光电池在光强测量中的最佳使用条件

从理论和实验上探讨了硅光电池的光电转换性能。推导了在光强测量实验中光电池光生伏特和光生电流与外接测量线路电阻的关系。指出了硅光电池的最佳使用条件,并设计出测量线路。     

基于OAI-PMH的服务提供者的设计与实现

设计和实现一个基于OAI-PMH的服务提供者.讨论目前数字图书馆中异构数据的检索现状,分析OAI-PMH协议及其互操作框架,基于OAI-PMH协议设计服务提供者系统框架,并提出了OAI-PMH互操作框架中数据快速更新的推/拉模型.完成服务提供者框架中批量获取数据提供者基地址、元数据收获、统一检索3个主要模块的设计与实现.该系统实现了分布异构数据的获取和在此基础上的集中式元数据统一检索,支持数字图书馆中分布式信息资源的可互操作和对用户的透明性,作为数字图书馆中统一检索的一种实现方式.     

基于参数微扰法精确计算铍离子Be2+和硼离子B3+的基态能量

建立含有屏蔽参数σ的铍离子Be~(2+)(z=4)和硼离子B~(3+)(z=5)的哈密顿方程.以有效核电荷数为z*=z-σ的类氢原子基态1s和激发态ns(n=2,3,…)组合的双电子波函数,作为铍离子Be~(2+)和硼离子B~(3+)的基态近似波函数.应用参数微扰法确定铍离子Be~(2+)的屏蔽参数σ=0.56350197和硼离子B~(3+)的屏蔽参数σ=0.525070444,应用参数微扰法计算铍离子Be~(2+)三级近似基态能量和硼离子B~(3+)四级近似基态能量的理论值与实验值的误差为ΔE≈10~(-4)~10~(-5)a.u..     

基于协同克里格插值法的年均温空间插值的参数选择研究

为了确定可能的参数设定对协同克里格插值结果的影响,本文以福建省66个气象站点的2007年平均气温为例,在ArcGIS软件地统计模块的支持下,采用交叉检验方法对插值过程中半变异函数的最优理论模型、最佳分割距离、基台和块金以及空间数据变异方向性等参数和模型的选择进行了影响分析.结果表明,考虑高程影响因素的协同克里格（Cokriging）插值结果可以较真实的模拟气象的空间分布特征,而在插值时选用什么样的模型和参数对插值结果影响较大,为此,应综合数据和研究区的特征,对相关模型和参数进行合理的选择,进而获得最小的拟合误差和最优的插值结果.     

平衡式变量叶片泵的动力学建模和仿真

为降低汽车液压动力转向系统中系统转向泵的能量损失,提出一种含有浮动块的新型平衡式变量叶片泵.采用复平面矢量数学分析法研究平衡式变量叶片泵的动力学特性,建立了平衡式变量叶片泵变量机构的动力学模型,并用ADAMS软件对平衡式变量叶片泵变量机构进行动力学仿真.结果表明,浮动块体位移等变量能够较精确地反映变量机构运动规律,验证了该模型的有效性.     

Rutin Inhibits Ox-LDL-Mediated Macrophage Inflammation and Foam Cell Formation by Inducing Autophagy and Modulating PI3K/ATK Signaling

Atherosclerosis (AS) is one of the leading causes of death among the elderly, and is primarily caused by foam cell generation and macrophage inflammation. Rutin is an anti-inflammatory, anti-oxidant, anti-allergic, and antiviral flavonoid molecule, known to have anti-atherosclerotic and autophagy-inducing properties, but its biological mechanism remains poorly understood. In this study, we uncovered that rutin could suppress the generation of inflammatory factors and reactive oxygen species (ROS) in ox-LDL-induced M2 macrophages and enhance their polarization. Moreover, rutin could decrease foam cell production, as shown by oil red O staining. In addition, rutin could increase the number of autophagosomes and the LC3II/I ratio, while lowering p62 expression. Furthermore, rutin could significantly inhibit the PI3K/ATK signaling pathway. In summary, rutin inhibits ox-LDL-mediated macrophage inflammation and foam cell formation by inducing autophagy and modulating PI3K/ATK signaling, showing potential in treating atherosclerosis.     

The Pharmacokinetics in Mice and Cell Uptake of Thymus Immunosuppressive Pentapeptide Using LC-MS/MS Analysis

Thymus immunosuppressive pentapeptide (TIPP) is a novel anti-inflammatory peptide with high efficacy and low toxicity. This study aims to establish a selective LC-MS/MS method for analyzing the analyte TIPP in biological samples, laying the foundation for further PK and PD studies of TIPP. Protein precipitation was conducted in acetonitrile supplemented with 2% formic acid and 25 mg/mL dithiothreitol as a stabilizer, which was followed by backwashing the organic phase using dichloromethane. The chromatographic separation of TIPP was achieved on a C18 column with a gradient elution method. During positive electrospray ionization, TIPP was analyzed via multiple-reaction monitoring. The linear relationships between the concentration of TIPP and peak area in murine plasma cell lysates, supernatants, and the final cell rinse PBS were established within the ranges of 20–5000 ng/mL, 1–200 ng/mL, 10–200 μg/mL, and 0.1–20 ng/mL, respectively (r² > 0.99). Validated according to U.S. FDA guidelines, the proposed method was proved to be acceptable. Such a method had been successfully applied to investigate the pharmacokinetics of TIPP in mice via subcutaneous injection. The plasma half-life in mice was 5.987 ± 1.824 min, suggesting that TIPP is swiftly eliminated in vivo. The amount of TIPP uptake by RBL-2H3 cells was determined using this method, which was also visually verified by confocal. Furthermore, the effective intracellular concentration of TIPP was deduced by comparing the intracellular concentration of TIPP and degrees of inflammation, enlightening further investigation on the intracellular target and mechanism of TIPP.     

Insights into the Allosteric Effect of SENP1 Q597A Mutation on the Hydrolytic Reaction of SUMO1 via an Integrated Computational Study

Small ubiquitin-related modifier (SUMO)-specific protease 1 (SENP1) is a cysteine protease that catalyzes the cleavage of the C-terminus of SUMO1 for the processing of SUMO precursors and deSUMOylation of target proteins. SENP1 is considered to be a promising target for the treatment of hepatocellular carcinoma (HCC) and prostate cancer. SENP1 Gln597 is located at the unstructured loop connecting the helices α4 to α5. The Q597A mutation of SENP1 allosterically disrupts the hydrolytic reaction of SUMO1 through an unknown mechanism. Here, extensive multiple replicates of microsecond molecular dynamics (MD) simulations, coupled with principal component analysis, dynamic cross-correlation analysis, community network analysis, and binding free energy calculations, were performed to elucidate the detailed mechanism. Our MD simulations showed that the Q597A mutation induced marked dynamic conformational changes in SENP1, especially in the unstructured loop connecting the helices α4 to α5 which the mutation site occupies. Moreover, the Q597A mutation caused conformational changes to catalytic Cys603 and His533 at the active site, which might impair the catalytic activity of SENP1 in processing SUMO1. Moreover, binding free energy calculations revealed that the Q597A mutation had a minor effect on the binding affinity of SUMO1 to SENP1. Together, these results may broaden our understanding of the allosteric modulation of the SENP1−SUMO1 complex.     

基于MCE-CA-Markov模型的佛山市土地利用景观格局演变及模拟预测

以佛山市2005,2010和2015年的Landsat遥感影像为数据源,从景观生态学的角度分析了2005―2015年佛山市各土地利用景观类型的分布格局、空间演变和景观格局变化特征,并结合自然和社会2大影响因素,基于MCE-CA-Markov模型,利用IDRISI软件实现了对佛山市未来土地利用景观格局的模拟和预测﹒在此基础上,结合城市未来的发展变化情况提出了相应的管控建议﹒     

膨润土-聚乙烯醇高吸水性复合树脂的合成及性能研究

采用悬浮聚合法 ,以顺丁烯二酸酐为交联剂 ,添加膨润土 ,制得聚乙烯醇高吸水性复合树脂 .该树脂的吸水倍率和吸盐水倍率分别为 3 60和 3 7倍 .通过实验 ,讨论了交联剂用量、膨润土添加量、水解程度等因素对吸水性能的影响 .