料进行优化选择.仿真与实验结果表明:优化后的线性力马达耐高温能力和输出力显著提高,工作油温最高可达200℃,驱动力在100 N以上.     

外加压力时双纳米线中浅施主杂质的性质

在有效质量近似下，采用简单的尝试波函数变分地计算了压力对浅施主杂质在对称GaAs／Ga1-xAlxAs双纳米线中的束缚能的影响：计算了线宽、施主离子位置及垒宽对体系束缚能的影响，发现随压力增加和垒宽的减小双线间的耦合越来越强；体系束缚能随线宽显示出非线性行为，并且在线宽较小时体系束缚能有一最大值，所得结果和前人计算符合很好。     

菌丝体的膜流动性对去饱和酶活性的影响

以DPH和ANS标记深黄被孢霉的生物膜，考察了生物膜流动性的变化与去饱和酶活性的关系，结果表明，生物膜流动性下降，活化了△12和△6去饱和酶，进行合成了大量的亚油酸和亚麻酸；生物膜脂中脂肪酸不饱和度的提高可使生物膜的流动性提高，这可能是在低温下，微生物保持正常的物质和能量代谢的自我调控，从而为高不饱和脂肪酸的生物合成调控提供了有用的信息。     

蓝牙技术在工业系统中的应用

就蓝牙技术如何能用于不同工业现场设备间的连接进行了讨论.把蓝牙技术在工业现场的应用分成三类:替代串行电缆;与网络技术的融合;连接有线网络.同时讨论了蓝牙技术运用于工业环境的可行性与局限性,以及工业环境下对蓝牙技术的要求.