安徽骆冲窑青白瓷化学组成的EDXRF分析研究

骆冲窑始烧于五代时期,是繁昌窑早期阶段的代表性窑口.运用能量色散X射线荧光光谱法(EDXRF)对骆冲窑青白瓷胎、釉样品的化学组成进行分析,并比较了其与唐五代时期的定窑白瓷、五代时期的景德镇窑白瓷和青山窑白瓷的异同;同时对其白度值、显微结构、吸水率和烧成温度等方面进行了考察分析.结果显示,骆冲窑青白瓷与定窑白瓷有很高的相似度,白度值可以媲美白瓷;样品胎体结构致密,已达到较高的烧造温度.     

基于相对测距的水下目标定位算法

在没有星基、地基及水下时空基准信息的辅助下,为解决对水下目标精密定位问题,提出了一种基于水面舰船间相对测距和目标到达角的水下机动目标定位算法(UTPA)。通过联合水面舰船间电磁波相对距离观测和水下声学测角信息建立定位方程,考虑到目标定位方程求解中的异类信息融合权比失配问题,利用Helmert方差分量估计完成目标相对位置的迭代求解。Monte Carlo仿真表明:基于Helmert权比自适应调整的UTPA的定位偏差仅为典型的单平台声学定位技术的10%。     

优化甲硫氨酸补料策略提高重组毕赤酵母G12-CBS合成S-腺苷甲硫氨酸

重组毕赤酵母G12-CBS经代谢工程改造,可高效表达重组S-腺苷甲硫氨酸(SAM)合成酶,并弱化了SAM转化途径的关键酶β-胱硫醚合成酶,是一株优良的SAM高产菌。为了利用G12-CBS高效合成SAM,需对前体(甲硫氨酸)的补料策略进行优化。首先在摇瓶中考察了不同甲硫氨酸(L-Methionine,L-Met)添加量对于G12-CBS的影响,发现每24hL-Met补加量超过3mg/mL时,会影响重组菌生长和SAM合成。在15L发酵罐中优化L-Met的补料速率,当外源补料速率为0.4g/(L·h)时SAM产量最高,分别比补料速率为0.2g/(L·h)和0.6g/(L·h)时提高22.2%和31.8%,达到13.01g/L,比出发菌最高产量提高54%。代谢物及酶活测定的结果表明,较低的L-Met补料速率(0.2g/(L·h))导致前体供应不足而影响SAM合成,过高的补料速率(0.6g/(L·h))可能会抑制三羧酸循环,并且影响氮源的摄取和利用,从而导致菌体生长和产物合成受到抑制。     

海南岛西北部几处新发现的海滩岩及其意义

在海南岛新发现了三处海滩岩露头。本文从岩石学、沉积学和地球化学等方面探讨了它们的成岩环境,结合~(11)C测年分析,估算出琼西北沿海地区4000多年来的平均构造沉降量大于0.5mm/a。     

CVD金刚石膜中的缺陷

研究了利用直流电弧等离子体喷射(DC Arc Plasma Jet)制备的大面积金刚石膜中的缺陷问题.研究表明:金刚石膜中的缺陷包括表面缺陷、晶内缺陷和晶界缺陷,以及由于内应力产生的金刚石膜宏观和微观裂纹.金刚石膜中的缺陷与制备工艺参数有着密切关系.     

基于程序依赖图的Aspect挖掘技术

为了有效地识别遗产代码中因横切关注点而导致的零散代码或交织代码,提出了一种基于程序依赖图的Aspect挖掘方法.根据横切关注点的本质特性,考察了横切关注点与核心关注点之间的依赖关系及其在依赖图上的表现形式,对于单个方法体,结合程序的语义信息,在程序依赖图中引入一个虚拟结点表征方法体的核心功能,所有影响核心功能的语句都与之建立依赖关系,然后对依赖图进行拓扑分析,先从虚拟结点开始分离出核心代码,再通过求解连通分量的方式挖掘方法体内横切代码.实例分析表明,该方法不仅能识别出方法体内的零散特性代码,而且还能有效地挖掘出交织特性的代码.     

动态优化速率和帧长的超宽带链路自适应算法

超宽带系统因发射功率受限,其传输速率对通信距离和信道衰落十分敏感。针对这一特性,以提高媒体访问控制(M AC)层吞吐量为目的,提出了一种基于接收端估计的链路自适应算法。该算法由接收端通过在线动态优化得出实际信道中给定信噪比估值下最佳的传输速率和M AC净荷长度,并通过应答帧附带的方式通知发送端。仿真结果表明,该算法较已有算法提高了吞吐量近30%。该算法复杂度低,对信噪比估计误差不敏感,具有一定的应用潜力。     

A MALDI-MS sensing chip prepared by non-covalent assembly for quantitation of acid phosphatase

A novel sensing chip was designed for MALDI-MS quantitation of acid phosphatase(ACP).The ACP sensing chip was constructed through non-covalent interaction of streptavidin and biotin for the assembly of biotinylated peptide substrate on biotinylated polyethylene-glycol(PEG)modified indium-tin oxide(ITO)slide.In the presence of ACP,the peptide substrate was dephosphorylated under acidic condition to generate a new mass signal.The quantitative assay of ACP was achieved with the mass signal ratio of product to the sum of product and left peptide substrate.Under optimal detection conditions,the ratio was linearly correlated with the concentration of ACP in the range of 0.05–12 g/L with a detection limit(LOD)of 0.04 g/L.The designed ACP sensing chip has been used to analyze ACP in complex clinical samples,which exhibited high selectivity,good repeatability,and admirably anti-interference ability.This work further demonstrates the concept of MS sensing and the application of MALDI-MS in quantitative analysis,and provides a convenient method for the quantitation of proteases in clinical diagnosis.     

Insights into the Allosteric Effect of SENP1 Q597A Mutation on the Hydrolytic Reaction of SUMO1 via an Integrated Computational Study

Small ubiquitin-related modifier (SUMO)-specific protease 1 (SENP1) is a cysteine protease that catalyzes the cleavage of the C-terminus of SUMO1 for the processing of SUMO precursors and deSUMOylation of target proteins. SENP1 is considered to be a promising target for the treatment of hepatocellular carcinoma (HCC) and prostate cancer. SENP1 Gln597 is located at the unstructured loop connecting the helices α4 to α5. The Q597A mutation of SENP1 allosterically disrupts the hydrolytic reaction of SUMO1 through an unknown mechanism. Here, extensive multiple replicates of microsecond molecular dynamics (MD) simulations, coupled with principal component analysis, dynamic cross-correlation analysis, community network analysis, and binding free energy calculations, were performed to elucidate the detailed mechanism. Our MD simulations showed that the Q597A mutation induced marked dynamic conformational changes in SENP1, especially in the unstructured loop connecting the helices α4 to α5 which the mutation site occupies. Moreover, the Q597A mutation caused conformational changes to catalytic Cys603 and His533 at the active site, which might impair the catalytic activity of SENP1 in processing SUMO1. Moreover, binding free energy calculations revealed that the Q597A mutation had a minor effect on the binding affinity of SUMO1 to SENP1. Together, these results may broaden our understanding of the allosteric modulation of the SENP1−SUMO1 complex.     

新型肝素化聚氨酯膜的制备与研究

通过两步溶液聚合法合成表面含有羧基的聚氨酯（PU）,并用1-乙基-3-（二甲基丙胺）碳二亚胺（WSC）对聚氨酯表面羧基进行活化后,在4℃下柠檬酸钠缓冲液中与肝素上氨基反应得到表面共价键接枝的肝素化聚氨酯膜。通过甲苯胺蓝染色法、红外光谱法等对肝素化前后结构特征进行表征,用静态水接触角、体外抗凝血性测试其生物相容性。结果表明：在pH=5.0的肝素柠檬酸钠缓冲液中反应96 h,聚氨酯表面肝素接枝率最高,接枝量达到1.92μg/cm^2;同时,共价接枝肝素后接触角从61°降低到44°,并且抗凝血性能得到明显的改善。