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51.
A new synthesis of N1-(substituted)-pyrimido[5,4-e]-1,2,4-triazine-5,7(1H,6H)-diones, which are analogues of the natural product toxoflavin, is reported. Condensation of preformed alkyl or aryl hydrazones with 6-chloro-3-methyl-5-nitrouracil efficiently provides pyrimidotriazinediones in a three-step process that broadens the scope of R1 substituents. 相似文献
52.
Bernie OHare Alan J. Benesi Scott A. Showalter 《Journal of magnetic resonance (San Diego, Calif. : 1997)》2009,200(2):354-358
Exclusively heteronuclear 13C-detected NMR spectroscopy of proteins in solution has seen resurgence in the past several years. For disordered or unfolded proteins, which tend to have poor 1H-amide chemical shift dispersion, these experiments offer enhanced resolution and the possibility of complete heteronuclear resonance assignment at the cost of leaving the 1H resonances unassigned. Here we report two novel 13C-detected NMR experiments which incorporate a 1H chemical shift evolution period followed by 13C-TOCSY mixing for aliphatic 1H resonance assignment without reliance on 1H detection. 相似文献
53.
为确定拟南芥中一个经典阿拉伯半乳糖蛋白(arabinogalactan-protein,AGP)的亚细胞定位,克隆了这个基因的编码区,与绿色荧光蛋白(GFP)基因构建融合表达载体,经农杆菌介导转化烟草BY-2悬浮细胞.利用激光扫描共聚焦显微镜,在稳定转化的BY-2细胞表面观察到绿色荧光.研究结果表明,此AGP分布在细胞膜表面. 相似文献
54.
Spatiotemporal self-organization is found in a wide range of distributed dynamical systems. The coupling of the active elements in these systems may be local or global or within a network, and the interactions may be diffusive or nondiffusive in nature. The articles in this focus issue describe biological and chemical systems designed to exhibit spatiotemporal dynamics and the control of such dynamics through feedback methods. 相似文献
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The mathematical formulation and analysis of the Barenblatt–Biot model of elastic deformation and laminar flow in a heterogeneous porous medium is discussed. This describes consolidation processes in a fluid‐saturated double‐diffusion model of fractured rock. The model includes various degenerate cases, such as incompressible constituents or totally fissured components, and it is extended to include boundary conditions arising from partially exposed pores. The quasi‐static initial–boundary problem is shown to have a unique weak solution, and this solution is strong when the data are smoother. Copyright © 2002 John Wiley & Sons, Ltd. 相似文献
57.
G. F. Carey B. N. Jiang R. Showalter 《Numerical Methods for Partial Differential Equations》1988,4(3):165-171
A regularization procedure for nonlinear conservation equations is introduced and demonstrated to have a stabilizing effect on the numerical solution of the associated approximate problem. Representative results for a least-squares finite-element method are given, and the numerical performance of the stabilization procedure explored. The effect of the regularization term is similar to a local numerical dissipation dependent on the numerical itegration time step. 相似文献
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Mihaliuk E Sakurai T Chirila F Showalter K 《Faraday discussions》2001,(120):383-94; discussion 407-19