Creep and thermal cycling fixture design for metal matrix composites

A creep-thermal cycling system with a constant stress cam has been designed to be inexpensive and easily fabricated in order to identify the creep and thermal-cycling damage mechanisms in metal-matrix composites (MMCs) with high ductility. The current system can be modified to be a stress thermal cycling (or fatigue) system.     

Aptamer-Assisted Blockade of the Immune Suppressor Sialic Acid-Binding Immunoglobulin-Like Lectin-15 for Cancer Immunotherapy

The percentage of low response and adaptive resistance to current antibody-based immune checkpoint blockade (ICB) therapy requires the development of novel immunotherapy strategies. Here, we developed an aptamer-assisted immune checkpoint blockade (Ap-ICB) against sialic acid-binding immunoglobulin-like lectin-15 (Siglec-15), a novel immune suppressor broadly upregulated on cancer cells and tumor infiltrating myeloid cells, which is mutually exclusive of programmed cell death ligand 1 (PD-L1). Using protein aptamer selection, we identified WXY3 aptamer with high affinity against Siglec-15 protein/Siglec-15 positive cells. We demonstrated that WXY3 aptamer rescued antigen-specific T cell responses in vitro and in vivo. Importantly, the WXY3 Ap-ICB against Siglec-15 amplified anti-tumor immunity in the tumor microenvironment and inhibited tumor growth/metastasis in syngeneic mouse model, which may result from enhanced macrophage and T cell functionality. In addition, by using aptamer-based spherical nucleic acids, we developed a synergetic ICB strategy of multivalent binding and steric hindrance, which further improves the in vivo anti-tumor effect. Taken together, our results support Ap-ICB targeted Siglec-15 as a potential strategy for normalization cancer immunotherapy.     

Synthesis, structure, and biological evaluation of C-2 sulfonamido pyrimidine nucleosides

The C-2 sulfonamido pyrimidine nucleosides were prepared by opening the 2,2′- or 2,3′-bond in anhydronucleosides under nucleophilic attack of sulfonamide anions. Reaction of the sodium salt of p-toluenesulfonamide or 2-(aminosulfonyl)-N,N-dimethylnicotinamide with 2,2′-anhydro-1-(β-d-arabinofuranosyl)cytosine gave the C-2 sulfonamido derivatives in excellent yields. Ring opening of the less reactive 2,2′-anhydrouridine and 2,3′-anhydrothymidine could be accomplished with DBU/CH₃CN activation of p-toluenesulfonamide, giving moderate yields for C-2 sulfonamido derivatives. The action of acetic acid or ZnBr₂/CH₂Cl₂ on 5-methyl-N²-tosyl-1-(2-deoxy-5-O-trityl-β-d-threo-pentofuranosyl)isocytosine led to the cleavage of both the protection group and the nucleoside bond, yielding 5-methyl-N²-tosylisocytosine as the major product. Structures of the prepared C-2 sulfonamido nucleosides were confirmed by the 1D and 2D NMR experiments, and X-ray structural analysis of 4-imino-N²-tosylamino-1-(β-d-arabinofuranosyl)pyrimidine. Both methods confirmed β-configuration and anti-conformation of the 2-sulfonamido nucleosides. The investigated compounds displayed moderate inhibition of tumor cell growth in vitro, as determined by the MTT assay using six different human tumor cell lines.     

竖井工法开挖深基坑对下卧既有隧道上浮变形的控制

基坑开挖卸荷将改变地应力平衡状态,位于基坑正下方的地铁隧道将随基底一定深度范围内土层回弹而发生上浮变形。本文结合深圳地铁11号线正上方某采用竖井工法开挖的基坑工程为例,通过建立三维有限元模型分析下卧地铁隧道随竖井开挖过程的变形规律及竖井工法保护机制。结果表明：基坑开挖对下卧地铁隧道竖向卸荷作用显著,采用竖井工法能有效减缓隧道上浮趋势,减小最终上浮量;隧道纵向变形呈双峰形态,纵向变形曲率半径未超过规定值;隧道横截面随开挖过程而发生两侧拱腰压缩、拱顶与拱底之间拉伸的变形趋势,附加弯矩随开挖卸载率增大而逐渐减小,最大附加弯矩位于拱顶附近;竖井工法能减小基底土层的扰动程度,有效抑制基底土体以及隧道围土塑性区发展深度和面积,从而有效控制下卧地铁隧道的隆起量。     

太白洋参中氨基酸和微量元素的分析

报道了太白洋参中氨基酸和微量元素的含量．结果表明：太白洋参中含有人体需要的１５种氨基酸，其中精氨酸的含量相当高；含有７种人体必需的微量元素，５种人体必需的宏量元素．     

Mitochondria Targeting Fluorescent Probes Based on through Bond-Energy Transfer for Mutually Imaging Signaling Molecules H2S and H2O2

Reactive signaling molecules participate in varieties of biochemical reactions, and methods to detect their mutual existence and crosstalk are in urgent demand. A benzothiadiazole-based handle was designed to fluorescently respond to the co-existence of H₂S and H₂O₂ under pseudo-physiological conditions on a basis of a thiyl-radical-mediated mechanism that accounts for the rapid and efficient domino-like reaction processes. Then the handle motif was attached to a rhodamine moiety by means of an ethynylene linkage, and achieved a significant H₂S–H₂O₂ mutual response in the mitochondria of living cells. Theoretical calculations supported that a through bond energy transfer mechanism contributes to the drastic fluorescence response.     

Stabilization of the N-1-substituted cytosinate iminooxo form in dinuclear palladium complexes

The synthesis, as well as chemical and structural characterization, of 1-(p-toluenesulfonyl)cytosine (1) and its dinuclear complexes of the composition Pd₂(1-TosC^?–N3,N4)₄ (3) and Pd₂(1-TosC^?–N3,N4)₂DMSO₂Cl₂ (4) by means of ESI-MS, IR, ¹H NMR and X-ray single crystal analysis are described. Ligand 1 exists in the preferred aminooxo tautomeric form, while both dinuclear complexes reveal the presence of the iminooxo form of the ligand, where 1-TosC^? stands for the anion of the cytosine derivative of 1, bridging two palladium atoms. Complex 3 has a paddlewheel structure with two square-planar coordination spheres, consisting of four N atoms each, mutually parallel and perpendicular to the interpalladium vector. Complex 4 is characterized by two mutually parallel square-planar coordination spheres consisting of two nitrogens, sulfur from the solvent molecule and a chloride. A feasible chemical route for the formation of 3 and 4 via the kinetically favoured mononuclear complex Pd(1-TosC–N3)₂Cl₂ (2) is proposed, based on the IR, ¹H NMR, mass spectrometry, elemental analysis and X-ray structure analysis data.     

用结恢复电流研究a-Si:H p~+-i-n~+结构的缺陷

本文采用多次陷落模型,用结恢复电流技术研究了a—Si:H太阳电池长时间光照后的光致变化效应。发现光照之后的太阳电池比光照前空穴迁移率和寿命的乘积有明显下降,而且光致亚稳缺陷主要起复合中心的作用。     

Influence of Manufacturing Process of Warp-knitted Vascular Prosthesis on the Wall Homogeneity

This paper reports the evolution of textile structure and mechanical properties of vascular prosthesis in the level of the whole prosthesis and the constituent filaments with respect to the manufacturing process. The tubular wall of the prosthesis is divided circumferentially into three zones; basic line (BL), remeshing line ( RL) and guide line ( GL). Some heterogeneity has been observed on the tubular wall in terms of stitch structure of the prosthesis and linear density of the constituent filaments. The breaking position of the prosthesis under circumferential tensile localizes preferentially in remeshing line that is the weakest zone by warp knitting with double needle bed. Furthermore, the statistical differences of the mechanical properties of the filaments of zone RL, GL and BL have been confirmed too. It is predictable that the deterioration of prosthesis, under physiological loads (periodical pulse blood pressure etc.), could happen firstly in the weaker zone in vivo.