Single-cell discrimination based on optical tweezers Raman spectroscopy

The ability to discriminate between single cells in a label-free and noninvasive fashion is important for the classification of cells, and for the identification of similar cells from different origins. In this paper, we present the Raman spectroscopy-based identifi- cation of different types of single cells in aqueous media, and discrimination between the same types of cells from different donors using a novel Laser Tweezers Raman Spectroscopy (LTRS) technique, which combines laser trapping and micro-Raman spectroscopy. First, we measured the spectra of individual living human erythrocytes, i.e. red blood cells, and leucocytes (U937 cancer cells). High-quality Raman spectra with low fluorescence were obtained using a home-LTRS apparatus and 20 cells were measured for each cell type. The smoothing, baseline subtraction, and normalization of the data were followed by a principal components analysis (PCA). The PCA loading plots showed that the two different types of cells could be completely separated based only on the first component (PC1) (i.e. the peaks at 1300 cm1 ); the discrimination accuracy could therefore reach 100%. More than 50 spectra were taken for each erythrocyte obtained from the four healthy volunteers. The average discrimination accuracy was 84.5% for two random individuals taken from the four volunteers, according to the first and second PCs. This work demonstrates that LTRS is a powerful tool for the accurate identification and discrimination of single cells, and it has the potential to be applied for the highly sensitive identification of cells in clinical diagnosis and medical jurisprudence.     

蔺草的生物学特性

原套由２层细胞组成．包围着内部细胞群—原体．叶原基发生的最初标志是苗端侧面下表皮层细胞的平周分裂。苗中有１个轴及６个节，每节上有１张具鞘的叶．苗中有两个芽，“主芽”由第２叶的叶腋产生，另１个是侧芽由第３叶叶腋产生．合轴分枝．第１—２节是匍匐状，第３—６节直立．茎杆仲长是因居间分生组织活动及细胞增宽与延长的结果．茎杆的不同部位结构有差异．维管束数目自基部向顶部逐渐减少，而髓部空腔宽度则是下部狭，上部宽．割梢及设网支撑可获丰产．     

C60/十八烷酸Langmuir-Blodgett膜滑动摩擦机制的分子动力学模拟

采用分子动力学方法模拟研究了C60/十八烷酸Langmuir-Blodgett（LB）单层膜混合系统的滑动摩擦行为．模拟结果显示：与纯的十八烷酸单层LB膜系统相比,含有C60分子的混合系统具有更低的剪切压．分析结果表明,在滑动过程中,C60分子被局域在由长链分子形成的空穴里,并且在整个滑动过程中发生滚动振荡行为;系统间的摩擦力主要来自于C60分子与十八烷酸单层膜之间的作用力,而十八烷酸单层膜与十八烷酸单层膜之间的作用力很微弱.     

一种用于阳图CTP版材的改性酚醛树脂的合成

以双酚A-甲醛树脂和N,N_二甲基甲酰胺在三氯氧磷的催化作用下发生酯化反应,将酯化产物与乙烯基乙基醚在溶剂存在条件下发生醚化反应,得到双酚A-甲醛树脂的酯醚化合物,经红外光谱确认其已被酯醚化.对产物的红外吸收谱进行研究,该树脂的羟基因被酯醚化其吸收明显减少,在有酸条件下促溶无酸时阻溶,将其应用于热敏阳图CTP版材,证明版材敏度得到提高.     

汽车三轴向转动惯量测量方法研究

根据振动原理提出了一种测量汽车三轴向转动惯量的测量方法，该方法原理简单，可用一种结构紧凑的装置来实现，通过合理地选择系统参数，能获得较高的测量精度。     

氮三乙酸根合铁（Ⅲ）络合物（Fe~ⅢNTA）的光稳定性──Fe~ⅢNTA的光致分子内氧化还原反应的机理

研究了氮三乙酸根合铁（Ⅲ）络合物（ＦｅⅢＮＴＡ）（在２０．０℃、Ｉ为０．５ｍｏｌ·ｋｇ－１（ＮａＣｌＯ４）及ｐＨ值１～２的条件下）对光的稳定性．实验结果表明；随着体系酸度的增加或激发光频率的增大，ＦｅⅢＮＴＡ的光稳定性下降；游离的Ｆｅ（Ⅲ）存在会使ＦｅⅢＮＴＡ的光稳定性增强；混配络合物的生成亦会使较低频率单色光照射下的ＦｅⅢＮＴＡ稳定性下降．还讨论了本体系中ＦｅⅢＮＴＡ的光致分子内氧化还原反应的机理．     

机械能流密度矢量

本文通过对连续媒质内机械能流转移的分析,引入机械能流密度矢量S,并讨论了机械能流在动力学系统中的一些应用。     

Distribution of correlation dimensions of synchronous 12-lead ECG signals

Correlation dimensionsD ₂ of the synchronous 12-lead ECG signals have been investigated for the first time by distributed multi-sensor (multi-electrode) technique. The results show that correlation dimension of heart has the distributed characteristics.D ₂ calculated from different lead ECG signals is not a constant regardless of a healthy person or a coronary heart disease (CHD) patient with sinus rhythm. But with the same lead signal,D ₂ of CHD patients is evidently smaller than that of a healthy person except I, II, III leads. A healthy person and CHD patient can be identified byD ₂ statistically andD ₂ shows the potential application in the diagnosis of the CHD patients.     

MKdV方程孤子扰动的多重时标法

文献[1,2]采用不同方法对带有小扰动项的MKdV方程进行微扰处理,得到了表征MKdV方程单孤子诸参数的绝热演化方程,其结果都只近似到小扰动的一阶.文献[1]     

DK─ⅡA型扭振监测系统

介绍了清华大学热能工程系动力工程及控制实验室研制的ＤＫ－ⅡＡ型扭振监测系统，主要用于大型汽轮发电机组轴系扭振的监测。该系统采用“脉冲时序法”的原理，在齿形盘附近安装非接触式传感器。在传感器信号进入计算机之前，采用了一种独特的数字化处理方法，没有采用Ａ／Ｄ转换器，从而提高了测量精度。系统的测角分辨率为０．００１８°，精度可达０．０１°，而且成本较低。该系统已安装在宁夏大武口发电厂并用于扭振的实时监测