高效液相色谱法快速检测海藻糖

旨在建立一种利用高效液相色谱法检测海藻糖的方法.实验采用Brevibacterium helvolum菌转化液化淀粉生产海藻糖.在反应混合物中,麦芽糖对海藻糖的检测有显著影响.通过改变乙腈和水的比例,麦芽糖和海藻糖最终被完全分开.高效液相色谱条件为：碳水化合物分析柱;洗脱剂为V（乙腈）：V（水）＝80：20;体积流量1 mL/min;进样量20 μL;柱温为室温.麦芽糖和海藻糖的保留时间分别为11.3 min和13.8 min.     

矿区电厂粉煤灰物化特性与吸附特性关联研究

试探讨矿区电厂煤质和燃烧条件对粉煤灰物化特征(粒径分布、比表面积、孔径及孔径分布)的影响,以及粉煤灰物化特性对吸附特性和选择性的影响.研究表明:吸附容量仅与有效吸咐表面积有关;粉煤灰的粒径和孔径分布与灰熔温度ST和煤的变质程度有关;粉煤灰对活性红的吸附在试验范围内,并与langmuir模型相符合.     

利用CRISPR-Cas9系统高效创制水稻品系“17318”的 OsGW2、OsGN1a基因定向突变体

CRISPR-Cas9系统在水稻功能基因的功能研究、性状改良与分子设计育种中起着重要的作用。本研究成功构建了水稻高效定向敲除OsGW2和OsGN1a的CRISPR/Cas9载体pZJP049。并将该载体通过农杆菌介导转化水稻"17318"愈伤,获得水稻再生苗。PCR扩增的方法进行转基因植株阳性检测,结果显示阳性率为100%。PCR-RFLP结果表明OsGN1a基因位点的突变效率为77.7%。PCR-SSCP检测结果显示OsGW2基因位点突变率为100%。双基因聚合突变体(gw2gw2gn1agn1a)的种子在长度和宽度均有明显增加,与预期一致。本研究通过CRISPR-Cas9系统快速聚合Gn1a, GW2基因,实现了目标性状的精确改良。     

Damage evolution and failure mechanism of thermal barrier coatings under Vickers indentation by using acoustic emission technique

Acoustic emission(AE) technique was adopted to monitor the damage evolution of air plasma-sprayed(APS) yttria-partially-stabilized zirconia(YSZ) thermal barrier coatings(TBCs) during instrumented indentation testing, and then the failure mechanisms were investigated by cluster analysis and wavelet transform methods.The results of cluster analysis showed that there were three classes associated with distinct failure types for the 8YSZ coatings under Vickers indentation. Based on wavelet transform, these three clusters could be clearly distinguished from their dominant frequency bands, which were concentrated on levels A5(0–156.25 kHz), D5(156.25–312.5 kHz) and D4(312.5–625 kHz), respectively. Thus, the failure mechanism of 8YSZ coatings under Vickers indentation could be clarified by the distribution of different failure types in indentation depth. To sum up, as indentation load increases, the 8YSZ coatings can accommodate the indenter by elastic or little plastic deformation, microcracks propagation and then debonding at the splat boundaries. By comparing the distribution of AE signals induced by different failure types in indentation depth for samples with different thermal exposure time, it can be inferred that thermal exposure treatment can accelerate the degradation of APS 8YSZ TBCs.     

n-星上自同胚的迭代根

设n是个自然数,Xn={z∈C:zn∈[0,1]}是个n-星,F是Xn上的连续自映射.若存在Xn上的连续自映射f及自然数m≥2,使得fm=F,则称f是F的一个m阶迭代根.本文得到了Xn上任一自同胚具有m阶迭代根的条件.     

酶联免疫法测定水产品中己烯雌酚残留量

采用酶联免疫法测定水产品中残留己烯雌酚的含量,并采用液相色谱-质谱联用法验证阳性结果。实验结果表明,该方法对水产品中己烯雌酚的检出限为0.35 μg/kg,回收率达77%～91%,具有良好的灵敏度和准确度。     

聚乙二醇辅助水热合成铁酸铋粉体及其光催化性能

在聚乙二醇(PEG-4000)辅助下,利用水热法以硝酸铋(Bi(NO3)3.5H2O)和硝酸铁(Fe(NO3)3.9H2O)为反应原料,KOH为矿化剂,180℃反应6h合成了不同形貌及尺寸的铁酸铋(BiFeO3)粉体,探索了PEG-4000的添加量(0、0.1、0.2、0.3mmol)对反应产物形貌及尺寸的影响.利用X射线衍射仪、红外吸收光谱仪、扫描电子显微镜、紫外可见光吸收光谱仪等对制备的BiFeO3粉体的相结构、形貌及光吸收特性进行了表征.结果表明:添加0.1mmol和0.3mmol的PEG-4000,可以得到颗粒尺寸小、形貌规整的单相钙钛矿BiFeO3粉体,即适当的添加量对产物的形貌及尺寸影响较大.UV-Vis吸收图谱表明,随着颗粒尺寸的减小,粉体的吸收带边发生了蓝移.进一步以光降解亚甲基蓝(MB)溶液,研究了制备的BiFeO3粉体的光催化性能.结果显示:所得粉体表现出良好的光催化活性,且随着粒径的减小,催化效率提高.     

Temporal precision in the neural code and the timescales of natural vision

The timing of action potentials relative to sensory stimuli can be precise down to milliseconds in the visual system, even though the relevant timescales of natural vision are much slower. The existence of such precision contributes to a fundamental debate over the basis of the neural code and, specifically, what timescales are important for neural computation. Using recordings in the lateral geniculate nucleus, here we demonstrate that the relevant timescale of neuronal spike trains depends on the frequency content of the visual stimulus, and that 'relative', not absolute, precision is maintained both during spatially uniform white-noise visual stimuli and naturalistic movies. Using information-theoretic techniques, we demonstrate a clear role of relative precision, and show that the experimentally observed temporal structure in the neuronal response is necessary to represent accurately the more slowly changing visual world. By establishing a functional role of precision, we link visual neuron function on slow timescales to temporal structure in the response at faster timescales, and uncover a straightforward purpose of fine-timescale features of neuronal spike trains.     

阻尼系数的测定

利用数值计算方法,将实验数据得出的拟合曲线和振动方程的解析解、数值解进行比较,从而确定阻尼振动系统的阻尼系数.此方法简单易行,容易推广到其他类似问题的研究,具有一定的推广价值.     

THE蛋白的结构分析与功能预测

调用motif数据库、profile数据库和interproscan数据库,对THE蛋白进行了序列同源性分析和功能位点分析.结果表明,THE蛋白质是一种核蛋白,理论等电点为6.36,分子量为44 859 Dalton.应用多种相关软件对THE蛋白的二级结构和特殊结构进行了初步预测,结果显示:THE蛋白中存在α螺旋、β-折叠片和无规卷曲结构,有两个可形成跨膜结构的片段,不存在卷曲螺旋,无信号肽,也无线粒体定位信号.对THE蛋白进行序列同源性、结构域及功能位点预测,结果显示:THE蛋白与来自大鼠睾丸的Tes13-S、Fos13-L和几种假设蛋白有较高的相似性;THE蛋白存在次黄嘌呤核苷酸脱氢酶、嘌呤核苷酸还原酶结构域及PKC、酰胺化、豆蔻酸连接等功能位点,无糖基化位点.THE蛋白的结构分析与功能预测为该基因的功能研究提供了重要的依据.