Use of zwitterionic buffer additives to improve the separation of proteins in capillary zone electrophoresis

In CZE, the adsorption of the proteins on the capillary wall is a common problem. This paper describes the simple method of utilizing zwitterionic buffer additives to improve the separation of proteins in untreated fused silica capillaries at neutral pH. Three kinds of zwitterion are evaluated in the separation of acidic, neutral, and basic proteins, including their effect on protein efficiency, mobility, separation, and resolution; the difference between the effects of the different additives are also highlighted. The method has proved to be a possible means of reducing protein adsorption, especially for basic proteins.     

Synthesis and characterization of novel thermoresponsive‐co‐biodegradable hydrogels composed of N‐isopropylacrylamide,poly(L‐lactic acid), and dextran

A series of novel multifunctional hydrogels that combined the merits of both thermoresponsive and biodegradable polymeric materials were designed, synthesized, and characterized. The hydrogels were copolymeric networks composed of N‐isopropylacrylamide (NIPAAM) as a thermoresponsive component, poly(L‐lactic acid) (PLLA) as a hydrolytically degradable and hydrophobic component, and dextran as an enzymatically degradable and hydrophilic component. The chemical structures of the hydrogels were characterized by an attenuated total reflection–Fourier transform infrared spectroscopy (ATR–FTIR) technique. The hydrogels were thermoresponsive, showing a lower critical solution temperature (LCST) at approximately 32 °C, and their swelling properties strongly depended on temperature changes, the balance of the hydrophilic/hydrophobic components, and the degradation of the PLLA component. The degradation of the hydrogels caused by hydrolytic cleavage of ester bonds in the PLLA component was faster at 25 °C below the LCST than at 37 °C above the LCST, determined by the ATR–FTIR technique. Due to their multifunctional properties, the designed hydrogels show great potential for biomedical applications, including drug delivery and tissue engineering. © 2004 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 42: 5054–5066, 2004     

浅谈普通话的可感性特点

作为审美对象,普通话和其它美的形象一样,是具有特殊规定性的形式和内容的统一体,从形式上看,普通话的可感形象体现在它的语音美及节奏美。     

HPA/MXOY杂多酸在合成异戊酯上的应用研究

本文研究了HPA/MXOY固体杂多酸催化剂的制备及负载,并以异戊醇合成异戊酯为探针,用硅盐、锆盐、活性炭等物质为载体,进行了催化效果的研究及表征。实验结果得出:HPA/ZrO型杂多酸催化剂效果最佳,酯收率可达82%。     

关于计量经济学学习的几点思考

计量经济学是一门独特的学科,它的学科基础十分广泛,学习的方法与思维有别于其他的学科,并且实际的操作性很强,从宏观的角度把握了它的一些特点,有利于从总体上理解并学好计量经济学。     

PRELIMINARY STUDY ON RETRO-REFLECTIVE COATED PAPER BASED ON MICROGLASS BEADS

INTRODUCTIONThe micro-glass bead[1-2] is a new kind of silicate material, with good properties of chemical stability, mechanical strength as well as electro-resistance. When its diameter is below 0.08mm and the reflective index is in the range of 1.9~2.1, retro-reflective characteristics can be achieved. When a beam of ray from any direction irradiates the surface of the micro-glass beads, it can be converged at the special reflective layer consisted of focuses of micro-glass beads, owing …     

The best uniform convergence rate of linear empirical bayes estimators

In this paper the thought on the uniform convergence of an empirical Bayes estimator or linear empirical Bayes (l.e.B.) estimator is advanced. Under two different models the l.e.B. estimators of the parameter are constructed respectively. It is proved that the convergence rates and uniform convergence rates of these l.e.B. estimators are all one with respect to the corresponding prior families. It is shown that the uniform convergence rate one is the best, under mild assumptions imposed on the conditional density of the sample.     

算子子空间的D_σ~(n)(r)性质的一个充分条件

给出了一个确定算子子空间具有性质D_σ~(n)(r)的方法。     

一类特殊的奇异级数

讨论了华林问题中一类特殊的奇异级数，在分析的范围内用代数数论的方法对它的下界作出一个估计，从而改进了Vaughan的结果。