Cloning and functional analysis of chloroplast division gene NtFtsZ2-1 in Nicotiana tabacum

FtsZ protein plays an important role in the division of chloroplasts. With the finding and functional analysis of higher plant FtsZ proteins, people have deepened the understanding in the molecular mechanism of chloroplast division. Multiple ftsZ genes are diversified into two families in higher plants, ftsZ1 and ftsZ2. On the basis of the research on ftsZ1 family, we analyzed the function of NtFtsZ2-1 gene in Nicotiana tabacum. Microscopic analysis of the sense and antisense NtFtsZ2-1 transgenic tobacco plants revealed that the chloroplasts were abnormal in size and also in number when compared with wild-type tobacco chloroplasts. Our investigations confirmed that the NtFtsZ2-1 gene is involved in plant chloroplast division.     

石墨炉平台原子吸收法测定人体体液中的银

该文介绍使用石墨炉平台原子吸收分光光度法,并采用塞曼效应进行背景校正,以准确测定人体血液及尿液中银的含量,研究结果表明,方法回收率分别为93.7%～100%和92.7%～101%,方法检测限分别为0.4 μg/L和0.2 μg/L,批内相对标准偏差分别为8.4%和3.4%.该方法准确度高,容易掌握,符合检测要求,可用于环境和医学银的生物监测.     

阿根廷库约和内乌肯盆地油气特征与勘探潜力

位于阿根廷中西部地区的库约盆地和内乌肯盆地是南美次安第斯盆地群南段的两个较独特的弧后前陆盆地。俯冲角度的变化导致二者岩石圈及冲断带构造的不同。两个盆地经历了相似的4个盆地演化阶段:基底、裂谷期、后裂谷沉降期、前陆期。库约盆地发育一个已证实的含油气系统,以上三叠统富有机质湖相泥岩为主力烃源岩,主要以三叠系河流相砂砾岩为储层,盖层为三叠系层内盖层和白垩系区域盖层,圈闭形成和油气生成高峰在新近纪。内乌肯盆地发育1个已证实的和3个推测的含油气系统,除裂谷期上三叠统源岩外,其余下—中侏罗统、凡兰吟阶—巴列姆阶、提塘阶—凡兰吟阶的源岩均发育在裂谷后沉降单元内,储层范围从二叠系至新生界,最富产层为上侏罗统—下白垩统砂岩和灰岩储层,油气系统关键时刻为晚白垩世—新近纪。海陆相沉积环境的不同造成烃源岩质量的巨大差异,直接体现在其成藏组合中赋存悬殊的油气资源量;各阶段中相对独立的构造–沉积环境造成了油气地质特征与勘探潜力的差异。     

3D Multiphase Piecewise Constant Level Set Method Based on Graph Cut Minimization

Segmentation of three-dimensional (3D) complicated structures is of great importance for many real applications. In this work we combine graph cut minimization method with a variant of the level set idea for 3D segmentation based on the Mumford-Shah model. Compared with the traditional approach for solving the Euler-Lagrange equation we do not need to solve any partial differential equations. Instead, the minimum cut on a special designed graph need to be computed. The method is tested on data with complicated structures. It is rather stable with respect to initial value and the algorithm is nearly parameter free. Experiments show that it can solve large problems much faster than traditional approaches.     

Investigation of the Interaction Between N-benzyl Piperidones and Bovine Serum Albumin by Spectroscopic Approaches

Fluorescence and ultraviolet spectroscopies were applied to investigate the interaction between five N-benzyl piperidones (NBP) and bovine serum albumin (BSA) under imitated physiological conditions. The experimental results show that these NBPs have a static fluorescence quenching effect on the endogenous fluorescence of BSA at the temperatures 302 and 310?K. According to the fluorescence quenching theory, the Stern?CVolmer quenching constant (K _SV), the binding constant (K _a), and the number of binding sites (n) were obtained. Also, the effect of substituents on the binding capacity between NBP and BSA is in the order: C₆H₅?<?4-CH₃OC₆H₄?<?4-CH₃C₆H₄?<?2,4-Cl₂C₆H₃?<?4-ClC₆H₄. The corresponding thermodynamic parameters (??G, ??H, and ??S) were obtained at two different temperatures and indicate that hydrogen bonding and van der Waals forces play main roles in stabilizing the BSA?CNBP complex. The binding distance between the tryptophan residue in BSA and NBP was found to be much <7?nm based on the F?rster theory of non-radiation energy transfer. The effect of NBP on the conformation of BSA was analyzed as well by synchronous fluorescence spectroscopy.     

基于Asp.net的通用数据填报系统的设计与实现

本文结合实际应用,详细阐述了一种基于Asp.net的通用数据填报系统的设计与实现。该系统能够对数据表字段进行任意定义,并动态生成相应数据存储、Excel模板及界面程序,从而更灵活的进行数据填报、查询和导出。解决了传统管理信息系统扩展性差的问题,具有一定的先进性、实用性。     

彩虹自标定方法对混合喷雾比例的原位测量

传统彩虹测量技术标定复杂、精度可重复性差且难以适应密闭空间。一种基于多彩虹谱线自标定方法,可省去原有的标定系统装置,将标定过程内化于对彩虹信号的处理,以水为例的测量偏差仅为0.13%。将该技术应用于混合喷雾液滴组分浓度测量中,探究并优化了参与混合的两种液体组分的方案。实验采用60%乙醇-水混合喷雾,原位测量了混合喷雾场多点组分浓度,测量结果差别可以控制在0.5%以内,表明该方法技术在相关领域的应用潜力。     

Electrochemical Characteristics of Discrete,Uniform, and Monodispersed Hollow Mesoporous Carbon Spheres in Double‐Layered Supercapacitors

Core–shell‐structured mesoporous silica spheres were prepared by using n‐octadecyltrimethoxysilane (C18TMS) as the surfactant. Hollow mesoporous carbon spheres with controllable diameters were fabricated from core–shell‐structured mesoporous silica sphere templates by chemical vapor deposition (CVD). By controlling the thickness of the silica shell, hollow carbon spheres (HCSs) with different diameters can be obtained. The use of ethylene as the carbon precursor in the CVD process produces the materials in a single step without the need to remove the surfactant. The mechanism of formation and the role played by the surfactant, C18TMS, are investigated. The materials have large potential in double‐layer supercapacitors, and their electrochemical properties were determined. HCSs with thicker mesoporous shells possess a larger surface area, which in turn increases their electrochemical capacitance. The samples prepared at a lower temperature also exhibit increased capacitance as a result of the Brunauer–Emmett–Teller (BET) area and larger pore size.     

基于IFCM-GRA的空间多维热误差温度测点优化

热误差是精密、超精密加工中主要的误差源之一,热误差温度测点优化是热误差补偿的关键问题.在机床空间多维布置的大量温度测点之间存在多重相关性,从众多测点中选取特征点的优劣程度,将直接影响到热误差补偿效果.通过对温度测点间多重相关性及温度与热误差关系的综合分析,采用改进的模糊C-均值(IFCM)聚类算法对温度测点进行聚类,以减小类与类之间温度测点的相关性,且避免FCM算法对初始聚类中心敏感易局部收敛的缺点.对温度测点按灰色关联分析(GRA)中的灰色综合关联度进行排序,从变化量和变化率的角度综合反映温度与热误差的关系.采用IFCM-GRA对温度测点进行优化,提高了热误差模型的鲁棒性及准确性,使温度测点数量大幅度减少.在某型号精密卧式加工中心上进行实验,温度测点从17个减少到4个.在不同转速下,利用多元线性回归对优化出的温度测点与热误差建立模型,所建立模型均能很好地预测热误差变化情况,经对预测模型分析,轴向热误差由几十微米减小到5μm以内.