Sensitive chemically amplified electrochemical detection of ruthenium tris-(2,2′-bipyridine) on tin-doped indium oxide electrode

Optimized combination of chemical agents was selected for sensitive electrochemical detection of dissolved ruthenium tris-(2,2′-bipyridine) (Ru-bipy). The detection was based on the chemical amplification mechanism, in which the anodic current of a redox-active analyte was amplified by a sacrificial electron donor in solution. On indium-doped tin oxide (ITO) electrodes, electrochemical reaction of the analyte was reversible, but that of the electron donor was greatly suppressed. Several transition metal complexes, such as ferrocene and tris-(2,2′-bipyridine) complexes of osmium, iron and ruthenium, were evaluated as model analyte. A correlation between the amplified current and the standard potential of the complex was observed, and Ru-bipy generated the largest current. A variety of organic bases, acids and zwitterions were assessed as potential electron donor. Sodium oxalate was found to produce the largest amplification factor. With Ru-bipy as the model analyte and oxalate as the electron donor, the analyte concentration curve was linear up to 50 μM, with a lower detection limit of approximately 50 nM. Preliminary work was presented in which a Ru-bipy derivative was attached to bovine serum albumin and detected electrochemically. Although the combination of Ru-bipy, oxalate and ITO electrode has been used before for electrochemiluminescent detection of Ru-bipy and oxalate, as well as electrochemical detection of oxalate, its utility in amplified voltammetric detection of Ru-bipy as a potential electrochemical label has not been reported previously.     

对高校道德实践教育的探讨

文章从道德教育的实践要求、特征、途径等方面，阐述了道德实践在道德教育过程中的地位和作用，并从高校道德教育实际出发，提出了加强道德实践训练的有关措施与方法。     

会计责任与审计责任的差异分析

通过对会计责任与审计责任的概念剖析和会计责任、审计责任界定的国际比较，揭示了会计责任与审计责任在实务中难以界定的原因，指出只有明晰两种责任，才能促进企业的发展和保护注册会计师以及事务所的合法权益。     

VSAT系统改进冲突分解算法研究

文章提出一种新的改进隔离型随机N叉树形冲突分解算法,对VSAT随机接入多址接入系统中冲突分解进行了研究。给出了冲突信息分组分解所需平均时隙数和系统吞吐量的解析式,进一步给出了信息分组冲突分解的实验值。     

碳钢在NaHCO3+NaCl溶液中的亚稳态孔蚀特征

用动电位极化和恒电位极化法研究了A3碳钢在不同浓度NaCl的0.5mol/LNaHCO₃溶液中亚稳态孔蚀行为。实验发现亚稳态电流波动峰具有快速上升、缓慢下降的特点。亚稳孔出现电位E_m 服从正态分布,随着Cl^-浓度的提高,E_m 值向负方向移动。亚稳孔的峰频变化规律与电位关系不大。恒电位极化时,当电位高于E_m而大大低于孔蚀电位E_b 时,电流波动保持一定时间后会最终消失,并产生直径为微米级的小蚀孔。当电位接近孔蚀电位时,一段时间的电流波动后电流往往迅速上升,最终转变为稳定蚀孔.     

基于扫描数据零件轮廓特征自动识别的方法

主要研究基于扫描数据自动识别零件轮廓特征的方法与技术．首先给出了零件轮廓特征自动识别的工作流程，然后提出了零件轮廓特征自动识别的原理与算法，最后通过具体实例验证了本方法的优点及可行性。     

利用有限元法进行塔机起重臂的动态分析

根据塔机起重臂的实际结构 ,建立双弹簧悬吊超静定简支梁的力学模型 ,进行动态分析 ,并以TC80型塔式起重机为例 ,利用有限元方法求解 ,引入梁单元和边界单元 ,得到塔机起重臂的多阶固有频率和主振型 ,其结果对于塔机的动态响应分析具有实际意义     

对数学教育专业课程设置的构想

基础教育数学课程改革已全面启动，师范院校数学教育专业应在分析现在课程设置的基础上，根据数学教师专业化的要求，构建新的课程体系，提出改革的策略和建议，推动基础教育改革的发展．     

数学教育中的学习方法教育

从教学重点、素材来源、方法例示等方面论述了数学教学中学习方法的培养．     

洱海区域新石器时代文化概述

根据现有考古资料,从考古学的角度,概述了洱海区域新石器时代文化遗址的分布与类型、文化特征及文化渊源,证实了该区域新石器文化是在古人类文化的基础上而产生,并不断地汲取其它文化因素,形成了自成一体的区域地方性新石器文化.