凝胶微球复合调剖机理实验研究

通过玻璃刻蚀模型进行微观驱替实验,直观地分析了凝胶微球复合调剖过程及作用区域。基于核磁共振技术,设计了凝胶微球岩心驱替实验。对不同阶段驱替后岩心不同直径孔隙内的流体分布进行了研究,并对比凝胶微球复合调剖的优势。实验结果表明,凝胶微球复合调剖过程中凝胶优先进入高渗透层,驱替大孔隙中剩余油,并对大孔隙有封堵作用;则聚合物微球主要进入低渗层,增加了聚合物微球的利用率;并启动低渗透率层的原油,封堵了低渗层的主流通道,实现调剖驱油的双重目的。     

Development and validation of an UPLC-DAD-MS method for the determination of leonurine in Chinese motherwort (Leonurus japonicus)

In the present study, an ultra-performance liquid chromatography method with diode array detection (UPLC-DAD) was developed for the analysis and determination of leonurine from motherwort (Leonurus japonicus), a traditional Chinese medicinal plant. This method was validated in terms of specificity, linearity (R(2) = 0.9995, linear range: 0.005 ~ 0.5 mg/mL), precision (< 5.0% RSD), and recovery (103.2%). The extracted amount of leonurine is 0.15 mg/g. Moreover, the target analyte was identified or tentatively characterized using UPLC coupled with electrospary tandem mass spectrometry (UPLC-ESI-MS).     

第15届固态传感器、传动器与微系统国际会议(Ⅲ)

本届会议的报告续报如下:(1)DV Dao、pH pham、S Saukawa、S suglyama,以棘轮原理与静电传动器为基础的、正切与垂直驱动的微型传输系统;(2)J Barth、EKrevet、M Kohl,具有大输出功率的、双稳自调制放大器的微型传动器;(3)H Ooiso、Y Nishimori、N Fujiwara、T Tuchia、S Machizuoki、G Hashiguehi,一种电梳驱动的传动器的DOF等效电路模型;(4)Y Petremand、W Noell、J F Manceau、N F Rooij、N Golay、A Masse,应用于大冲程排量的、级联式可衡量的快速压紧传动器;(5)S S Je、N Wang、HC Brown、D P Arnold、J Chae,一种与蜡粘结的Nd Fe B微型磁石完全整合在一起的、电磁传动的扬声器及其应用;(6)F Ceyssens、M de Volder、D Reynaerts、R Puers,采用Su-8材料微加工制作的大冲程、强力气动传动器.     

第59届Pittcon会议(Ⅱ)

<正>有关纳米技术的应用报告有:(1)D Rusak,应用发光二极管激发的、纳米粒子增强后的荧光现象;(2)F J Yang,供高通量分析药物与生物试样用的毛细管与纳级高效液相色谱技术的进展;(3)P Baglioni,纳米技术在保护文化遗产方面的     

激发态Cs2和H2的电子-振转能级的碰撞转移

利用相干反斯托克斯拉曼光谱(CARS)探测技术, 研究了激发态Cs2与H2间的电子-振转能级的碰撞转移。用波长为532 nm和中心波长为716 nm的两束激光同时聚焦到样品池中, 扫描CARS谱确认了H2分子的S支(△v =1, △J=2)仅在v=1, J=4,5及v=2, J=3,4能级上有布居, 用n1、n2、n3、n4分别表示(2,4)、(2,3)、(1,4)及(1,5)上的粒子数密度。从CARS线的峰值得到n2/n1、n3/n1、n4/n1分别为6.34±1.27、3.66±0.73和1.45±0.29。转移能配置到振动、转动和平动的比例分别为0.44、0.06和0.50, 能量主要分配在振动和平动上。在T=523 K和PH2=2.5×103 Pa条件下, 通过求解速率方程组和对时间分辨CARS线轮廓的分析, 得到碰撞转移速率系数k1=(6.0±1.2)×10-14 cm-3s-1和k2=(4.0±0.8)×10-13cm-3 s-1。     

激发态H2(X1∑+g,v=1,J=1)之间的碰撞能量合并

受激拉曼激发H2(0,1)基态的Q支得到H2(1,1)能级.在室温条件下,利用反斯托克斯拉曼散射(CARS)探测技术测量了碰撞能量合并(EP)过程H2(1,1)+H2(1,1)→H2(2,J)+H2(0,J″)及H2(1,1)与H2(1,J)转动能级间的碰撞转移速率系数.扫描CARS谱表明在H2(1,1)能级的碰撞能量...     

Studies of Fluorescence Immunosensor Using Eggshell Membrane as Immobilization Matrix

A novel immunosensor using eggshell membrane for determining the human immunoglobulin M (HIgM) in serum was developed. The immunosensor was fabricated by immobilizing goat anti-human IgM antibody on the eggshell membrane with glutaraldehyde. Based on the immunoreactions of goat anti-human IgM (primary antibody), HIgM (target antigen) and the goat anti-human IgM (secondary antibody), the sandwich complex were formed on the eggshell membrane and fluorescein isothiocyanate (FITC) labeling secondary antibody could be employed to detect the target antigen. Under the optimized conditions, the linear range for determining HIgM is 5-60 ng mL(-1) and the detection limit is 4.3 ng mL(-1), which are comparable with the results obtained by general immunonephelometric method. Meanwhile, this proposed sensor also exhibited remarkable storage stability, permeability and highly biocompatibility. The effects of temperature and pH value on eggshell membrane were investigated. Therefore, the proposed immunosensor, by using eggshell membrane as immobilization platform of antibody, offers an excellent fluorescence response to HIgM. The immunosesor provided a new alternative to determine antigens and other bioactive molecules.     

Effect of low level laser irradiation on the proliferation of myoblasts—the skeletal muscle precursor cells: an experimental in vitro study

The aim of this paper is to study the effect of low-level laser irradiation (LLLI) on proliferation of myoblasts in culture. Myoblasts derived from rat skeletal muscle were irradiated by He-Ne laser with different doses. Compared with nonirradiated control group, the number of myoblasts increased when the cells in normal culture conditions were exposed to the laser of specific energy density. The amount of cells with proliferating cell nuclear antigen (PCNA) positive expression and the 5-bromo-2′-deoxyuridine (BrdU) incorporation rate after laser irradiation were also higher than that of the control group, suggesting that LLLL at certain doses can effectively enhance myoblasts growth activity in vitro. This study firstly demonstrated that stimulating myoblasts to enter into proliferative stage from initial resting state was an important mechanism of regeneration and repair of injured skeletal muscle promoted by LLLI in clinical treatment.     

Multi-carrier synthetic aperture communication in shallow water: experimental results

Orthogonal frequency division multiplexing (OFDM) communications in the presence of motion is investigated using data collected from the Kauai Acomms MURI 2008 (KAM08) experiment, conducted off the western side of Kauai, Hawaii, in June-July 2008. The experiment involved a vertical array moored in 106 m deep shallow water and a source towed at a speed of 3 knots at ranges between 600 m and 6 km. In order to attain reliable communications with only a single receive element, a synthetic aperture approach is applied. After combining multiple transmissions, an error-free reception is achieved with a low-density parity-check code, confirming the feasibility of coherent synthetic aperture communications using OFDM.     

Effects of speaking style on speech intelligibility for Mandarin-speaking cochlear implant users

Cochlear implant (CI) users' speech understanding may be influenced by different speaking styles. In this study, speech recognition was measured in Mandarin-speaking CI and normal-hearing (NH) subjects for sentences produced according to four styles: slow, normal, fast, and whispered. CI subjects were tested using their clinical processors; NH subjects were tested while listening to a four-channel CI simulation. Performance gradually worsened with increasing speaking rate and was much poorer with whispered speech. CI performance was generally similar to NH performance with the four-channel simulation. Results suggest that some speaking styles, especially whispering, may negatively affect Mandarin-speaking CI users' speech understanding.