构建适应区域经济发展的职教发展模式--以江西县(市)级职业技术学校发展为例

本文针对近年来江西加快工业化进程，实现江西在中部地区崛起，从而使得各县域经济的持续快速发展对中等职业技术教育这一层次及类型的人才的需求非常旺盛，依据江西县域经济发展的条件、特色，有针对性地提出了江西的县(市)级职业技术学校如何改革与发展的对策。     

一种CDMA2000 1xEV-DO终端物理层软件验证方法

提出了一种可以在计算机上验证CDMA2000 1x EV-DO(EVDO)终端物理层软件的仿真系统.该系统可以模拟实际硬件电路板各个软硬件模块的行为,以及模拟和终端物理层软件相关网络侧的行为,基于此对EVDO终端物理层软件进行仿真验证,提高了新项目的开发效率.     

双酸型离子液体催化废PET醇解制备对苯二甲酸二丁酯研究

针对对苯二甲酸直接酯化合成高值增塑剂对苯二甲酸二丁酯(DBTP)存在的原料价高难得,三废排放量大,催化剂用量大、难分离且不可重复利用等问题,以绿色环保、可循环使用的Brnsted-Lewis双酸型离子液体1-(3-磺酸)-丙基-3-甲基咪唑氯铁酸盐[HO_3S-(CH_2)_3-mim]Cl-FeCl_3(FeCl_3摩尔分数x=0.67)为催化剂,研究了正丁醇醇解廉价易得的废聚对苯二甲酸乙二醇酯(PET)制备增塑剂对苯二甲酸二丁酯的反应.结果表明:在n(PET重复单元)∶n(正丁醇)=1∶3,m(PET)∶m(催化剂)=5∶1,反应温度210℃,反应时间8h的较佳反应条件下,PET醇解率为100%,产物DBTP和乙二醇(EG)收率分别为97.5%和98.2%.同传统催化剂相比,离子液体催化剂的催化性能更高,且重复使用7次其催化性能未见明显降低.傅里叶-红外光谱(FT-IR)分析表明:PET醇解过程由链内酯键断裂和链末端酯键断裂协同完成.     

Jacobi矩阵的逆特征值问题

该文研究如下一类Ｊａｃｏｂｉ矩阵特征值反问题。问题ＩＥＰ：给定ｎ个实数λ１〈λ２〈…〈λｎ,α,β∈｛λｉ,ｉ＝１,…ｎ｝,α≠β,又给定２（「ｎ／２」＋１）个实数ｘｊ,ｙｊ,ｊ＝１,２,…,ｍ－「ｎ／２」）＋１。     

改性纳米碳酸钙补强NR/SBR/BR并用胶的表征

通过固相法在硬脂酸改性商品纳米碳酸钙CCR中加入间苯二酚与六亚甲基四胺的络合物RH,制备了改性纳米碳酸钙M-CCR,并分别制备了天然橡胶/丁苯橡胶/顺丁橡胶并用胶与M-CCR和CCR的复合材料.利用橡胶加工分析仪(RPA)和透射电子显微镜(TEM)研究了M-CCR和CCR在并用胶基体中的分散性、界面结合力、加工性能和形态.同时比较了复合材料的力学性能.结果表明,并用胶填充M-CCR的加工性能、补强作用以及填料的分散性和界面结合力均明显优于CCR.     

Finds in Testing Experiments for Model Evaluation

To evaluate the fault location and the failure prediction models, simulation-based and codebased experiments were conducted to collect the required failure data. The PIE model was applied to simulate failures in the simulation-based experiment. Based on syntax and semantic level fault injections, a hybrid fault injection model is presented. To analyze the injected faults, the difficulty to inject (DTI) and difficulty to detect (DTD) are introduced and are measured from the programs used in the code-based experiment. Three interesting results were obtained from the experiments: 1) Failures simulated by the PIE model without consideration of the program and testing features are unreliably predicted; 2) There is no obvious correlation between the DTI and DTD parameters; 3) The DTD for syntax level faults changes in a different pattern to that for semantic level faults when the DTI increases. The results show that the parameters have a strong effect on the failures simulated, and the measurement of DTD is not strict.     

优化甲硫氨酸补料策略提高重组毕赤酵母G12-CBS合成S-腺苷甲硫氨酸

重组毕赤酵母G12-CBS经代谢工程改造,可高效表达重组S-腺苷甲硫氨酸(SAM)合成酶,并弱化了SAM转化途径的关键酶β-胱硫醚合成酶,是一株优良的SAM高产菌。为了利用G12-CBS高效合成SAM,需对前体(甲硫氨酸)的补料策略进行优化。首先在摇瓶中考察了不同甲硫氨酸(L-Methionine,L-Met)添加量对于G12-CBS的影响,发现每24hL-Met补加量超过3mg/mL时,会影响重组菌生长和SAM合成。在15L发酵罐中优化L-Met的补料速率,当外源补料速率为0.4g/(L·h)时SAM产量最高,分别比补料速率为0.2g/(L·h)和0.6g/(L·h)时提高22.2%和31.8%,达到13.01g/L,比出发菌最高产量提高54%。代谢物及酶活测定的结果表明,较低的L-Met补料速率(0.2g/(L·h))导致前体供应不足而影响SAM合成,过高的补料速率(0.6g/(L·h))可能会抑制三羧酸循环,并且影响氮源的摄取和利用,从而导致菌体生长和产物合成受到抑制。     

基于程序依赖图的Aspect挖掘技术

为了有效地识别遗产代码中因横切关注点而导致的零散代码或交织代码,提出了一种基于程序依赖图的Aspect挖掘方法.根据横切关注点的本质特性,考察了横切关注点与核心关注点之间的依赖关系及其在依赖图上的表现形式,对于单个方法体,结合程序的语义信息,在程序依赖图中引入一个虚拟结点表征方法体的核心功能,所有影响核心功能的语句都与之建立依赖关系,然后对依赖图进行拓扑分析,先从虚拟结点开始分离出核心代码,再通过求解连通分量的方式挖掘方法体内横切代码.实例分析表明,该方法不仅能识别出方法体内的零散特性代码,而且还能有效地挖掘出交织特性的代码.     

A MALDI-MS sensing chip prepared by non-covalent assembly for quantitation of acid phosphatase

A novel sensing chip was designed for MALDI-MS quantitation of acid phosphatase(ACP).The ACP sensing chip was constructed through non-covalent interaction of streptavidin and biotin for the assembly of biotinylated peptide substrate on biotinylated polyethylene-glycol(PEG)modified indium-tin oxide(ITO)slide.In the presence of ACP,the peptide substrate was dephosphorylated under acidic condition to generate a new mass signal.The quantitative assay of ACP was achieved with the mass signal ratio of product to the sum of product and left peptide substrate.Under optimal detection conditions,the ratio was linearly correlated with the concentration of ACP in the range of 0.05–12 g/L with a detection limit(LOD)of 0.04 g/L.The designed ACP sensing chip has been used to analyze ACP in complex clinical samples,which exhibited high selectivity,good repeatability,and admirably anti-interference ability.This work further demonstrates the concept of MS sensing and the application of MALDI-MS in quantitative analysis,and provides a convenient method for the quantitation of proteases in clinical diagnosis.     

Insights into the Allosteric Effect of SENP1 Q597A Mutation on the Hydrolytic Reaction of SUMO1 via an Integrated Computational Study

Small ubiquitin-related modifier (SUMO)-specific protease 1 (SENP1) is a cysteine protease that catalyzes the cleavage of the C-terminus of SUMO1 for the processing of SUMO precursors and deSUMOylation of target proteins. SENP1 is considered to be a promising target for the treatment of hepatocellular carcinoma (HCC) and prostate cancer. SENP1 Gln597 is located at the unstructured loop connecting the helices α4 to α5. The Q597A mutation of SENP1 allosterically disrupts the hydrolytic reaction of SUMO1 through an unknown mechanism. Here, extensive multiple replicates of microsecond molecular dynamics (MD) simulations, coupled with principal component analysis, dynamic cross-correlation analysis, community network analysis, and binding free energy calculations, were performed to elucidate the detailed mechanism. Our MD simulations showed that the Q597A mutation induced marked dynamic conformational changes in SENP1, especially in the unstructured loop connecting the helices α4 to α5 which the mutation site occupies. Moreover, the Q597A mutation caused conformational changes to catalytic Cys603 and His533 at the active site, which might impair the catalytic activity of SENP1 in processing SUMO1. Moreover, binding free energy calculations revealed that the Q597A mutation had a minor effect on the binding affinity of SUMO1 to SENP1. Together, these results may broaden our understanding of the allosteric modulation of the SENP1−SUMO1 complex.