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71.
Emi Hifumi Naruhiko Kubota Yoshiyuki Niimi Kosuke Shimizu Naoyoshi Egashira Taizo Uda 《Analytical sciences》2002,18(8):863-867
Highly sensitive detection of proteins in serum becomes difficult in some cases during surface plasmon resonance (SPR) measurements, because some ingredients in the serum hugely enhance non-specific reactions on the sensing chip of SPR. It is well recognized that the antibody against core protein p24 of HIV in serum is one of the most important proteins in the accurate diagnosis of infection with HIV. In this study, we could attain the accurate detection of anti p24 antibody in human serum by eliminating the serious effects of the ingredients in serum on the measurement of SPR by employing these procedures: 1) blocking the gold surface of the sensing chip with human serum and 2) heating the serum sample at 56 degrees C for 30 min. Without these treatments, the signal of SPR was considerably suppressed on the measurements of the anti p24 antibody which contained human serum, making the accurate detection difficult. However, with introducing the above two treatments, the sensing of anti p24 antibody in human serum was improved, while a small non-specific reaction was still observed. By removing the non-specific reaction caused by the ingredients in the serum, we could accurately measure the antibody for p24 in human serum sample over the range from 1 to 20 micrograms/ml. 相似文献
72.
73.
The letter describes a novel concept for the synthesis of biologically important arylphenylmethanols. Stereoselective cyclohexadienyl transfer from 1,4-cyclohexadienyltributyltin to various aromatic aldehydes using AgOTf/BINAP as a catalyst precursor provides 1,4-cyclohexadienylphen-ylmethanols that are readily oxidized to the corresponding arylphenylmethanols. Fifteen examples are presented. 相似文献
74.
Saadat I Higashi H Obuse C Umeda M Murata-Kamiya N Saito Y Lu H Ohnishi N Azuma T Suzuki A Ohno S Hatakeyama M 《Nature》2007,447(7142):330-333
Helicobacter pylori cagA-positive strains are associated with gastritis, ulcerations and gastric adenocarcinoma. CagA is delivered into gastric epithelial cells and, on tyrosine phosphorylation, specifically binds and activates the SHP2 oncoprotein, thereby inducing the formation of an elongated cell shape known as the 'hummingbird' phenotype. In polarized epithelial cells, CagA also disrupts the tight junction and causes loss of apical-basolateral polarity. We show here that H. pylori CagA specifically interacts with PAR1/MARK kinase, which has an essential role in epithelial cell polarity. Association of CagA inhibits PAR1 kinase activity and prevents atypical protein kinase C (aPKC)-mediated PAR1 phosphorylation, which dissociates PAR1 from the membrane, collectively causing junctional and polarity defects. Because of the multimeric nature of PAR1 (ref. 14), PAR1 also promotes CagA multimerization, which stabilizes the CagA-SHP2 interaction. Furthermore, induction of the hummingbird phenotype by CagA-activated SHP2 requires simultaneous inhibition of PAR1 kinase activity by CagA. Thus, the CagA-PAR1 interaction not only elicits the junctional and polarity defects but also promotes the morphogenetic activity of CagA. Our findings revealed that PAR1 is a key target of H. pylori CagA in the disorganization of gastric epithelial architecture underlying mucosal damage, inflammation and carcinogenesis. 相似文献
75.
Dr. Samir Das Dr. Arundhati Nag JingXin Liang Dr. David N. Bunck Dr. Aiko Umeda Blake Farrow Matthew B. Coppock Deborah A. Sarkes Amethist S. Finch Heather D. Agnew Suresh Pitram Bert Lai Mary Beth Yu Dr. A. Katrine Museth Dr. Kaycie M. Deyle Bianca Lepe Frances P. Rodriguez‐Rivera Amy McCarthy Belen Alvarez‐Villalonga Ann Chen John Heath Dimitra N. Stratis‐Cullum Prof. James R. Heath 《Angewandte Chemie (International ed. in English)》2015,54(45):13219-13224
We describe a general synthetic strategy for developing high‐affinity peptide binders against specific epitopes of challenging protein biomarkers. The epitope of interest is synthesized as a polypeptide, with a detection biotin tag and a strategically placed azide (or alkyne) presenting amino acid. This synthetic epitope (SynEp) is incubated with a library of complementary alkyne or azide presenting peptides. Library elements that bind the SynEp in the correct orientation undergo the Huisgen cycloaddition, and are covalently linked to the SynEp. Hit peptides are tested against the full‐length protein to identify the best binder. We describe development of epitope‐targeted linear or macrocycle peptide ligands against 12 different diagnostic or therapeutic analytes. The general epitope targeting capability for these low molecular weight synthetic ligands enables a range of therapeutic and diagnostic applications, similar to those of monoclonal antibodies. 相似文献
76.
Ebisu T Katsuta K Fujikawa A Aoki I Umeda M Naruse S Tanaka C 《Magnetic resonance imaging》2001,19(2):153-160
The immunosuppressive drug FK506 (tacrolimus) has been reported to be a powerful neuroprotective agent in the focal ischemia of animals. However, no report has been published concerning neuroprotective effect of this compound on the morphology in superacute stage. The separate analysis between early and delayed effects of FK506 on the morphology may be helpful in the study of the compound's mechanism of action which is still unknown. The goal of this study was to determine early and delayed effects of pharmacological treatment with FK506 in permanent MCA occlusion using magnetic resonance imaging (MRI). Nineteen rats were subjected to permanent MCA occlusion, and given either intravenous injection of placebo or 1 mg/kg FK506 immediately after occlusion. DWI and T(2)-weighted MRI were performed 3 and 24 h after MCA occlusion, and postmortem histological analysis was also performed. FK506 drastically reduced the ischemic damage in 3-h apparent diffusion coefficient (ADC) map. This is the first report to demonstrate the neuroprotective effects of FK506 on focal cerebral ischemia in superacute stage. In addition, postmortem ischemic damage tended to be smaller than ischemic area indicated by 3-h ADC map in the FK506 group, whereas there was an excellent equality between them in the placebo group, suggesting the possible effect of FK506 on the later ischemic period. Our findings provide direct evidence for the neuroprotective effect of FK506 on ischemic cell damage in both early stage and possibly later stage. 相似文献
77.
The proton activity of the Nafion membrane was estimated from the potential difference between a normal hydrogen electrode (NHE) in 0.5 mol dm?3 H2SO4 and a dynamic hydrogen electrode (DHE) constructed on a Nafion 117 membrane. The potential difference between the DHE and the NHE was directly measured at the same temperature in a box chamber filled with N2 gas. As a result, the potential difference of E NHE???E DHE changes from 0.006 to ?0.024 V with the increasing temperature from 289 to 313 K; however, the potential difference remains at around ?0.024 V when the temperature is between 313 and 343 K. Based on these data in combination with the Nernst equation, the proton activity of the Nafion 117 membrane at 289 K is estimated to be 0.8, which increases up to ca. 2.4 with the increasing temperature. 相似文献
78.
Proton conductive inorganic–organic hybrid membranes were synthesized from 3-glycidyloxypropyltrimethoxysiane (GPTMS), phenyltriethoxysilane (PhTES) and hydroxyalkylphosphonic acid. Two kinds of hydroxyalkylphosphonic acids, 1-hydroxyethane-1,1-diphosphonic acid (HEDPA) and hydroxyethanephosphonic acid (HEPA), were incorporated into the membranes as functional molecules for proton conduction. FT-IR and Raman studies revealed the presence of phosphonic acid groups in the hybrid membranes. 13C and 29Si NMR confirmed that a three-dimensional siloxane network was formed in the prepared hybrid membrane by hydrolysis and condensation reactions. DTA-TG analysis showed that these membranes were thermally stable up to 200 °C. The HEDPA-based system was found to have higher proton conductivities than the HEPA-based one. The proton conductivities of the hybrid membranes increased with the phosphonic acid content and temperature up to 130 °C. The conductivities of the HEDPA/GPTMS/PhTES membranes = 1/1.6/0.4 were 1.0 × 10−1 and 4.5 × 10−4 S cm−1 at 100% relative humidity and non-humidified conditions, respectively, at 130 °C. 相似文献
79.
Rui Umeda Yuji Shimizu Yuta Ida Masahiro Ikeshita Shuichi Suzuki Takeshi Naota Yutaka Nishiyama 《Tetrahedron letters》2019,60(2):183-186
π-Extended oxepins 1 and dimer 8 were synthesized by the benzannulation of the corresponding asymmetric diarylacetylene derivatives and 2-(phenylethynyl)benzaldehyde followed by the Cu-catalyzed intramolecular cyclization. The optical properties of the π-extended oxepins 1 and 8 are also investigated. 相似文献
80.
Takuji Magata Yoshimi Hirokawa Yusuke Moriguchi Ryosuke Yokoi Shotaro Nojiri Ryota Miyazaki Sayuri Akasako Rina Takahashi Munetaka Nishida Junya Hakoda Ryota Nakayama Aya Tanaka Naoyoshi Maezaki 《Tetrahedron letters》2019,60(10):718-720
A concise, highly stereoselective synthesis of 2,4- and 2,5-disubstituted thiazole amino acids was developed. These are important building blocks for various biologically active thiazole-containing natural peptides and their regioisomeric analogues. The fundamental reactions are diastereoselective addition of (4- or 5-bromothiazol-2-yl)lithium to N-tert-butanesulfinyl imine with subsequent Pd-catalyzed phenoxycarbonylation. 相似文献