中国歌剧作品在高校声乐教学中的选择和运用

中国歌剧的历史可以追溯到20世纪20年代,发展至今进入了崭新的阶段.在人们关注下,中国歌剧作品得到普及传唱,均已成为经典.大量中国歌剧声乐作品已进入高等音乐院校声乐专业教学之中,丰富了教学内容,拓宽了学生视野,提高了教学水平.文章分析了20世纪20年代以来中国歌剧作品在声乐教学中选择、运用情况,对未来中国歌剧作品进入教学的问题提出了一定的设想和展望.     

指纹考勤信息管理系统设计与实现

就考勤信息管理信息化的基本情况和安全问题作出了阐述,从需求分析方面做了业务需求分析、数据分析、功能需求分析以及非功能需求分析并采用了数据流程图、业务场景图、用例图来进行分析.系统设计部分做了考勤管理模块设计(考勤时间设置、请假信息管理、出差信息管理、统计查询)、公告信息管理模块设计、系统管理模块设计(用户信息管理、权限管理);并给出了接口设计、数据库概念设计、数据库表设计.     

刑事司法改革：规范刑法司法解释权若干问题再析

提升国家治理体系与治理能力的现代化水平反映在刑事司法领域不仅仅要求司法独立,更要求正确适用刑事法律、法规,规范行使刑法司法解释权。有权机关在行使刑法司法解释权时应严格把握刑法司法解释的司法权能,防止其越位立法,明确刑法司法解释遵循从旧兼从轻的时间效力,彰显刑法谦抑的精神品格,防范突破罪刑法定宪纲性的原则要求。只有刑法得以被正确的适用,才能真正将刑法抽象的正义与公平转化为现实的正义与公平。     

二苯甲烷桥连氮杂环卡宾前体的合成及表征

以4,4′-二氨基二苯甲烷为起始原料,通过碘代、C-N偶联,季铵化,阴离子交换,合成了四种新型桥连的氮杂环卡宾前体(3a,3b和3c和5).产物全部通过核磁表征.     

离散空间直接建模的计算流体力学方法

本文提出了一种发展适合多尺度、多物理流动的CFD格式的全新途径,即直接在离散空间利用物理模型的跨尺度演化解来建立数值格式的新方法.与直接离散偏微分方程的传统做法相比,基于离散空间直接建模构造出的数值方法考虑了网格尺度和物理模型之间的匹配,能做到不同尺度上物理模型的连续过渡,从而实现对多尺度流动的高效模拟.Boltzmann方程的模型演化解为直接建模方法提供了重要的支撑.本文提出了直接建模构造计算流体力学方法的理论基础,并给出了在建立统一气体动理学格式中的成功运用,验证了新方法的可行性和优越性.     

Prototype test study on mechanical characteristics of segmental lining structure of underwater railway shield tunnel

Based on the first unde rwater railway shield tunnel, the Shiziyang shield tunnel of Guangzhou Zhu- jiang River, the prototype test was carried out against its segmental lining structure by using ＂multi-function shield tunnel structure test system＂. And the mechanical characteristics of segmental lining structure using straight assembling and staggered assembling were studied deeply. The results showed that, the mechanical characteristics of segmental lining structure varied with the water pressures; especially after cracking, the high water pressure played a significant role in slowing down the growing inner force and deformation. It also testi- fied that the failure characteristics varied with straight assembling structure and staggered assembling structure. Shear thilurc often occurred near longitudinal seam when using straight assembling.     

Mucoadhesive and elastic films based on blends of chitosan and hydroxyethylcellulose

Mucoadhesive polymeric films have been prepared based on blends of chitosan and hydroxyethylcellulose. The blends have been characterized by IR spectroscopy, DSC, WAXD, TGA, SEM, and mechanical testing. It is demonstrated that the mechanical properties of chitosan are improved significantly upon blending with hydroxyethylcellulose. An increase in hydroxyethylcellulose content in the blends makes the materials more elastic. The thermal treatment of the blends at 100 degrees C leads to partial cross-linking of the polymers and formation of water-insoluble but swellable materials. The adhesion of the films towards porcine buccal mucosa decreases with increasing hydroxyethylcellulose content in the blends.     

A miniaturized glucocorticoid receptor translocation assay using enzymatic fragment complementation evaluated with qHTS

Nuclear translocation is an important step in glucocorticoid receptor (GR) signaling and assays that measure this process allow the identification of nuclear receptor ligands independent of subsequent functional effects. To facilitate the identification of GR-translocation agonists, an enzyme fragment complementation (EFC) cell-based assay was scaled to a 1536-well plate format to evaluate 9,920 compounds using a quantitative high throughput screening (qHTS) strategy where compounds are assayed at multiple concentrations. In contrast to conventional assays of nuclear translocation the qHTS assay described here was enabled on a standard luminescence microplate reader precluding the requirement for imaging methods. The assay uses beta-galactosidase alpha complementation to indirectly detect GR-translocation in CHO-K1 cells. 1536-well assay miniaturization included the elimination of a media aspiration step, and the optimized assay displayed a Z' of 0.55. qHTS yielded EC(50) values for all 9,920 compounds and allowed us to retrospectively examine the dataset as a single concentration-based screen to estimate the number of false positives and negatives at typical activity thresholds. For example, at a 9 microM screening concentration, the assay showed an accuracy that is comparable to typical cell-based assays as judged by the occurrence of false positives that we determined to be 1.3% or 0.3%, for a 3sigma or 6sigma threshold, respectively. This corresponds to a confirmation rate of approximately 30% or approximately 50%, respectively. The assay was consistent with glucocorticoid pharmacology as scaffolds with close similarity to dexamethasone were identified as active, while, for example, steroids that act as ligands to other nuclear receptors such as the estrogen receptor were found to be inactive.     

Bifunctional sensor of nitric oxide and oxygen based on hematite nanotubes embedded in chitosan matrix

A novel hybrid bifunctional sensing platform for simultaneous determination of NO and O₂ has been developed, whereby hematite nanotubes are immobilized into the chitosan matrix onto a gold electrode (labeled as HeNTs-Chi/Au). The HeNTs distributed in porous-structured chitosan matrix not only offer abundant active sites for bifunctional sensing of NO and O₂, but also facilitate oxidation of NO and reduction of O₂ dramatically. Straight calibration curves are achieved in analyte concentration ranges of 5.0 × 10⁻⁸ to 1.25 × 10⁻⁶ mol L⁻¹ for NO and 2.5 × 10⁻⁷ to 6.0 × 10⁻⁶ mol L⁻¹ for O₂. Also, the detection limits are low of 8.0 × 10⁻⁹ mol L⁻¹ for NO and 5.0 × 10⁻⁸ mol L⁻¹ for O₂. Such an efficient bifunctional sensor for NO and O₂ offers great potential in quantitation of NO levels in biological and medical systems, since NO level is highly regulated by various reactive oxygen species.