Structure and function of annexin V-decorsin fusion

Decorsin is an antagonist of fibrinogen receptor on platelets and Annexin V is able to recognize stimulated platelets. Two recombinant proteins, Annexin V plus Decorsin (AnnV-D39) and Annexin V plus the C terminal 27 amino acids variant of Decorsin (AnnV-D27), were constructed, expressed, and purified. Platelet Aggregation assay results appear that AnnV-D39 shows good anti-platelet aggregation activity, but AnnV-D27 no such activities in any platelet aggregation assay test. And computational simulations reveal that AnnV-D39 showed good anti-platelet aggregation activity as a new antagonist of fibrinogen receptor, while Annv-D27 needs re-modification. Despite the AnnV_D39 fusion is more than decorsin, the former maintains the binding sites of decorsin interacton with its receptor, which contains Asp10, Arg28-Asp33, and Tyr37-Glu39. And the addition of Annexin V could not influence the interaction between its decorsin part with its receptor GPIIb–IIIa due to the linkage peptide (GGGGSGGGGS). Although the AnnV_D27 fusion is similar to the AnnV_D39 fusion, there are differences between them, where the former is in shortage of the linkage peptide and the N-terminal segment of decorsin whose one residue (Asp10) contribution to its interaction with GPIIb–IIIa. Meanwhile, these complex models suggest that decorsin plays a role in antiplatelet aggregation not only by its RGD motif interaction with its GPIIb–IIIa receptor, but also by other residues, especially Asp10 of its N-terminal segment and Tyr37-Glu39 of its C-terminal segment. On the other hand, the linkage peptide acts as avoidance of influence and blockage between domains of fusion. This is the cause that AnnV-D39 shows good anti-platelet aggregation activity.     

C60分子瞬态全光开关

本文以YAG脉冲激光为控制光,He-Ne连续激光为信号光,在C_(60)甲苯溶液中首次实现瞬态全光开关.由光开关时间推断出C_(60)分子三重态的寿命.     

Study of backscattering from a complex scatterer at 3mm wave band

The backscattering characteristics of complex scatterer has been studied theoretically at 3 mm wavelength for the first time. RCS of the practial complex scatterer was calculated with GTD and the physical optics. The constrict model was also measured. The experimental results are in agreement with the theoretical calculations.     

Two New Eremophilenolides from Cacalia pilgeriana

Two new eremophilenolides, 1β-hydroxy-2β-methylsenecioyloxyeremophil-7 （11）-en- 8β （12）-olide （1）, 1β-hydroxy-2β-methylsenecioyloxy-8α-methoxyeremophil-7 （11）-en-8β （12）- olide （2）, were isolated from the roots of Cacalia pilgeriana. Their structures were elucidated by spectroscopic methods and X-ray diffraction analysis.     

Unexpected Synthesis of 2, 2＇-[1-（4-Methoxyphenoxy）propane-2, 2-diyl]bis（5-methoxy-3-methylbenzofuran）

A new bisbenzofuran analogue Ⅶ was achieved unexpectedly in one step procedure from 1-（4-methoxyphenoxy）acetone Ⅰ by using Amberlyst 15 resin as catalyst in excellent yield. The structure was elucidated by spectroscopy analysis including ^1H-NMR, ^13C-NMR, DEPT, ESI-MS, element analysis.     

Homogeneous catalytic asymmetric dihydroxylation of olefins induced by an efficient and recoverable polymer‐bound ligand QN–AQN–OPEG‐OMe

A novel polymer‐bound alkaloid ligand QN–AQN–OPEG‐OMe, which was characterized by an anthraquinone core at the 9‐O position of quinine, was synthesized conveniently. It delivered up to 99% ees in the homogeneous catalytic asymmetric dihydroxylation of seven olefins, comparable to that reported for the homogeneous catalyst with free ligand (DHQ)₂AQN. This ligand was recovered almost quantitatively by a simple filtration and reused for five cycles without obvious decreased enantioselectivities and catalytic activities. Copyright © 2005 John Wiley & Sons, Ltd.     

Two New Butenolide Derivatives from Erigeron acer

IntroductionThe genusErigeron(Compositae)is widelydistributed in Asia,North America and Europe.Thereare about35species distributed in China,some ofwhich have a long history of application in Chinese folkmedicine[1].Sesquiterpenes have been reported as the…     

Modeling research in low-medium temperature geothermal field,Tianjin

The geothermal reservoir in Tianjin can be divided into two parts: the upper one is the porous medium reservoir in the Tertiary system; the lower one includes the basement reservoir in Lower Paleozoic and Middle-Upper Proterozoic. Hot springs are exposed in the northern mountain and confined geothermal water is imbedded in the southern plain. The geothermal reservoir is incised by several fractures. In recent years, TDS of the geothermal water have gone up along with the production rate increasing, along the eastern fracture zone (Cangdong Fracture and West Bai-tangkou Fracture). This means that the northern fracture system is the main seepage channel of the deep circulation geothermal water, and the reservoir has good connection in a certain area and definite direction. The isotopic research about hydrogen and carbon chronology indicates that the main recharge period of geothermal water is the Holocene Epoch, the pluvial and chilly period of 20 kaBP. The karst conduits in weathered carbonate rocks of the Proterozoic and Lower Paleozoic and the northeast regional fracture system are the main feeding channels of Tianjin geothermal water. Since the Holocene epoch, the geothermal water stayed at a sealed warm period. The tracer test in WR45 doublet system shows that the tracer test is a very effective measure for understanding the reservoir’s transport nature and predicting the cooling time and transport velocity during the reinjection. 3-D numerical simulation shows that if the reinjection well keeps a suitable distance from the production well, reinjection will be a highly effective measure to extract more thermal energy from the rock matrix. The cooling of the production well will not be a problem.

     

A DNMR study on the ring inversion of oxazolidines

Ring inversion of eight substituted 2-phenyl-4, 4-dimethyloxazolidines in o-dichlorobenzene-d₄ was studied by ¹H DNMR. Very high inversion barriers (ΔG_o^≠ = 86.7–93.4 kJ·mol^?1) were found for these oxazolidine rings. The magnitude of the inversion barrier is dependent on the polarity of the substituent on the phenyl group. A linear correlation is present between the ΔG_o^≠ of the inversion and the substituent constant, σ+, of the substituent.     

Zeeman原子吸收光谱分析法中谱线轮廓的研究Ⅴ.Ag328.1nm的分析性能

从理论和实验两方面研究和讨论了在ＺＡＡＳ法中，不同调制方式及磁感应强度对Ａｇ３２８．１ｎｍ的分析性能的影响，并取得一致的结果